US5284761A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of encapsulating cells in a tubular extrudate
Assignee: Brown University Research Foundation Inc
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Filing: 1992-02-26
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Granted: 1994-02-08
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Expires: 2012-02-26
Abstract
Abstract Methods and systems are disclosed for encapsulating viable cells which produce biologically-active factors. The cells are encapsulated within a semipermeable, polymeric membrane by co-extruding an aqueous cell suspension and a polymeric solution through a common port to form a tubular extrudate having a polymeric outer coating which encapsulates the cell suspension. For example, the cell suspension and the polymeric solution can be extruded through a common extrusion port having at least two concentric bores, such that the cell suspension is extruded through the inner bore and the polymeric solution is extruded through the outer bore. The polymeric solution coagulates to form an outer coating. As the outer coating is formed, the ends of the tubular extrudate can be sealed to form a cell capsule. In one embodiment, the tubular extrudate is sealed at intervals to define separate cell compartments connected by polymeric links.
Inventors
- 👤 Patrick Aebischer
- 👤 Lars Wahlberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:14
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/0087; A61K9/0092; A61F; A61F2/00; A61F2/02; A61F2/022; A61K35/00; A61K35/12; A61K9/0012; A61K9/0019; A61K9/0024; A61K9/0085; A61K9/48; A61K9/4816; A61K9/4833; A61M; A61M31/00; A61M31/002; B; B01; B01J; B01J13/00; B01J13/02; B01J13/04; C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12N11/08; C12N11/082; C12N11/087; C12N11/098; C12N5/00; C12N5/0012; C12N5/06; C12N5/0602; C12N5/0613; C12N5/0614; C12N5/0618; C12N5/0619; A61K2035/126; A61K2035/128; A61M2210/00; A61M2210/06; A61M2210/0687; A61M2210/0693; C12N2533/00; C12N2533/30
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
137 words
A method of encapsulating viable cells comprising (i) co-extruding an aqueous cell suspension and a polymeric solution through a common extrusion port having at least two concentric bores to form a tubular extrudate having a polymeric outer coating which encapsulates said cell suspension, wherein said cell suspension is extruded though an inner bore and said polymeric solution is extruded though an outer bore and a pressure differential is maintained between said cell suspension and said polymeric solution during co-extrusion to impede solvent diffusion from said polymeric solution into said cell suspension and said polymeric solution and said cell suspension are chosen so that coagulation of said polymeric solution occurs as the polymeric solution and cell suspension are extruded through the extrusion port; and (ii) sealing said tubular extrudate to form at least one isolated tubular cell compartment.
Claim 2
Independent Claim
137 words
A method of encapsulating viable cells comprising (i) co-extruding an aqueous cell suspension and a polymeric solution through a common extrusion port having at least two concentric bores to form a tubular extrudate having a polymeric outer coating which encapsulates said cell suspension, wherein said cell suspension is extruded though an inner bore and said polymeric solution is extruded though an outer bore and a pressure differential is maintained between said cell suspension and said polymeric solution during co-extrusion to impede solvent diffusion from said polymeric solution into said cell suspension and said polymeric solution and said cell suspension are chosen so that coagulation of said polymeric solution occurs as the polymeric solution and cell suspension are extruded through the extrusion port; and (ii) sealing said tubular extrudate to form at least one isolated tubular cell compartment.
Claim 3
Dependent Claim
25 words
The method of claim 1 wherein the method further includes sealing the tubular extrudate at intervals to define separate cell compartments connected by polymeric links.
Claim 4
Dependent Claim
24 words
The method of claim 2 wherein the step of sealing the extrudate further comprises compressing the extrudate at intervals to define separate cell compartments.
Claim 5
Dependent Claim
40 words
The method of claim 2 wherein the step of sealing the extrudate further comprises modifying the pressure under which the cell suspension or the polymeric solution is extruded, thereby collapsing the tubular extrudate at intervals to define separate cell compartments.
Claim 6
Dependent Claim
33 words
The method of claim 2 wherein the step of sealing the extrudate further comprises impeding the flow of the cell suspension at intervals to collapse the tubular extrudate and define separate cell compartments.
Claim 7
Dependent Claim
14 words
The method of claim 1 wherein the extrusion port is in contact with air.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein the extrusion port is in contact with a quenchent.
Claim 9
Dependent Claim
16 words
The method of claim 1 wherein said cell suspension comprises cells that secrete a biologically-active factor.
Claim 10
Dependent Claim
15 words
The method of claim 1 wherein said cell suspension comprises cells that secrete a neurotransmitter.
Claim 11
Dependent Claim
14 words
The method of claim 1 wherein said cell suspension further comprises a nutrient medium.
Claim 12
Dependent Claim
15 words
The method of claim 1 wherein said cell suspension further comprises an anchorage substrate material.
Claim 13
Dependent Claim
15 words
The method of claim 1 wherein said polymeric solution further comprises a water-miscible solvent component.
Claim 14
Dependent Claim
13 words
The method of claim 1 wherein said polymeric solution further comprises a surfactant.
Claim 15
Dependent Claim
14 words
The method of claim 1 wherein said polymeric solution further comprises an anti-inflamatory agent.
Claim 16
Dependent Claim
13 words
The method of claim 1 wherein said polymeric solution further comprises an antioxidant.
Claim 17
Dependent Claim
34 words
The method of claim 1 wherein the method further comprises controlling the viscosity of said polymeric solution, such that upon coagulation said outer polymeric coating will form a semipermeable membrane about said cell suspension.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-02-07 | 1997-08-09 | 1998-02-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-02-07 | 2001-08-09 | 2002-02-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-02-07 | 2005-08-09 | 2006-02-08 | $7700.00 | Window Closed / Lapsed |