Patent US5260872A successfully refreshed from Google Patents.
US5260872A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Automated testing system
Assignee: Microbix Biosystems Inc
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Filing: 1991-10-04
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Granted: 1993-11-09
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Expires: 2011-10-04
Abstract
Abstract An automated testing system for the quality testing of production samples of a blood-clot dissolving product having (a) a frame structure having a horizontally disposed support surface; (b) a loading station for the placement of test tubes into a test tube rack; (c) a cooled pipetting station for automated dispensing and mixing of reagents and production samples; (d) a heated bead-drop station for simultaneous addition of a timing bead to each test tube, having a timer for measuring the time to completion of blood-clot dissolution by detecting the movement of each bead to the bottom of each test tube; (e) a robotic apparatus having an arm and pick-up and release device at the end thereof which is movable both vertically and horizontally and is adapted to move said test tube rack and tubes between said stations; and a programmable computer for controlling said dispensing, mixing and moving operations and recording the results of the product being tested.
Inventors
- 👤 Anne B. Copeland
- 👤 Danny R. Jackson
- 👤 Lucinda S. Oheim
- 👤 Margaret E. Stried
- 👤 Dennis D. Wagley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 01:26
CPC Classifications
G; G01; G01N; G01N35/00; G01N35/0099; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/4905; G01N35/00584; G01N35/00722; G01N35/00732; G01N2035/00742; G01N2035/00752; G01N35/10; G01N35/1065
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
313 words
An automated testing system for quality testing production samples of a blood-clot dissolving product comprising, a) a frame structure having a horizontally disposed support surface, b) a loading station on said support surface for receiving a test tube rack containing a plurality of test tubes, c) a pipetting station on said support surface characterized by an automatic computer-controlled pipetting apparatus and by reservoirs of buffer, media, plasma, fibrinogen, thrombin, and plasminogen solutions as well as production samples of the blood-clot dissolving product to be tested, all of which solutions are maintained at a certain cooling temperature of from 2°-8° C., which pipetting apparatus comprises means for providing vortex dispensing and mixing of said solutions and production samples in test tubes positioned in said test tube rack in accordance with a computer-controlled test program for controlling formation of fibrin clots in each of said test tubes, d) a heated bead-drop station on said support surface wherein substantially identical beads are simultaneously introduced into each test tube and wherein means are provided for timing, reporting and recording the time required for each bead to reach the bottom of said test tubes upon dissolving of said fibrin-clot therein, e) a robotic pick-and-place apparatus on said frame structure having pick-up and release means at the end of an arm thereof which arm comprising means for moving both vertically and horizontally, wherein said robotic apparatus comprising means for picking-up, said test tubes said test tube rack filled with empty test tubes from said loading station and for moving said test tube rack to said pipetting station and then to said heated bead-drop station and then back to said loading station in accordance with said computer controlled program, and f) a programmable computer for controlling said robotic apparatus, said pipetting apparatus, and the operations at said bead-drop station and for receiving and recording the results of said product testing.
Claim 2
Dependent Claim
313 words
An automated testing system for quality testing production samples of a blood-clot dissolving product comprising, a) a frame structure having a horizontally disposed support surface, b) a loading station on said support surface for receiving a test tube rack containing a plurality of test tubes, c) a pipetting station on said support surface characterized by an automatic computer-controlled pipetting apparatus and by reservoirs of buffer, media, plasma, fibrinogen, thrombin, and plasminogen solutions as well as production samples of the blood-clot dissolving product to be tested, all of which solutions are maintained at a certain cooling temperature of from 2°-8° C., which pipetting apparatus comprises means for providing vortex dispensing and mixing of said solutions and production samples in test tubes positioned in said test tube rack in accordance with a computer-controlled test program for controlling formation of fibrin clots in each of said test tubes, d) a heated bead-drop station on said support surface wherein substantially identical beads are simultaneously introduced into each test tube and wherein means are provided for timing, reporting and recording the time required for each bead to reach the bottom of said test tubes upon dissolving of said fibrin-clot therein, e) a robotic pick-and-place apparatus on said frame structure having pick-up and release means at the end of an arm thereof which arm comprising means for moving both vertically and horizontally, wherein said robotic apparatus comprising means for picking-up, said test tubes said test tube rack filled with empty test tubes from said loading station and for moving said test tube rack to said pipetting station and then to said heated bead-drop station and then back to said loading station in accordance with said computer controlled program, and f) a programmable computer for controlling said robotic apparatus, said pipetting apparatus, and the operations at said bead-drop station and for receiving and recording the results of said product testing.
Claim 3
Dependent Claim
47 words
The automated testing system of claim 1 wherein a pair of loading stations, a pair of robotic apparatus, and a pair of bead-drop stations are provided on said support surface and wherein a computer program is provided which results in twice as many sample tests being conducted.
Claim 4
Dependent Claim
56 words
The automated testing system of claim 2 wherein said pair of loading stations are both disposed at said pipetting station whereby said pair of robotic apparatus are programmed to move a pair of racks of test tubes only from said pipetting station to said pair of bead-drop stations and back to said pair of loading stations.
Claim 5
Dependent Claim
24 words
The automated testing system of claim 1 wherein said frame structure is mounted on rollers to facilitate movement of same from place to place.
Claim 6
Dependent Claim
59 words
The automated testing system of claim 1 wherein means are provided at said pipetting station for maintaining said reservoirs of solutions and said test tubes, after said samples and solutions have been dispensed and mixed therein, at a cooling temperature of from 2°-8° C. until said test tube rack is moved from said pipetting station to said bead-drop station.
Claim 7
Dependent Claim
32 words
The automated testing system of claim 1 wherein a water bath heated to a temperature of 37° C. (+/-1°) is provided at said bead-drop station for receiving said rack of test tubes.
Claim 8
Dependent Claim
44 words
The automated testing system of claim 1 wherein fiberoptic timer means are provided at said bead-drop station for each test tube, each fiberoptic timer means comprises being activated for measuring the length of time required for the fibrin-clot in that test tube to dissolve.
Claim 9
Dependent Claim
19 words
The automated testing system of claim 1 wherein the fibrin-clot dissolving product to be tested is the enzyme urokinase.
Claim 10
Dependent Claim
290 words
An automated testing system for quality testing production samples of urokinase, a clot dissolving enzyme, comprising, a) a test tube rack loading station, b) a test tube rack containing a plurality of test tubes, c) a fibrin-clot forming station characterized by reservoirs of fibrin-clotting solutions and production samples of urokinase to be tested, comprising means for maintaining temperature of said solutions and samples at said station at 2°-8° C., and by an automatic computer-controlled pippeting apparatus comprising means for providing dispensing and mixing of said solutions and production samples in said test tubes positioned in said rack, when said rack is disposed at said fibrin-clot forming station, in accordance with a computer-controlled test program, for controlling formation of fibrin-clots in each of said test tubes, d) a bead-drop station comprising means for introducing substantially similar opaque beads into each test tube, by a heated water bath for said rack of fibrin-clot-containing test tubes into which said beads were introduced to aid dissolution of said fibrin-clots as a result of warming thereof, and further comprising means for timing, reporting and recording the time required for each bead to pass through said fibrin-clot upon dissolution thereof, e) a robotic apparatus having pick-up and release means for picking up said test tube rack filled with empty test tubes from said loading station and for moving test tube rack to said fibrin-clot forming station and then to said bead-drop station and then back to said loading station for unloading of said rack in accordance with said computer-controlled program, and f) a pre-programmed computer for controlling said robotic apparatus, said pipetting apparatus, and the operations at said bead-drop station and for receiving and recording the results of said testing of said production samples of urokinase.
Claim 11
Dependent Claim
290 words
An automated testing system for quality testing production samples of urokinase, a clot dissolving enzyme, comprising, a) a test tube rack loading station, b) a test tube rack containing a plurality of test tubes, c) a fibrin-clot forming station characterized by reservoirs of fibrin-clotting solutions and production samples of urokinase to be tested, comprising means for maintaining temperature of said solutions and samples at said station at 2°-8° C., and by an automatic computer-controlled pippeting apparatus comprising means for providing dispensing and mixing of said solutions and production samples in said test tubes positioned in said rack, when said rack is disposed at said fibrin-clot forming station, in accordance with a computer-controlled test program, for controlling formation of fibrin-clots in each of said test tubes, d) a bead-drop station comprising means for introducing substantially similar opaque beads into each test tube, by a heated water bath for said rack of fibrin-clot-containing test tubes into which said beads were introduced to aid dissolution of said fibrin-clots as a result of warming thereof, and further comprising means for timing, reporting and recording the time required for each bead to pass through said fibrin-clot upon dissolution thereof, e) a robotic apparatus having pick-up and release means for picking up said test tube rack filled with empty test tubes from said loading station and for moving test tube rack to said fibrin-clot forming station and then to said bead-drop station and then back to said loading station for unloading of said rack in accordance with said computer-controlled program, and f) a pre-programmed computer for controlling said robotic apparatus, said pipetting apparatus, and the operations at said bead-drop station and for receiving and recording the results of said testing of said production samples of urokinase.
Claim 12
Dependent Claim
29 words
The automated testing system of claim 9 wherein vortex dispensing and mixing of said solutions and production samples in said test tubes is provided at said fibrin-clot forming station.
Claim 13
Dependent Claim
44 words
The automated testing system of claim 9 wherein fiberoptic timer means are provided at said bead-drop station for each test tube, each fiberoptic timer means comprises being activated for measuring the length of time required for the fibrin-clot in that test tube to dissolve.
Claim 14
Dependent Claim
35 words
The automated testing system of claim 9 wherein said test tube rack loading station, said fibrin-clot forming station, said bead-drop station, and said robotic apparatus are all mounted on a frame structure mounted on rollers.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-08 | 1997-05-10 | 1997-11-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-08 | 2001-05-10 | 2001-11-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-08 | 2005-05-10 | 2005-11-09 | $7700.00 | Window Closed / Lapsed |