Patent US5328844A successfully refreshed from Google Patents.
US5328844A
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Culture media for mammalian cells
Assignee: University of Colorado Foundation Inc
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Filing: 1992-06-24
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Granted: 1994-07-12
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Expires: 2012-06-24
Abstract
Abstract The present invention provides a culture medium designated COF 1769 useful for establishing, growing and maintaining mammalian cells in culture, in particular for the establishment and culture of human normal and malignant cells. Also provided by the invention is an improved method for obtaining growth of mammalian cells.
Inventors
- 👤 George E. Moore
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:46
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; C12N2500/00; C12N2500/30; C12N2500/44; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/30; C12N2501/38; C12N2501/39; C12N2501/395
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
25 words
A culture medium for establishing, growing and maintaining mammalian cells consisting essentially of the composition of the composition COF 1769 as defined in Table 1.
Claim 2
Dependent Claim
25 words
A culture medium for establishing, growing and maintaining mammalian cells consisting essentially of the composition of the composition COF 1769 as defined in Table 1.
Claim 3
Dependent Claim
18 words
The medium according to claim 1 which further includes serum at a level not greater than about 2.5%.
Claim 4
Dependent Claim
22 words
The medium according to claim 1 which further includes serum at a level greater than 2.5% but not greater than about 20%.
Claim 5
Dependent Claim
28 words
A culture medium for establishing, growing and maintaining mammalian cells consisting essentially of the composition COF 1769 as defined in Table 1, but without epidermal growth factor (EGF).
Claim 6
Dependent Claim
28 words
A culture medium for establishing, growing and maintaining mammalian cells consisting essentially of the composition COF 1769 as defined in Table 1, but without epidermal growth factor (EGF).
Claim 7
Dependent Claim
18 words
The medium according to claim 4 which further includes serum at a level not greater than about 2.5%.
Claim 8
Dependent Claim
22 words
The medium according to claim 4 which further includes serum at a level greater than 2.5% but not greater than about 20%.
Claim 9
Dependent Claim
43 words
A culture medium for establishing, growing and maintaining mammalian cells consisting essentially of the composition COF 1769 as defined in Table 1 for the establishment, growth and maintenance of cells selected from the group consisting of human melanoma cells and human adenocarcinoma cells.
Claim 10
Dependent Claim
43 words
A culture medium for establishing, growing and maintaining mammalian cells consisting essentially of the composition COF 1769 as defined in Table 1 for the establishment, growth and maintenance of cells selected from the group consisting of human melanoma cells and human adenocarcinoma cells.
Claim 11
Dependent Claim
13 words
The medium of claim 7 for establishment, growth and maintenance of melanoma cells.
Claim 12
Dependent Claim
13 words
The medium of claim 7 for establishment, growth and maintenance of adenocarcinoma cells.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-07-11 | 1998-01-10 | 1998-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-07-11 | 2002-01-10 | 2002-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-07-11 | 2006-01-10 | 2006-07-12 | $7700.00 | Window Closed / Lapsed |