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US5298741A Expired / Lapsed US ⏱️ 0.0 years remaining

Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample

Assignee: Tufts University
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Filing: 1993-01-13
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Granted: 1994-03-29
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Expires: 2013-01-13
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Abstract

Abstract The present invention provides a unique fiber optic sensor which is able to provide a visual examination of a sample and its surrounding environment concurrent with the ability to conduct chemical sensing and detection of at least one ligand of interest. The present invention also provides apparatus for making precise optical determinations and measurements for the ligand of interest which can be correlated with specific parameter or other ligands for specific applications and purposes.

Inventors

  • 👤 David R. Walt
  • 👤 Karen S. Bronk

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-30 18:51
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N21/77; G01N21/7703; A; A61; A61B; A61B5/00; A61B5/0059; A61B5/0082; A61B5/0084; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G02; G02B; G02B6/00; G02B6/04; G02B6/06; A61B5/0071; A61B5/0086; G01N21/645; G01N2021/6463; G01N2021/6471; G01N2021/7793; G01N21/78; G01N21/80

Claims Analysis

Total Claims: 18
Claim 1
Independent Claim 173 words
A fiber optic sensor useful in an apparatus for concurrently viewing the environs of and optically detecting at least one ligand of interest of a fluid sample, said fiber optic sensor being comprised of: a preformed, unitary fiber optic array comprising a plurality of individually clad, fiber optical strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete ends of said preformed, unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy; and a substantially uniform and uninterrupted thin film comprising at least one light energy absorbing dye positioned over one of said discrete optic array surfaces of said preformed, unitary fiber optic array, said thin film having a thickness ranging from about 1-50 microns, each light energy absorbing dye of said thin film reacting with a ligand of interest in a manner correlatable with an optical determination.
Claim 2
Dependent Claim 173 words
A fiber optic sensor useful in an apparatus for concurrently viewing the environs of and optically detecting at least one ligand of interest of a fluid sample, said fiber optic sensor being comprised of: a preformed, unitary fiber optic array comprising a plurality of individually clad, fiber optical strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete ends of said preformed, unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy; and a substantially uniform and uninterrupted thin film comprising at least one light energy absorbing dye positioned over one of said discrete optic array surfaces of said preformed, unitary fiber optic array, said thin film having a thickness ranging from about 1-50 microns, each light energy absorbing dye of said thin film reacting with a ligand of interest in a manner correlatable with an optical determination.
Claim 3
Dependent Claim 29 words
The fiber optic sensor as recited in claim 1 further comprising a gradient index lens joined to and optically aligned with one end of said unitary fiber optic array.
Claim 4
Dependent Claim 19 words
The fiber optic sensor as recited in claim 1 wherein said thin film comprises at least two different dyes.
Claim 5
Dependent Claim 32 words
The fiber optic sensor as recited in claim 1 wherein the wavelength of said light energy absorbed by said dye is selected from the group consisting of infrared, visible and ultraviolet wavelengths.
Claim 6
Dependent Claim 27 words
The fiber optic sensor as recited in claim 1 wherein said dye is selected from the group consisting of fluorophores, fluorescent enzyme substrates and fluorescent antibody conjugates.
Claim 7
Dependent Claim 15 words
The fiber optic sensor as recited in claim 1 wherein said dye is a chromophore.
Claim 8
Independent Claim 273 words
An apparatus for concurrently viewing the environs of and optically detecting at least one ligand of interest of a fluid sample, said apparatus comprising: a fiber optic sensor comprised of (a) a preformed, unitary fiber optic array comprising a plurality of individually clad, fiber optical strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete ends of said unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy, (b) a substantially uniform and uninterrupted thin film comprising at least one light energy absorbing dye positioned over one of said discrete optic array surfaces of said preformed, unitary fiber optic array, said thin film having a thickness ranging from about 1-50 microns, each light energy absorbing dye of said thin film reacting with one ligand of interest in a manner correlatable with an optical determination; means for placing said thin film of said fiber optic sensor into reactive contact with a fluid sample; means for introducing light energy to said thin film of said fiber optic sensor for illumination of said light energy absorbing dye; means for detecting emerging light energy from said illuminated thin film of said fiber optic sensor, said detected emerging light energy serving as an optical determination for a ligand of interest in the fluid sample; means for illuminating the fluid sample and its environs; and means for concurrently viewing the fluid sample and its environs through said thin film of said fiber optic sensor.
Claim 9
Independent Claim 273 words
An apparatus for concurrently viewing the environs of and optically detecting at least one ligand of interest of a fluid sample, said apparatus comprising: a fiber optic sensor comprised of (a) a preformed, unitary fiber optic array comprising a plurality of individually clad, fiber optical strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete ends of said unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy, (b) a substantially uniform and uninterrupted thin film comprising at least one light energy absorbing dye positioned over one of said discrete optic array surfaces of said preformed, unitary fiber optic array, said thin film having a thickness ranging from about 1-50 microns, each light energy absorbing dye of said thin film reacting with one ligand of interest in a manner correlatable with an optical determination; means for placing said thin film of said fiber optic sensor into reactive contact with a fluid sample; means for introducing light energy to said thin film of said fiber optic sensor for illumination of said light energy absorbing dye; means for detecting emerging light energy from said illuminated thin film of said fiber optic sensor, said detected emerging light energy serving as an optical determination for a ligand of interest in the fluid sample; means for illuminating the fluid sample and its environs; and means for concurrently viewing the fluid sample and its environs through said thin film of said fiber optic sensor.
Claim 10
Dependent Claim 32 words
The apparatus as recited in claim 7 wherein said fiber optic sensor further comprises a gradient index lens joined to and optically aligned with one end of said unitary fiber optic array.
Claim 11
Dependent Claim 20 words
The apparatus as recited in claim 7 further comprising automated means for concurrently introducing light energy and detecting emerging light.
Claim 12
Dependent Claim 18 words
The apparatus as recited in claim 7 wherein said detected emerging light is visualized as a discrete image.
Claim 13
Dependent Claim 17 words
The apparatus as recited in claim 7 further comprising a computer controlled imaging and data processing system.
Claim 14
Independent Claim 268 words
A method for concurrently viewing the environs of and optically detecting at least one ligand of interest of a fluid sample, and method comprising the steps of: obtaining a fiber optic sensor comprised of (a) a preformed, unitary fiber optic array comprising a plurality of individually clad, fiber optical strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete ends of said preformed, unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy, (b) a substantially uniform and uninterrupted thin film comprising at least one light energy absorbing dye and positioned over one of said discrete optic array surfaces of said preformed, unitary fiber optic array, said thin film having a thickness ranging from about 1-50 microns, each light energy absorbing dye of said thin film reacting with one ligand of interest in a manner correlatable with an optical determination; placing said thin film of said fiber optic sensor into reactive contact with a fluid sample; introducing light energy to said thin film of said fiber optic sensor for illumination of said light energy absorbing dye; detecting emerging light energy from said thin film of said fiber optic sensor, said detected emerging light energy serving as an optical determination for a ligand of interest in the fluid sample; illuminating the fluid sample and its environs; and concurrently viewing the fluid sample and its environs through said thin film of said fiber optic sensor.
Claim 15
Independent Claim 268 words
A method for concurrently viewing the environs of and optically detecting at least one ligand of interest of a fluid sample, and method comprising the steps of: obtaining a fiber optic sensor comprised of (a) a preformed, unitary fiber optic array comprising a plurality of individually clad, fiber optical strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete ends of said preformed, unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy, (b) a substantially uniform and uninterrupted thin film comprising at least one light energy absorbing dye and positioned over one of said discrete optic array surfaces of said preformed, unitary fiber optic array, said thin film having a thickness ranging from about 1-50 microns, each light energy absorbing dye of said thin film reacting with one ligand of interest in a manner correlatable with an optical determination; placing said thin film of said fiber optic sensor into reactive contact with a fluid sample; introducing light energy to said thin film of said fiber optic sensor for illumination of said light energy absorbing dye; detecting emerging light energy from said thin film of said fiber optic sensor, said detected emerging light energy serving as an optical determination for a ligand of interest in the fluid sample; illuminating the fluid sample and its environs; and concurrently viewing the fluid sample and its environs through said thin film of said fiber optic sensor.
Claim 16
Dependent Claim 15 words
The method as recited in claim 12 wherein said detecting and viewing is made in-vivo.
Claim 17
Dependent Claim 15 words
The method as recited in claim 12 wherein said detecting and viewing is made in-vitro.
Claim 18
Dependent Claim 22 words
The method as recited in claim 12 wherein said detecting and viewing is made using an automated imaging and data processing system.

References Cited (Backward Citations)

USRE31879E Cited
US4144452A Cited
US4495293A Cited
US4495293B1 Cited
US4822746A Cited
US5244636A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1997-03-28 1997-09-27 1998-03-29 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2001-03-28 2001-09-27 2002-03-29 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2005-03-28 2005-09-27 2006-03-29 $7700.00 Window Closed / Lapsed

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