Abstract A method and probes for specifically and sensitively detecting, identifying, and quantitating any organism category or group of organisms containing RNA in a sample is disclosed. The nucleic acids of the organisms present in the sample are brought together with a marked probe comprising nucleic acid molecules which are complementary only to nucleic acid containing sequences complementary to RNA sequences known to be conserved in an organism, category or group of organisms. The probe in sample nucleic acid mixture is incubated under nucleic acid hybridization conditions and then assayed to determine the degree of hybridization that has occurred. Hybridization indicates the presence and identity of the organism, category or group of organisms in the sample. The quantity of RNA present in the sample can be determined and compared to that normally present in the known organisms to determine the number of organisms present. Batteries of sequentially more specific probes can also be utilized.
A method for detecting the presence of a species of organism comprising a ribosomal nucleic acid sequence, in a test sample, comprising the steps of: contacting ribosomal nucleic acid from said test sample with a nucleic acid probe able to hybridize to only a portion of said ribosomal nucleic acid sequence of said organism, incubating said probe and said ribosomal nucleic acid obtained from said test sample under specified hybridization conditions such that said probe hybridizes to the ribosomal nucleic acid of said organism and does not detectably hybridize to ribosomal nucleic acid from other species and detecting any hybridization of said probe with said ribosomal nucleic acid in said test sample as an indication of the presence of said organism comprising said ribosomal nucleic acid sequence in said sample.
Claim 2
Independent Claim
130 words
A method for detecting the presence of a species of organism comprising a ribosomal nucleic acid sequence, in a test sample, comprising the steps of: contacting ribosomal nucleic acid from said test sample with a nucleic acid probe able to hybridize to only a portion of said ribosomal nucleic acid sequence of said organism, incubating said probe and said ribosomal nucleic acid obtained from said test sample under specified hybridization conditions such that said probe hybridizes to the ribosomal nucleic acid of said organism and does not detectably hybridize to ribosomal nucleic acid from other species and detecting any hybridization of said probe with said ribosomal nucleic acid in said test sample as an indication of the presence of said organism comprising said ribosomal nucleic acid sequence in said sample.