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US5279969A
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Method of identifying and modulating the activity of label retaining cells in hair follicles for diagnostic and therapeutic purposes
Assignee: New York University
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Filing: 1992-11-04
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Granted: 1994-01-18
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Expires: 2012-11-04
Abstract
Abstract Methods of detecting label retaining cells in self-renewing tissues, such as hair follicle, are provided. Methods of modulating the activity of slow-cycling cells and methods of evaluating the efficacy of agents for modulating the activity of stem cell populations are also provided. Generally, cells are continuously labeled, induced into a proliferative phase by exposure to an agent such as TPA or a cytokine, and then observed in relation to established criteria.
Inventors
- 👤 Robert M. Lavker
- 👤 Tung-Tien Sun
- 👤 George Cotsarelis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 18:49
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0627; C12N5/0628; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; C12N2500/00; C12N2500/30; C12N2500/40; C12N2501/00; C12N2501/01; C12N2501/10; C12N2501/11; C12N2501/30; C12N2501/33; C12N2501/38; C12N2501/39; C12N2501/70; C12N2501/998; C12N2503/00; C12N2503/02
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
93 words
Method of evaluating the efficacy of agents for modulating the activity of bulge cell populations comprising: (a) collecting bulge cells from the midportion of a hair follicle at the arrector pili muscle attachment site; (b) exposing said bulge cells to an agent to be evaluated; (c) continuously labeling said exposed bulge cells with a selected radioisotope for a selected period; (d) inducing said labeled bulge cells into a proliferative phase; (e) observing said bulge cells for labeling; and (f) comparing the labeling of said bulge cells with established controls for labeled bulge cells.
Claim 2
Dependent Claim
93 words
Method of evaluating the efficacy of agents for modulating the activity of bulge cell populations comprising: (a) collecting bulge cells from the midportion of a hair follicle at the arrector pili muscle attachment site; (b) exposing said bulge cells to an agent to be evaluated; (c) continuously labeling said exposed bulge cells with a selected radioisotope for a selected period; (d) inducing said labeled bulge cells into a proliferative phase; (e) observing said bulge cells for labeling; and (f) comparing the labeling of said bulge cells with established controls for labeled bulge cells.
Claim 3
Dependent Claim
26 words
The method of claim 1 wherein said labeled bulge cells are induced into a proliferative phase by exposing said bulge cells to TPA or a cytokine.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein said selected radioisotope is tritiated thymidine.
Claim 5
Dependent Claim
88 words
Method of evaluating bulge cells in a sample hair follicle for diagnostic and therapeutic purposes comprising: (a) collecting bulge cells from the midportion of said sample hair follicle at the arrector pili muscle attachment site; (b) continuously labeling said bulge cells for a selected period; (c) inducing said labeled bulge cells into a proliferative phase by exposure to TPA or a cytokine for a selected period; and (d) observing said bulge cells for labeling to determine the number and viability of said bulge cells relative to established controls.
Claim 6
Dependent Claim
88 words
Method of evaluating bulge cells in a sample hair follicle for diagnostic and therapeutic purposes comprising: (a) collecting bulge cells from the midportion of said sample hair follicle at the arrector pili muscle attachment site; (b) continuously labeling said bulge cells for a selected period; (c) inducing said labeled bulge cells into a proliferative phase by exposure to TPA or a cytokine for a selected period; and (d) observing said bulge cells for labeling to determine the number and viability of said bulge cells relative to established controls.
References Cited (Backward Citations)
US4806476A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-01-17 | 1997-07-19 | 1998-01-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-01-17 | 2001-07-19 | 2002-01-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-01-17 | 2005-07-19 | 2006-01-18 | $7700.00 | Window Closed / Lapsed |