US5312921A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Dyes designed for high sensitivity detection of double-stranded DNA
Assignee: University of California
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Filing: 1993-05-14
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Granted: 1994-05-17
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Expires: 2013-05-14
Abstract
Abstract Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Inventors
- 👤 Alexander N. Glazer
- 👤 Scott C. Benson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 02:13
CPC Classifications
C; C07; C07D; C07D417/00; C07D417/02; C07D417/06; C07D221/00; C07D221/02; C07D221/04; C07D221/06; C07D221/10; C07D221/12; C07D405/00; C07D405/02; C07D405/12; C07D417/14; C09; C09B; C09B23/00; C09B23/02; C09B23/04; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01N27/44726
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
4 words
Thiazole orange tetramethylpropane diamine.
Claim 2
Dependent Claim
4 words
Thiazole orange tetramethylpropane diamine.
Claim 3
Dependent Claim
4 words
Thiazole orange tetramethyl diamine.
Claim 4
Dependent Claim
4 words
Thiazole orange tetramethyl diamine.
Claim 5
Dependent Claim
3 words
Ethidium propane diamine.
Claim 6
Dependent Claim
3 words
Ethidium propane diamine.
Claim 7
Dependent Claim
3 words
Ethidium diethylene triamine.
Claim 8
Dependent Claim
3 words
Ethidium diethylene triamine.
References Cited (Backward Citations)
US4883867A
Cited
US4937198A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-05-16 | 1997-11-15 | 1998-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-05-16 | 2001-11-15 | 2002-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-05-16 | 2005-11-15 | 2006-05-17 | $7700.00 | Window Closed / Lapsed |