US5326356A
Expired / Lapsed
US
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Biocompatible perforated membranes, processes for their preparation, their use as a support in the in vitro growth of epithelial cells, the artificial skin obtained in this manner, and its use in skin grafts
Assignee: Fidia SpA
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Filing: 1993-02-16
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Granted: 1994-07-05
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Expires: 2013-02-16
Abstract
Abstract It is provided biocompatible membranes constructed of materials of natural, synthetic or semisynthetic origin, and having a thickness of between 10 and 500μ, characterised by containing an ordered series of holes of a constant size between 10 and 1000μ, separated from each other by a constant distance of between 50 and 1000μ, and obtained by perforation by mechanical, thermal laser or ultraviolet radiation means, they being suitable for use as a support for the in vitro growth of epithelial cells. Artificial skins obtained thereby and its use in grafts are also provided.
Inventors
- 👤 Francesco Della Valle
- 👤 Gabriella Calderini
- 👤 Alessandro Rastrelli
- 👤 Aurelio Romeo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 20:41
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0629; A; A61; A61F; A61F2/00; A61F2/02; A61F2/10; A61F2/105; A61L; A61L27/00; A61L27/50; A61L27/60; C12N5/0062; C12N5/0068; A61K; A61K35/00; A61K35/12; C12N2533/00; C12N2533/70; C12N2533/80; C12N2539/00; C12N2539/10; Y; Y10; Y10T; Y10T428/00; Y10T428/24; Y10T428/24273
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
68 words
Biocompatible membranes consisting of materials of natural, synthetic or semisynthetic original, and having a thickness of between 10 and 500μ, which comprise an ordered series of holes of a defined and constant size between 10 and 1000μ, separated from each other by a constant distance of between 50 and 1000μ, said membrane enabling in vitro culture of keratinocytes within the holes and on both sides of said membrane.
Claim 2
Dependent Claim
68 words
Biocompatible membranes consisting of materials of natural, synthetic or semisynthetic original, and having a thickness of between 10 and 500μ, which comprise an ordered series of holes of a defined and constant size between 10 and 1000μ, separated from each other by a constant distance of between 50 and 1000μ, said membrane enabling in vitro culture of keratinocytes within the holes and on both sides of said membrane.
Claim 3
Dependent Claim
17 words
Biocompatible membranes as claimed in claim 1, in which the hole size is between 40 and 70μ.
Claim 4
Dependent Claim
15 words
Biocompatible membranes as claimed in claim 1, in which the distance between holes is 80μ.
Claim 5
Dependent Claim
60 words
Biocompatible membranes as claimed in claim 1, in which the biocompatible material of natural origin is chosen from the group consisting of collagen or coprecipitates of collagen and glycosaminoglycans, cellulose, gelled polysaccharides and natural rubbers, either alone or in mixture with each other or with polymers of synthetic or semisynthetic origin, in the presence of suitable precipitating or gelling agents.
Claim 6
Dependent Claim
39 words
The biocompatible membranes according to claim 4 in which the gelled polysaccharides are selected from the group consisting of chitin, chitosan, pectins or pectic acids, agar, agarose, xanthan gum, gellano, alginic acid or alginates, polymannans or polyglucans and starches.
Claim 7
Dependent Claim
37 words
Biocompatible membranes as claimed in claim 1, in which the biocomcompatible material of synthetic origin is chosen from the group consisting of polylactic acid, polyglycolic acid or copolymers thereof or their derivatives, polydioxanones, polyphosphazenes, polysulphones and polyurethanes.
Claim 8
Dependent Claim
70 words
Biocompatible membranes as claimed in claim 1, in which the biocompatible material of semisynthetic origin is chosen from the group consisting of semisynthetic derivatives of natural polymers crosslinked with crosslinking agents and derivatives of cellulose, of alginic acid, of starch, of hyaluronic acid, of chitin or chitinosan, or gellan, of xanthan, of pectins or pectin acids, of polyglucans, of polymannans, of agar, of agarose, of natural rubbers or of glycosaminoglycans.
Claim 9
Dependent Claim
21 words
Biocompatible membranes as claimed in claim 7, in which the biocompatible membrane consists of hyaluronic acid benzyl ester with 100% esterification.
Claim 10
Dependent Claim
18 words
The biocompatible membranes according to claim 7 in which the semi-synthetic derivative of a natural polymer is collagen.
Claim 11
Dependent Claim
30 words
The biocompatible membranes according to claim 7 in which the crosslinking agent is selected from the group consisting of dialdehydes or their precursors, bicarboxylic acids or derivatives thereof and diamines.
Claim 12
Dependent Claim
13 words
The biocompatible membranes according to claim 1 which have a thickness of 20-40μ.
Claim 13
Dependent Claim
79 words
Artificial skin composed of a biocompatible support membrane consisting of materials of natural, synthetic or semisynthetic origin, and having a thickness of between 10 and 500μ, which contains an ordered series of holes of a defined and constant size between 10 and 1000μ, separated from each other by a constant distance of between 50 and 1000μ, together with autologous or heterologous keratinocyte microcolonies in the active proliferation stage present within said holes and on both sides of said membrane.
Claim 14
Dependent Claim
79 words
Artificial skin composed of a biocompatible support membrane consisting of materials of natural, synthetic or semisynthetic origin, and having a thickness of between 10 and 500μ, which contains an ordered series of holes of a defined and constant size between 10 and 1000μ, separated from each other by a constant distance of between 50 and 1000μ, together with autologous or heterologous keratinocyte microcolonies in the active proliferation stage present within said holes and on both sides of said membrane.
Claim 15
Dependent Claim
17 words
Artificial skin as claimed in claim 12, in which the hole size is between 40 and 70μ.
Claim 16
Dependent Claim
15 words
Artificial skin as claimed in claim 12, in which the distance between holes is 80μ.
Claim 17
Dependent Claim
60 words
Artificial skin as claimed in claim 12, in which the biocompatible material of natural origin is chosen from the group consisting of collagen or coprecipitates of collagen and glycosaminoglycans, cellulose, gelled polysaccharides and natural rubbers, either alone or in mixture with each other or with polymers of synthetic or semisynthetic origin, in the presence of suitable precipitating or gelling agents.
Claim 18
Dependent Claim
39 words
Artificial skin as claimed in claim 15 in which the gelled polysaccharides are selected from the group consisting of chitin, chitosan, pectins or petic acids, agar, agarose, xanthan gum, gellano, alginic acid or alginates, polymannans or polyglucans and starches.
Claim 19
Dependent Claim
37 words
Artificial skin as claimed in claim 12, in which the biocompatible material of synthetic origin is chosen from the group consisting of polylactic acid, polyglycolic acid or copolymers thereof or their derivatives, polydioxanones, polyphosphazenes, polysulphones and polyurethanes.
Claim 20
Dependent Claim
70 words
Artificial skin as claimed in claim 12, in which the biocompatible material of semisynthetic origin is chosen from the group consisting of semisynthetic derivatives of natural polymers crosslinked with crosslinking agents and derivatives of cellulose, of alginic acid, of starch, of hyaluronic acid, of chitin or chitosan, of gellano, of xanthan, of pectins or pectic acids, of polyglucans, of polymannans, of agar, of agarose, of natural rubbers or of glycosaminoglycans.
Claim 21
Dependent Claim
21 words
Artificial skin as claimed in claim 18, in which the biocompatible material consists of hyaluronic acid benzyl ester with 100% esterification.
Claim 22
Dependent Claim
26 words
Artificial skin according to claim 19 which has a thickness of 20-40μ and the holes are separated from each other by a constant distance of 80μ.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-07-04 | 1998-01-03 | 1998-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-07-04 | 2002-01-03 | 2002-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-07-04 | 2006-01-03 | 2006-07-05 | $7700.00 | Window Closed / Lapsed |