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US5354854A
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Expression system for use in plants to suppress foreign expression and method
Assignee: University of Missouri-Columbia
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Filing: 1991-11-07
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Granted: 1994-10-11
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Expires: 2011-11-07
Abstract
Abstract An expression system and method for using the same in plants to suppress gene expression, the system including a constitutive promoter element from a tRNA gene and an antisense strand DNA fused to the promoter element for being co-transcribed with the promoter element by an RNA polymerase III to suppress expression of a gene.
Inventors
- 👤 June E. Bourque
- 👤 William R. Folk
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 21:23
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1137; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8218; C12Y; C12Y203/00; C12Y203/01; C12Y203/01028; C07; C07K; C07K2319/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
68 words
An expression system for use in plants to suppress foreign gene expression, said system consisting essentially of: a constitutive promoter element from a plant tRNA methionine initiator (tRNA met ) gene, said promoter element lacking a terminator, and a DNA sequence in reverse orientation fused to said promoter element for being co-transcribed with said promoter element by an RNA polymerase III to inhibit expression of the foreign gene.
Claim 2
Dependent Claim
68 words
An expression system for use in plants to suppress foreign gene expression, said system consisting essentially of: a constitutive promoter element from a plant tRNA methionine initiator (tRNA met ) gene, said promoter element lacking a terminator, and a DNA sequence in reverse orientation fused to said promoter element for being co-transcribed with said promoter element by an RNA polymerase III to inhibit expression of the foreign gene.
Claim 3
Dependent Claim
28 words
A system as set forth in claim 1 wherein said tRNA met i gene is derived from soybean said gene consisting essentially of 72 bp of coding sequence.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-10 | 1998-04-11 | 1998-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-10 | 2002-04-11 | 2002-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-10 | 2006-04-11 | 2006-10-11 | $7700.00 | Window Closed / Lapsed |