Patent US5336412A successfully refreshed from Google Patents.
US5336412A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Push column chromatography method
Assignee: Agilent Technologies Inc
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Filing: 1993-06-28
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Granted: 1994-08-09
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Expires: 2013-06-28
Abstract
Abstract A method for chromatography of DNA, RNA, proteins and other molecules includes the use of a column adapted to hold a chromatography material and a sample to be filtered. A pneumatic pressure differential is applied across the column and the sample is urged through the chromatography material. A selected portion of the sample may then be collected.
Inventors
- 👤 William D. Huse
- 👤 Anthony M. Sorge
- 👤 Keith V. Sylvester
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 12:12
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/34; C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12N15/101; G; G01; G01N; G01N1/00; G01N1/28; G01N1/40; G01N1/405; G01N30/00; G01N30/02; G01N30/26; G01N30/28; G01N30/32; G01N30/60; G01N30/6047
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
125 words
A chromatography method for separating DNA or RNA fragments having a molecular size range from unincorporated nucleotides having a smaller molecular size range, said method comprising the steps of: providing a column with a gel chromatography material having an internal porosity selected intermediate between the molecular size range of the DNA or RNA fragments and the molecular size range of the unincorporated nucleotides; introducing into the column a sample of the DNA or RNA fragments along with unincorporated nucleotides; attaching a syringe to one end of the column; using the syringe to induce a pneumatic pressure differential across said column sufficient to cause said sample to pass through said chromatography material; and collecting eluent containing said DNA or RNA fragments substantially free of unincorporated nucleotides.
Claim 2
Dependent Claim
125 words
A chromatography method for separating DNA or RNA fragments having a molecular size range from unincorporated nucleotides having a smaller molecular size range, said method comprising the steps of: providing a column with a gel chromatography material having an internal porosity selected intermediate between the molecular size range of the DNA or RNA fragments and the molecular size range of the unincorporated nucleotides; introducing into the column a sample of the DNA or RNA fragments along with unincorporated nucleotides; attaching a syringe to one end of the column; using the syringe to induce a pneumatic pressure differential across said column sufficient to cause said sample to pass through said chromatography material; and collecting eluent containing said DNA or RNA fragments substantially free of unincorporated nucleotides.
Claim 3
Dependent Claim
18 words
A chromatography method as recited in claim 1 wherein the gel chromatography material is a polysaccharide or polyacrylamide.
Claim 4
Dependent Claim
91 words
A chromatography method for separating polyadenylated RNA from nonpolyadenylated RNA, said method comprising the steps of: providing a column with a chromatography material including oligo(dT)-cellulose; introducing into the column a sample of polyadenylated RNA and nonpolyadenylated RNA; attaching a syringe to one end of the column; using the syringe to induce a pneumatic pressure differential across said column sufficient to cause said sample to pass through said chromatography material; and collecting eluent containing said nonpolyadenylated RNA substantially free of polyadenylated RNA in a collection vial at the discharge of the column.
Claim 5
Dependent Claim
91 words
A chromatography method for separating polyadenylated RNA from nonpolyadenylated RNA, said method comprising the steps of: providing a column with a chromatography material including oligo(dT)-cellulose; introducing into the column a sample of polyadenylated RNA and nonpolyadenylated RNA; attaching a syringe to one end of the column; using the syringe to induce a pneumatic pressure differential across said column sufficient to cause said sample to pass through said chromatography material; and collecting eluent containing said nonpolyadenylated RNA substantially free of polyadenylated RNA in a collection vial at the discharge of the column.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-08-08 | 1998-02-07 | 1998-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-08-08 | 2002-02-07 | 2002-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-08-08 | 2006-02-07 | 2006-08-09 | $7700.00 | Window Closed / Lapsed |