Abstract Bifunctional chemicals useful for linking aldehyde containing moieties to moieties containing functionalities which are or can be reactive with sulfhydryl are claimed and have the formula: L--S--(CH.sub.2).sub.n1 --CONH--SPACER--NH--X wherein L is a leaving group selected from --H or --S--Ar, wherein Ar represents optionally substituted phenyl or pyridyl; n 1 =2-4; X is selected from: ##STR1## wherein Y is alkylene or oxaalkylene and Z is alkylene or a polypeptide residue briding the N-terminal amino group and C-terminal carboxy group thereof; the SPACER is oxaalkylene or oxaalkylene substituted with hydroxyl and specifically includes residues having formulas selected from --(CH.sub.2).sub.n2 --O--(CH.sub.2).sub.n2 --O--(CH.sub.2).sub.n2 --a) wherein each n2 is independently 2-4 and ##STR2## wherein n3 is 2-6. Also claimed are hydrazides, semicarbazides, and thiosemicarbazides of ω-mercaptocarboxylic acids which lack SPACER diamine.
A complex which comprises 1-{3-(2-pyridyldithio)propionamido}-12-{5-hydrazidoglutaramido}-4,9-dioxadodecane, bound through a disulfide linkage to a sulfhydryl-containing moiety.
Claim 2
Dependent Claim
14 words
A complex which comprises 1-{3-(2-pyridyldithio)propionamido}-12-{5-hydrazidoglutaramido}-4,9-dioxadodecane, bound through a disulfide linkage to a sulfhydryl-containing moiety.
Claim 3
Dependent Claim
65 words
A complex comprising a compound selected from those of the formula L--S--(CH 2 ) n1 CONHNH 2 , L--S--(CH 2 ) n1 NHCONHNH 2 , and L--S--(CH 2 ) n1 NHCSNHNH 2 , wherein L is a leaving group selected from --H or --S--Ar, wherein Ar represents optionally substituted phenyl or pyridyl, wherein n1 is 2-4, bound to a sulfhydryl-containing moiety through a disulfide linkage.
Claim 4
Dependent Claim
65 words
A complex comprising a compound selected from those of the formula L--S--(CH 2 ) n1 CONHNH 2 , L--S--(CH 2 ) n1 NHCONHNH 2 , and L--S--(CH 2 ) n1 NHCSNHNH 2 , wherein L is a leaving group selected from --H or --S--Ar, wherein Ar represents optionally substituted phenyl or pyridyl, wherein n1 is 2-4, bound to a sulfhydryl-containing moiety through a disulfide linkage.
Claim 5
Dependent Claim
12 words
The complex of claim 1, wherein the sulfhydryl-containing moiety is a protein.
Claim 6
Dependent Claim
12 words
The complex of claim 2, wherein the sulfhydryl-containing moiety is a protein.