Taxol production by taxomyces andreanae

Claim 2

A biologically pure culture of the fungus Taxomyces andreanae having all the identifying characteristics of Taxomyces andreanae CBS 279.92.

Claim 3

The biologically pure culture of claim 1, which is isolated from a tree of the genus Taxus.

Claim 4

The biologically pure culture of claim 1, which is Taxomyces andreanae CBS 279.92.

Claim 5

The biologically pure culture of claim 1, wherein said fungus shows positive α-action antitubulin activity in a brine-shrimp assay.

Claim 6

The biologically pure culture of claim 1, wherein said fungus has a hyphae which average 1.2 μm in diameter and have bulbi which are incapable of germination.

Claim 7

The biologically pure culture of claim 1, wherein said fungus has superficial mycelium composed of a network of highly branched, septate, hyaline, smooth walled hyphae which average 1.25 μm in diameter, and larger cells which average 3.75 μm in diameter, wherein said mycelium are budded from fructigenous hyphae forming clumps which vary in shape from spherical, to ovoid, to longiform and in size from 5×5 μm in length, wherein said hyphae possess bulbil cells which lack pigmentation, are loosely packed in the bulbil, are ovoid, have a size of 1.5×2.5 μm and do not germinate.

Claim 8

The biologically pure culture of claim 2 produced by a process, which comprises, (a) obtaining tissue fragments from a tree of the Genus Taxus; (b) placing said tissue fragments on agar medium until fungal growth occurs; (c) placing fungal hyphae from said fungal growth on mycological agar until a culture in pure form is obtained; (d) transferring said fungal hyphae to a fungal lab growth medium, and growing a fungal culture; (e) removing at least a portion of the culture media containing the fungal culture, thoroughly grinding the mycelium, and adding a chromatographic solvent to the mixture; (f) obtaining a chromotograph of said fungal culture in said solvent, ad (g) checking the solution for the taxane reaction with vanillin-sulfuric spray, and comparing the chromatograph with one or more taxane standards selected from the group consisting of taxol, baccatin and cephalomannine.

Claim 9

The biologically pure culture of claim 2, wherein said tree is a species selected from the group consisting of Taxus brevifolia, taxus baccata, Taxus cuspiduta, Taxus canadensis, and Taxus floridana.

Claim 10

A process for producing taxol comprising the steps of a) culturing a fungus having all the identifying characteristics of Taxomyces andreanae CBS 279.92 in a medium suitable for its growth and b) recovering the taxol produced.

Claim 11

A process for producing taxol comprising the steps of a) culturing a fungus having all the identifying characteristics of Taxomyces andreanae CBS 279.92 in a medium suitable for its growth and b) recovering the taxol produced.