Patent US5273902A successfully refreshed from Google Patents.
US5273902A
Expired / Lapsed
US
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Genetically modified vibrio
Assignee: University of South Florida
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Filing: 1991-10-31
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Granted: 1993-12-28
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Expires: 2011-10-31
Abstract
Abstract A high frequency of transformation strain of estuarine Vibrio species is derived from a parent strain naturally transformable with broad-host-range plasmids. The strain has a unique morphology and is generated from the parental strain by transformation by broad-host-range plasmid multimers, a cured of the plasmid by growth of plasmid transformants and nonselective medium resulting in the cured high frequency of transformation strain that transfer significantly more frequently than the parental strain.
Inventors
- 👤 Marc E. Frischer
- 👤 John H. Paul
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:53
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12R; C12R2001/00; C12R2001/01; C12R2001/63
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
105 words
A substantially pure form of high frequency of transformation strain of an estuarine Vibrio species selected from the group consisting of WJT-1C, MF-4C, and JT-1, said strain being derived from a parent strain naturally transformable with broad-host-range plasmids, said strain having a crenate colony morphology being rod shaped motile with single polar flagellum, oxidase positive and able to ferment glucose, said strain being generated from the parental Vibrio strain, transformed by broad-host-range plasmid multimers, and cured of said plasmid by growth of plasmid transformants in nonselective medium resulting in said cured high frequency of transformation strain that transform significantly more frequently than the parental strain.
Claim 2
Dependent Claim
105 words
A substantially pure form of high frequency of transformation strain of an estuarine Vibrio species selected from the group consisting of WJT-1C, MF-4C, and JT-1, said strain being derived from a parent strain naturally transformable with broad-host-range plasmids, said strain having a crenate colony morphology being rod shaped motile with single polar flagellum, oxidase positive and able to ferment glucose, said strain being generated from the parental Vibrio strain, transformed by broad-host-range plasmid multimers, and cured of said plasmid by growth of plasmid transformants in nonselective medium resulting in said cured high frequency of transformation strain that transform significantly more frequently than the parental strain.
Claim 3
Dependent Claim
19 words
A transformation strain as set forth in claim 1 wherein said plasmids are Inc P4 and MC P1 plasmids.
Claim 4
Dependent Claim
26 words
A transformation strain as set forth in claim 2 wherein said plasmids are selected from the group consisting of pGQ3, pQSR50, pKT230, R1162, pLV1013, and pAM120.
Claim 5
Dependent Claim
19 words
A transformation strain as set forth in claim 1 wherein said parent Vibrio strain is Vibrio sp. strain DI-9.
Claim 6
Dependent Claim
17 words
A transformation strain as set forth in claim 1 wherein said parent Vibrio strain is V. parahaemolyticus.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-12-27 | 1997-06-28 | 1997-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-12-27 | 2001-06-28 | 2001-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-12-27 | 2005-06-28 | 2005-12-28 | $7700.00 | Window Closed / Lapsed |