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US5290693A
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Immobilization of microorganisms or enzymes in polyvinyl alcohol beads
Assignee: National Science and Technology Council
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Filing: 1992-07-08
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Granted: 1994-03-01
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Expires: 2012-07-08
Abstract
Abstract Microorganisms or enzymes are immobilized by using polyvinyl alcohol to form beads containing a microorganism or an enzyme. An aqueous solution of about 10 to about 20 wt % polyvinyl alcohol containing a microorganism or an enzyme is reacted with an aqueous solution of about 3 wt % to saturated boric acid for a period of about 10 minutes to about two hours to form gelled spherical beads. The beads are then hardened by treatment with an aqueous solution of about 3 to about 20 wt % phosphoric acid or phosphate for at least 30 minutes. Preferably, the polyvinyl alcohol has a degree of polymerization of about 1000 to about 3000 and a degree of saponification of about 70 to about 99 mol %. In an alternative embodiment, the boric acid solution can contain the phosphoric acid or phosphate. The microorganism can be an acclimatized activated sludge microorganism from agricultural or industrial waste water. The beads can be used for removing inorganic nitrogen and organic carbon in waste water treatment or in processes for making biochemical products.
Inventors
- 👤 Kuo-Cheng Chen
- 👤 Ying-Feng Lin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:43
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12N11/18
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
134 words
A process for preparing polyvinyl alcohol-immobilized microbial or enzymatic beads, said process comprising the steps of: reacting about a 10 to about a 20 wt % aqueous solution of polyvinyl alcohol and a microorganism or an enzyme with about 3 wt % to saturated boric acid aqueous solution for a period of about 10 minutes to about two hours to form gelled spherical beads, wherein said polyvinyl alcohol has a degree of polymerization from about 1000 to about 3000 and a degree of saponification from about 70 to about 99 mol %; removing said gelled spherical beads from said reaction mixture; and hardening said gelled spherical beads by treating said gelled spherical beads with about a 3 to about a 20 wt % phosphoric acid or phosphate aqueous solution for at least 30 minutes.
Claim 2
Dependent Claim
134 words
A process for preparing polyvinyl alcohol-immobilized microbial or enzymatic beads, said process comprising the steps of: reacting about a 10 to about a 20 wt % aqueous solution of polyvinyl alcohol and a microorganism or an enzyme with about 3 wt % to saturated boric acid aqueous solution for a period of about 10 minutes to about two hours to form gelled spherical beads, wherein said polyvinyl alcohol has a degree of polymerization from about 1000 to about 3000 and a degree of saponification from about 70 to about 99 mol %; removing said gelled spherical beads from said reaction mixture; and hardening said gelled spherical beads by treating said gelled spherical beads with about a 3 to about a 20 wt % phosphoric acid or phosphate aqueous solution for at least 30 minutes.
Claim 3
Dependent Claim
120 words
A process for preparing polyvinyl alcohol-immobilized microbial or enzymatic beads, said process comprising the steps of: reacting about a 10 to about a 20 wt % aqueous solution of polyvinyl alcohol and a microorganism or an enzyme with about 3 wt % to saturated boric acid aqueous solution for a period of about 30 minutes to about three hours to form gelled spherical beads, wherein said boric acid aqueous solution further comprises about 3 to about 20 wt % phosphoric acid or phosphate and said polyvinyl alcohol has a degree of polymerization from about 1000 to about 3000 and a degree of saponification from about 70 to about 99 mol %; and recovering said spherical beads from said aqueous solution.
Claim 4
Dependent Claim
120 words
A process for preparing polyvinyl alcohol-immobilized microbial or enzymatic beads, said process comprising the steps of: reacting about a 10 to about a 20 wt % aqueous solution of polyvinyl alcohol and a microorganism or an enzyme with about 3 wt % to saturated boric acid aqueous solution for a period of about 30 minutes to about three hours to form gelled spherical beads, wherein said boric acid aqueous solution further comprises about 3 to about 20 wt % phosphoric acid or phosphate and said polyvinyl alcohol has a degree of polymerization from about 1000 to about 3000 and a degree of saponification from about 70 to about 99 mol %; and recovering said spherical beads from said aqueous solution.
Claim 5
Dependent Claim
42 words
The process of claim 1 wherein said boric acid aqueous solution is a saturated boric acid aqueous solution, and said mixture of polyvinyl alcohol and microorganism or enzyme is treated with said boric acid aqueous solution for a period of 15-30 minutes.
Claim 6
Dependent Claim
39 words
The process of claim 1 wherein said phosphoric acid or said phosphate aqueous solution has a concentration of 5-15% by weight and said gelled spherical beads are hardened in said phosphoric acid or phosphate aqueous solution for 1-2 hours.
Claim 7
Dependent Claim
24 words
The process of claim 2 wherein said boric acid aqueous solution contains saturated boric acid and 5-15 wt % of phosphoric acid or phosphate.
Claim 8
Dependent Claim
11 words
The process of claim 2 wherein said period is 1-2 hours.
Claim 9
Dependent Claim
25 words
The process of claim 1 wherein said phosphate is selected from the group consisting of sodium phosphate, potassium phosphate, ammonium phosphate, and acid salts thereof.
Claim 10
Dependent Claim
25 words
The process of claim 2 wherein said phosphate is selected from the group consisting of sodium phosphate, potassium phosphate, ammonium phosphate, and acid salts thereof.
Claim 11
Dependent Claim
28 words
The process of claim 1 wherein said polyvinyl alcohol has a degree of polymerization from about 1500 to about 2000 and a 95-99 mol % degree of saponification.
Claim 12
Dependent Claim
23 words
The process of claim 2 wherein said polyvinyl alcohol has a 1500-2000 degree of polymerization and a 95-99 mol % degree of saponification.
Claim 13
Dependent Claim
14 words
The process of claim 1 wherein said microorganism is bacteria, fungi, algae, or protozoda.
Claim 14
Dependent Claim
14 words
The process of claim 2 wherein said microorganism is bacteria, fungi, algae, or protozoda.
Claim 15
Dependent Claim
20 words
The process of claim 1 wherein said microorganism is an acclimatized activated sludge microorganism of agricultural or industrial waste water.
Claim 16
Dependent Claim
20 words
The process of claim 2 wherein said microorganism is an acclimatized activated sludge microorganism of agricultural or industrial waste water.
Claim 17
Dependent Claim
13 words
The process of claim 1 wherein said enzyme is amylase, cellulase, or proteinase.
Claim 18
Dependent Claim
13 words
The process of claim 2 wherein said enzyme is amylase, cellulase, or proteinase.
References Cited (Backward Citations)
JPS645490A
Cited
JPS645491A
Cited
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