US5260271A
Expired / Lapsed
US
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Nisin compositions for use as enhanced broad range bactericides
Assignee: Applied Microbiology Inc
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Filing: 1992-04-17
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Granted: 1993-11-09
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Expires: 2012-04-17
Abstract
Abstract Bacteriocin compositions comprising lanthionine containing bacteriocins and non-bactericidal agents. When the bacteriocin compositions are combined with a suitable carrier with each component present in sufficient quantities such that the composition is effective against Gram negative bacteria in addition to Gram positive bacteria, they become enhanced, rapid acting, broad range bactericides suitable for a variety of applications.
Inventors
- 👤 Peter Blackburn
- 👤 June Polak
- 👤 Sara-Ann Gusik
- 👤 Stephen D. Rubino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 10:01
CPC Classifications
A; A61; A61Q; A61Q11/00; A01; A01N; A01N37/00; A01N37/44; A01N37/46; A01N63/00; A01N63/50; A23; A23B; A23B11/00; A23B11/60; A23B11/65; A23B11/67; A23B2/00; A23B2/70; A23B2/725; A23B2/729; A23B2/7295; A23B2/767; A23B2/783; A23C; A23C9/00; A23C9/152; A23C9/158; A23C9/1585; A61K; A61K38/00; A61K38/16; A61K38/164; A61K8/00; A61K8/18; A61K8/30; A61K8/33; A61K8/37; A61K8/375; A61K8/40; A61K8/41; A61K8/416; A61K8/44; A61K8/64
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
78 words
A lanthionine-containing-bacteriocin bactericidal composition comprising a lanthionine-containing bacteriocin in combination with a chelating agent wherein the bacteriocin and the chelating agent are present in quantities such that the composition is effective against gram negative bacteria as measured by effectiveness against Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacteroides gingivalis and Actinobacillus actinomycetescomitans and has enhanced effectiveness against gram positive bacteria as measured by enhanced effectiveness against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Streptococcus agalactiae and Coryneform bacteria.
Claim 2
Dependent Claim
78 words
A lanthionine-containing-bacteriocin bactericidal composition comprising a lanthionine-containing bacteriocin in combination with a chelating agent wherein the bacteriocin and the chelating agent are present in quantities such that the composition is effective against gram negative bacteria as measured by effectiveness against Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacteroides gingivalis and Actinobacillus actinomycetescomitans and has enhanced effectiveness against gram positive bacteria as measured by enhanced effectiveness against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Streptococcus agalactiae and Coryneform bacteria.
Claim 3
Dependent Claim
100 words
A lanthionine-containing-bacteriocin bactericidal composition comprising a lanthionine-containing bacteriocin, in combination with a chelating agent and a surfactant selected from the group consisting of glycerides, fatty acids, emulsifiers, quaternary compounds, amphoteric surfactants and nonionic surfactants wherein the bacteriocin, the chelating agent and the surfactant are present in quantities such that the composition is effective against gram negative bacteria as measured by effectiveness against Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacteroides gingivalis and Actinobacillus actinomycetescomitans and has enhanced effectiveness against gram positive bacteria as measured by enhanced effectiveness against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Streptococcus agalactiae and Coryneform bacteria.
Claim 4
Dependent Claim
100 words
A lanthionine-containing-bacteriocin bactericidal composition comprising a lanthionine-containing bacteriocin, in combination with a chelating agent and a surfactant selected from the group consisting of glycerides, fatty acids, emulsifiers, quaternary compounds, amphoteric surfactants and nonionic surfactants wherein the bacteriocin, the chelating agent and the surfactant are present in quantities such that the composition is effective against gram negative bacteria as measured by effectiveness against Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacteroides gingivalis and Actinobacillus actinomycetescomitans and has enhanced effectiveness against gram positive bacteria as measured by enhanced effectiveness against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Streptococcus agalactiae and Coryneform bacteria.
Claim 5
Dependent Claim
31 words
The bactericidal composition as defined in claim 1 or 2 wherein the lanthionine containing bacteriocin is selected from the group consisting of nisin, subtilin, epidermin, cinnamycin, duramycin, ancovenin and Pep 5.
Claim 6
Dependent Claim
30 words
The bactericidal composition as defined in claim 1 or 2 wherein the chelating agent is selected from the group consisting of alkyldiamine tetracetates, CaEDTA, Na 2 CaEDTA, EGTA and citrate.
Claim 7
Dependent Claim
19 words
The bactericidal composition as defined in claim 4 wherein the alkyldiamine tetraacetate is EDTA and the bacteriocin is nisin.
Claim 8
Dependent Claim
13 words
The bactericidal composition as defined in claim 1 also containing a food preservative.
Claim 9
Dependent Claim
80 words
A nisin-containing bactericidal composition comprising nisin in combination with a chelating agent in a suitable carrier wherein the nisin and the chelating agent are present in quantities such that the composition is effective against gram negative bacteria as measured by effectiveness against Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacteroides gingivalis and Actinobacillus actinomycetescomitans and has enhanced effectiveness against gram positive bacteria as measured by enhanced effectiveness against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Streptococcus agalactiae and Coryneform bacteria.
Claim 10
Dependent Claim
80 words
A nisin-containing bactericidal composition comprising nisin in combination with a chelating agent in a suitable carrier wherein the nisin and the chelating agent are present in quantities such that the composition is effective against gram negative bacteria as measured by effectiveness against Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacteroides gingivalis and Actinobacillus actinomycetescomitans and has enhanced effectiveness against gram positive bacteria as measured by enhanced effectiveness against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Streptococcus agalactiae and Coryneform bacteria.
Claim 11
Dependent Claim
28 words
The bactericidal composition of claim 7 which also comprises a surfactant selected from the group consisting of glycerides, fatty acids, emulsifiers, quaternary compounds, amphoteric surfactants and nonionic surfactants.
Claim 12
Dependent Claim
36 words
The bactericidal composition as in claim 5 or 7 wherein the concentration of nisin is between about 0.1 μg/ml and 300.0 μg/ml and the concentration of chelating agent is between about 0.1 mM and 20 mM.
Claim 13
Dependent Claim
20 words
The bactericidal composition as in claim 2 or 8 wherein the concentration of surfactant is between about 0.01% and 1.0%.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-08 | 1997-05-10 | 1997-11-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-08 | 2001-05-10 | 2001-11-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-08 | 2005-05-10 | 2005-11-09 | $7700.00 | Window Closed / Lapsed |