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US5349126A
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US
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Process and composition for increasing squalene and sterol accumulation in higher plants
Assignee: BP Corp North America Inc
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Filing: 1992-08-14
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Granted: 1994-09-20
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Expires: 2012-08-14
Abstract
Abstract A process of increasing squalene and sterol accumulation in a transgenic plant by increasing the amount of a gene encoding a polypeptide having HMG-CoA reductase activity is disclosed. The amount is preferably increased by transforming plant cells with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide to form a transformed plant cell and regenerating a transgenic plant from that transformed cell. Also disclosed are a process of increasing pest resistance in a transgenic plant, transgenic plants and transgenic seeds capable of germinating into transgenic plants.
Inventors
- 👤 Joseph Chappell
- 👤 Court A. Saunders
- 👤 Fred R. Wolf
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:49
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8286; Y; Y02; Y02A; Y02A40/00; Y02A40/10; Y02A40/146
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
79 words
A process of increasing insect resistance in a transgenic plant said process comprising: (a) transforming a plant cell with a recombinant DNA molecule comprising a vector operatively linked to a DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide in said plant cell to form a transformed plant cell; and (b) regenerating said transformed plant cell into said transgenic plant wherein said plant exhibits increased insect resistance.
Claim 2
Dependent Claim
79 words
A process of increasing insect resistance in a transgenic plant said process comprising: (a) transforming a plant cell with a recombinant DNA molecule comprising a vector operatively linked to a DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide in said plant cell to form a transformed plant cell; and (b) regenerating said transformed plant cell into said transgenic plant wherein said plant exhibits increased insect resistance.
Claim 3
Dependent Claim
38 words
The process according to claim 1 wherein said polypeptide comprises the HMG-CoA reductase catalytic region and at least a portion of the HMG-CoA reductase linker region but is free from the membrane binding region of a HMG-CoA reductase.
Claim 4
Dependent Claim
27 words
The process according to claim 1 wherein the promoter is a promoter whose regulatory function is substantially unaffected by the level of sterol in said transgenic plant.
Claim 5
Dependent Claim
14 words
The process according to claim 1 wherein the promoter is the CaMV 35S promoter.
Claim 6
Dependent Claim
30 words
The process according to claim 1 wherein said plant cell is obtained from plants of the group consisting of tobacco, cotton, soybean, tomato, alfalfa arabidopsis, corn, barley, carrot and guayule.
Claim 7
Dependent Claim
12 words
A transgenic plant produced in accordance with the process of claim 1.
Claim 8
Dependent Claim
48 words
A plant whose genome comprises a DNA structural gene, said structural gene consisting essentially of an HMG-CoA reductase catalytic region and at least a portion of an HMG-CoA reductase linker region, said plant exhibiting increased insect resistance in comparison with a native plant not containing said structural gene.
Claim 9
Dependent Claim
48 words
A plant whose genome comprises a DNA structural gene, said structural gene consisting essentially of an HMG-CoA reductase catalytic region and at least a portion of an HMG-CoA reductase linker region, said plant exhibiting increased insect resistance in comparison with a native plant not containing said structural gene.
Claim 10
Dependent Claim
25 words
A plant according to claim 7 which is selected from the group consisting of: arabidopsis, tobacco, cotton, soybean, tomato, alfalfa, corn, barley, carrot, and guayule.
Claim 11
Dependent Claim
31 words
A method of making a plant having increased insect resistance, said method comprising crossing a plant according to claim 7 with a second plant, and selecting progeny having increased insect resistance.
Claim 12
Dependent Claim
54 words
A seed, which upon germination produces a plant whose genome comprises a DNA structural gene, said structural gene consisting essentially of an HMG-CoA reductase catalytic region and at least a portion of an HMG-CoA reductase linker region, said plant exhibiting increased insect resistance in comparison with a native plant not containing said structural gene.
Claim 13
Dependent Claim
54 words
A seed, which upon germination produces a plant whose genome comprises a DNA structural gene, said structural gene consisting essentially of an HMG-CoA reductase catalytic region and at least a portion of an HMG-CoA reductase linker region, said plant exhibiting increased insect resistance in comparison with a native plant not containing said structural gene.
Claim 14
Dependent Claim
24 words
A seed according to claim 10 which is selected from the group consisting of: tobacco, cotton, soybean, tomato, alfalfa, corn, barley, carrot, and guayule.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1997-09-19 | 1998-03-21 | 1998-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-09-19 | 2002-03-21 | 2002-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-09-19 | 2006-03-21 | 2006-09-20 | $7700.00 | Window Closed / Lapsed |