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US5326703A
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Method of degrading pollutants in soil
Assignee: United States Department of Energy
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Filing: 1992-06-10
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Granted: 1994-07-05
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Expires: 2012-06-10
Abstract
Abstract A method and system for enhancing the motility of microorganisms by placing an effective amount of chlorinated hydrocarbons, preferably chlorinated alkenes, and most preferably trichloroethylene in spaced relation to the microbes so that the surprisingly strong, monomodal, chemotactic response of the chlorinated hydrocarbon on subsurface microbes can draw the microbes away from or towards and into a substance, as desired. In remediation of groundwater pollution, for example, TCE can be injected into the plume to increase the population of microbes at the plume whereby the plume can be more quickly degraded. A TCE-degrading microbe, such as Welchia alkenophilia, can be used to degrade the TCE following the degradation of the original pollutant.
Inventors
- 👤 Terry C. Hazen
- 👤 Geralyne Lopez-De-Victoria
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 20:07
CPC Classifications
C; C02; C02F; C02F3/00; C02F3/02; C02F3/12; C02F3/1205; C02F3/1231; B; B09; B09C; B09C1/00; B09C1/002; B09C1/10; C02F3/34; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N1/38; C12P; C12P1/00; C12P1/04; B09C2101/00; C12R; C12R2001/00; C12R2001/01; Y; Y02; Y02W; Y02W10/00; Y02W10/10; Y10; Y10S; Y10S435/00; Y10S435/821; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
78 words
A method of degrading a concentration of a pollutant in soil, said pollutant concentration being above a preselected level, said soil containing naturally occurring bacteria, said method comprising the steps of: placing a quantity of tetrachloroethylene in said pollutant whereby said bacteria are drawn to said tetrachloroethylene and whereby said bacteria begin to degrade said pollutant; monitoring said pollutant concentration; and removing said tetrachloroethylene from said pollutant when said pollutant concentration has been reduced to said preselected level.
Claim 2
Independent Claim
78 words
A method of degrading a concentration of a pollutant in soil, said pollutant concentration being above a preselected level, said soil containing naturally occurring bacteria, said method comprising the steps of: placing a quantity of tetrachloroethylene in said pollutant whereby said bacteria are drawn to said tetrachloroethylene and whereby said bacteria begin to degrade said pollutant; monitoring said pollutant concentration; and removing said tetrachloroethylene from said pollutant when said pollutant concentration has been reduced to said preselected level.
Claim 3
Dependent Claim
20 words
The method as recited in claim 1, wherein said tetrachloroethylene is removed by injecting a tetrachloroethylene-degrading bacteria into said tetrachloroethylene.
Claim 4
Dependent Claim
23 words
The method as recited in claim 1, wherein said tetrachloroethylene is removed by injecting Welchia alkenophilia into said tetrachloroethylene to degrade said tetrachloroethylene.
Claim 5
Dependent Claim
19 words
The method as recited in claim 1, wherein said quantity of tetrachloroethylene is between 1M and 10 -10 M.
Claim 6
Dependent Claim
24 words
The method as recited in claim 1, further comprising the step of injecting bacteria selected as known degraders of said pollutant near said pollutant.
Claim 7
Independent Claim
86 words
A method of degrading a concentration of a pollutant in soil, said pollutant concentration being above a preselected level, said soil containing naturally occurring bacteria, said method comprising the steps of: placing a quantity of tetrachloroethylene in said pollutant so that said bacteria are chemotactically attracted to said tetrachloroethylene and whereby said bacteria begin to degrade said pollutant; monitoring said pollutant concentration; and inoculating said tetrachloroethylene in said pollutant with bacteria known to degrade tetrachloroethylene when said pollutant concentration has been reduced to said preselected level.
Claim 8
Independent Claim
86 words
A method of degrading a concentration of a pollutant in soil, said pollutant concentration being above a preselected level, said soil containing naturally occurring bacteria, said method comprising the steps of: placing a quantity of tetrachloroethylene in said pollutant so that said bacteria are chemotactically attracted to said tetrachloroethylene and whereby said bacteria begin to degrade said pollutant; monitoring said pollutant concentration; and inoculating said tetrachloroethylene in said pollutant with bacteria known to degrade tetrachloroethylene when said pollutant concentration has been reduced to said preselected level.
Claim 9
Dependent Claim
21 words
The method as recited in claim 6, wherein said bacteria known to be degraders of tetrachloroethylene include the species Welchia alkenophilia.
Claim 10
Dependent Claim
19 words
The method as recited in claim 6, wherein said quantity of tetrachloroethylene is between 1M and 10 -10 M.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-07-04 | 1998-01-03 | 1998-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-07-04 | 2002-01-03 | 2002-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-07-04 | 2006-01-03 | 2006-07-05 | $7700.00 | Window Closed / Lapsed |