Patent US5266491A successfully refreshed from Google Patents.
US5266491A
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DNA fragment and expression plasmid containing the DNA fragment
Assignee: Mochida Pharmaceutical Co Ltd
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Filing: 1991-09-11
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Granted: 1993-11-30
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Expires: 2011-09-11
Abstract
Abstract Novel expression plasmids containing SV40 replication origin and a DNA fragment having a promoter region for a human polypeptide chain elongation factor-1α gene. The expression plasmids have high applicability to a wide range of host cells with high expression efficiency in transient expression systems.
Inventors
- 👤 Shigekazu Nagata
- 👤 Seiichi Mizushima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 04:34
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N2830/00; C12N2830/38; C12N2830/80; C12N2830/85
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
16 words
An essentially pure DNA fragment which is represented by the following sequence (SEQ ID NO:4): ##STR5##
Claim 2
Dependent Claim
16 words
An essentially pure DNA fragment which is represented by the following sequence (SEQ ID NO:4): ##STR5##
Claim 3
Dependent Claim
16 words
An essentially pure DNA fragment which is represented by the following sequence (SEQ ID NO:5): ##STR6##
Claim 4
Dependent Claim
16 words
An essentially pure DNA fragment which is represented by the following sequence (SEQ ID NO:5): ##STR6##
Claim 5
Dependent Claim
11 words
An expression plasmid containing the DNA fragment according to claim 1.
Claim 6
Dependent Claim
11 words
An expression plasmid containing a DNA fragment according to claim 2.
Claim 7
Dependent Claim
12 words
An expression plasmid according to claim 4, wherein the plasmid is pEF-BOS.
Claim 8
Dependent Claim
17 words
An expression plasmid containing a SV40 replication origin and at least one fragment according to claim 1.
Claim 9
Dependent Claim
17 words
An expression plasmid containing a SV40 replication origin and at least one fragment according to claim 2.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-29 | 1997-05-31 | 1997-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-29 | 2001-05-31 | 2001-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-29 | 2005-05-31 | 2005-11-30 | $7700.00 | Window Closed / Lapsed |