US4937198A
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Novel fluorescent dye
Assignee: Becton Dickinson and Co
•
Filing: 1989-07-28
•
Granted: 1990-06-26
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Expires: 2009-07-28
Abstract
Abstract A novel fluorescent dye, PUR-1, having the structural formula ##STR1## is disclosed that will preferentially stain nucleic acids.
Inventors
- 👤 Linda G. Lee
- 👤 Patrick D. Mize
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 21:59
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C07; C07D; C07D473/00; C09; C09B; C09B23/00; C09B23/02; C09B23/04; C12Q2304/00; C12Q2304/10; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
33 words
A compound having the formula: ##STR7## where R is selected from the group consisting of CH 3 and CH 2 COO - , X - is an anion and n is an integer.
Claim 2
Dependent Claim
33 words
A compound having the formula: ##STR7## where R is selected from the group consisting of CH 3 and CH 2 COO - , X - is an anion and n is an integer.
Claim 3
Dependent Claim
11 words
The compound of claim 1 wherein R is CH 3 .
Claim 4
Dependent Claim
13 words
The compound of claim 1 wherein R is CH 2 COO - .
Claim 5
Dependent Claim
19 words
The compound of claim 1 wherein the anion is selected from the group consisting of acetate, glucose-6-phosphate and D-glucuronate.
Claim 6
Dependent Claim
10 words
The compound of claim 4 wherein X - is D-glucuronate.
Claim 7
Dependent Claim
11 words
The compound of claim 1 wherein said anion is a halide.
Claim 8
Dependent Claim
12 words
The compound of claim 6 wherein X - is I - .
Claim 9
Dependent Claim
39 words
The compound of claim 1 wherein said anion is selected from the group consisting of PO 4 3- , SO 4 2- , IO 4 - , ClO 4 - , NO 3 - and NO 2 - .
Claim 10
Dependent Claim
13 words
The compound of claim 8 wherein X - is PO 4 3- .
Claim 11
Dependent Claim
13 words
The compound of claim 8 wherein X - is SO 4 2- .
Claim 12
Dependent Claim
20 words
A method of staining nucleic acids in a biological sample comprising contacting said sample with a compound of claim 1.
Claim 13
Dependent Claim
14 words
The method of claim 11 wherein the sample is blood or a component thereof.
Claim 14
Dependent Claim
11 words
The method of claim 11 wherein the nucleic acid is RNA.
Claim 15
Dependent Claim
11 words
The method of claim 11 wherein the nucleic acid is DNA.
Claim 16
Dependent Claim
6 words
A compound having the formula: ##STR8##
Claim 17
Dependent Claim
6 words
A compound having the formula: ##STR8##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-06-25 | 1993-12-25 | 1994-06-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-06-25 | 1997-12-25 | 1998-06-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-06-25 | 2001-12-25 | 2002-06-26 | $7700.00 | Window Closed / Lapsed |