Patent US5260192A successfully refreshed from Google Patents.
US5260192A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for screening cells or formed bodies with populations expressing selected characteristics utilizing at least one sensing parameter
Assignee: Beckman Coulter Inc
•
Filing: 1992-08-12
•
Granted: 1993-11-09
•
Expires: 2012-08-12
Abstract
Abstract A method and apparatus for automatically and rapidly, retrieving counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. A multipart blood cell analysis is obtained with a single sensing parameter by depleting at least one WBC subset population. The percentage of a desired WBC population subset or the overlapping of WBC subset populations also can be obtained by subtracting one or more obscuring WBC subset populations. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
Inventors
- 👤 Thomas Russell
- 👤 Constance M. Hajek
- 👤 Carlos M. Rodriguez
- 👤 Wallace H. Coulter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 20:44
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N1/00; G01N1/28; G01N15/1031; G01N15/12; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N33/53; G01N33/5302; G01N33/543; G01N33/54313; G01N33/54326; G01N33/569; G01N33/56966; G01N33/56972; G01N15/14; G01N15/149; G01N15/01; G01N2015/016; G01N2015/1019; G01N2015/1024; G01N2015/1028; G01N2015/1486; G01N35/00; G01N35/0098; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/11; Y10T436/117497
Claims Analysis
Total Claims: 58
Claim 1
Independent Claim
181 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations and/or subset populations therein, comprising: analyzing at least a first portion of said whole blood sample to determine at least one white blood cell populations or subset population characteristic of said whole blood sample by Coulter sensing or light scattering techniques; substantially depleting at least one white blood cell population or subset population thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population; analyzing said second portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said second portion by Coulter sensing or light scattering techniques; and comparing said two analyzed characteristics to determine the contribution of at least one white blood cell population or subset population of said whole blood sample.
Claim 2
Independent Claim
181 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations and/or subset populations therein, comprising: analyzing at least a first portion of said whole blood sample to determine at least one white blood cell populations or subset population characteristic of said whole blood sample by Coulter sensing or light scattering techniques; substantially depleting at least one white blood cell population or subset population thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population; analyzing said second portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said second portion by Coulter sensing or light scattering techniques; and comparing said two analyzed characteristics to determine the contribution of at least one white blood cell population or subset population of said whole blood sample.
Claim 3
Dependent Claim
98 words
The method as defined in claim 1 including subtracting said second portion white blood cell population or subset population by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population and mixing said magnetic microspheres with said sample to bind to said white blood cell population or subset population, attracting said magnetic microspheres with a magnetic field and removing said white blood cell population or subset population by removing at least a portion of the remainder of said sample while said magnetic microspheres are bound in said magnetic field.
Claim 4
Dependent Claim
31 words
The method as defined in claim 1 including subtracting at least the neutrophil population from said second portion white blood cell population or subset population prior to analyzing said second portion.
Claim 5
Dependent Claim
83 words
The method as defined in claim 1 including subtracting at least two white blood cell populations or subset populations from at least a third portion of said whole blood sample; analyzing said third portion of said whole blood sample to determine at least one white blood cell population or subset population characteristics of said whole blood sample; and comparing said three analyzed characteristics to determine the percentage of at least two white blood cell populations or subset populations of said whole blood sample.
Claim 6
Dependent Claim
91 words
The method as defined in claim 4 including subtracting said third portion white blood cell populations or subset populations by providing magnetic microspheres having at least one monoclonal antibody bonded thereto specific to said white blood cell populations or subset populations and mixing said magnetic microspheres with said sample to bind to said white blood cell populations or subset populations and removing said white blood cell populations or subset populations by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 7
Dependent Claim
30 words
The method as defined in claim 4 including subtracting at least the neutrophil and eosinophiI populations from said third portion white blood cell population prior to analyzing said third portion.
Claim 8
Dependent Claim
30 words
The method as defined in claim 4 including subtracting at least the lymphocyte and neutrophil populations from said third portion white blood cell population prior to analyzing said third portion.
Claim 9
Dependent Claim
85 words
The method as defined in claim 4 including subtracting at least two white blood cell populations or subset populations from at least a fourth portion of said whole blood sample; analyzing said fourth portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said whole blood sample; and comparing at least said four analyzed characteristics to determine the percentage of at least three white blood cell populations or subset populations of said whole blood sample.
Claim 10
Dependent Claim
52 words
The method as defined in claim 8 including comparing at least four analyzed white blood cell population characteristics to calculate or determine the percentage of at least the eosinophil's, monocyte's, lymphocyte's, basophil's and neutrophiI's white blood cell populations of said whole blood sample to perform a five part white blood cell differential.
Claim 11
Dependent Claim
48 words
The method as defined in claim 1 wherein said whole blood sample includes a red blood cell population and removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations of interest.
Claim 12
Dependent Claim
17 words
The method as defined in claim 1 including electronically analyzing said portions utilizing a single electronic parameter.
Claim 13
Dependent Claim
17 words
The method as defined in claim 1 including optically analyzing said portions utilizing a single light parameter.
Claim 14
Dependent Claim
31 words
The method as defined in claim 1 including comparing said two analyzed characteristics to determine the percentage of at least two subset populations of a least one white blood cell population.
Claim 15
Independent Claim
189 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations and/or subset populations therein, comprising: means for analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said whole blood sample by Coulter sensing or light scattering techniques; means for substantially depleting at least one white blood cell population or subset population thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population; means for analyzing said second portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said second portion by Coulter sensing or light scattering techniques; and means for comparing said two analyzed characteristics to determine the contribution of at least one white blood cell population or subset population of said whole blood sample.
Claim 16
Independent Claim
189 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations and/or subset populations therein, comprising: means for analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said whole blood sample by Coulter sensing or light scattering techniques; means for substantially depleting at least one white blood cell population or subset population thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population; means for analyzing said second portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said second portion by Coulter sensing or light scattering techniques; and means for comparing said two analyzed characteristics to determine the contribution of at least one white blood cell population or subset population of said whole blood sample.
Claim 17
Dependent Claim
109 words
The apparatus as defined in claim 14 wherein said means for subtracting said second portion white blood cell population or subset population include means for providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population and means for mixing said magnetic microspheres with said sample to bind to said white blood cell population or subset population, means for attracting said magnetic microspheres with a magnetic field and means for removing said white blood cell population or subset population by removing at least a portion of the remainder of said sample while said magnetic microspheres are bound in said magnetic field.
Claim 18
Dependent Claim
33 words
The apparatus as defined in claim 14 including means for subtracting at least the neutrophil population from said second portion white blood cell population or subset population prior to analyzing said second portion.
Claim 19
Dependent Claim
89 words
The apparatus as defined in claim 14 including means for subtracting at least two white blood cell populations or subset populations from at least a third portion of said whole blood sample; means for analyzing said third portion of said whole blood sample to determine at least one white blood cell population or subset population characteristics of said whole blood sample; and means for comparing said three analyzed characteristics to determine the percentage of at least two white blood cell populations or subset populations of said whole blood sample.
Claim 20
Dependent Claim
100 words
The apparatus as defined in claim 17 wherein said means for subtracting said third portion white blood cell populations or subset populations include means for providing magnetic microspheres having at least one monoclonal antibody bonded thereto specific to said white blood cell populations or subset populations and means for mixing said magnetic microspheres with said sample to bind to said white blood cell populations or subset populations and means for removing said white blood cell populations or subset populations by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 21
Dependent Claim
32 words
The apparatus as defined in claim 17 including means for subtracting at least the neutrophil and eosinophii populations from said third portion white blood cell population prior to analyzing said third portion.
Claim 22
Dependent Claim
32 words
The apparatus as defined in claim 17 including means for subtracting at least the lymphocyte and neutrophil populations from said third portion white blood cell population prior to analyzing said third portion.
Claim 23
Dependent Claim
91 words
The apparatus as defined in claim 17 including means for subtracting at least two white blood cell populations or subset populations from at least a fourth portion of said whole blood sample; means for analyzing said fourth portion of said whole blood sample to determine at least one white blood cell population or subset population characteristic of said whole blood sample; and means for comparing at least said four analyzed characteristics to determine the percentage of at least three white blood cell populations or subset populations of said whole blood sample.
Claim 24
Dependent Claim
54 words
The apparatus as defined in claim 21 including means for comparing at least four analyzed white blood cell population characteristics to calculate or determine the percentage of at least the eosinophil's, monocyte's, lymphocyte's, basophil's and neutrophil's white blood cell populations of said whole blood sample to perform a five part white blood cell differential.
Claim 25
Dependent Claim
51 words
The apparatus as defined in claim 14 wherein said whole blood sample includes a red blood cell population and including means for removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations of interest.
Claim 26
Dependent Claim
19 words
The apparatus as defined in claim 14 including means for electronically analyzing said portions utilizing a single electronic parameter.
Claim 27
Dependent Claim
19 words
The apparatus as defined in claim 14 including means for optically analyzing said portions utilizing a single light parameter.
Claim 28
Dependent Claim
33 words
The apparatus as defined in claim 14 including means for comparing said two analyzed characteristics to determine the percentage of at least two subset populations of a least one white blood cell population.
Claim 29
Independent Claim
231 words
A method of exchanging and obtaining at least one white blood cell population or white blood cell population subset analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population or white blood cell population subset characteristic of said whole blood sample by Coulter sensing or light scattering techniques; subtracting at least one white blood cell population or white blood cell population subset from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population which white blood cell population or white blood cell population subset would obscure the analysis of the desired white blood cell population or white blood cell population subset; analyzing said second portion of said whole blood sample to determine at least one desired white blood cell population or white blood cell population subset characteristics of interest of said second portion by Coulter sensing or light scattering techniques; and comparing said two analyzed characteristics to determine the contribution of said at least one desired white blood cell population or white blood cell population subset of interest of said whole blood sample.
Claim 30
Independent Claim
231 words
A method of exchanging and obtaining at least one white blood cell population or white blood cell population subset analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population or white blood cell population subset characteristic of said whole blood sample by Coulter sensing or light scattering techniques; subtracting at least one white blood cell population or white blood cell population subset from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population which white blood cell population or white blood cell population subset would obscure the analysis of the desired white blood cell population or white blood cell population subset; analyzing said second portion of said whole blood sample to determine at least one desired white blood cell population or white blood cell population subset characteristics of interest of said second portion by Coulter sensing or light scattering techniques; and comparing said two analyzed characteristics to determine the contribution of said at least one desired white blood cell population or white blood cell population subset of interest of said whole blood sample.
Claim 31
Dependent Claim
101 words
The method as defined in claim 27 including subtracting said first portion white blood cell population or white blood cell population subset by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or white blood cell population subset and mixing said magnetic microspheres with said sample to bind to said white blood cell population or white blood cell population subset and removing said white blood cell population or white blood cell population subset by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 32
Dependent Claim
39 words
The method as defined in claim 27 including subtracting at least the lymphocyte population from said first portion white blood cell population prior to analyzing said first portion to obtain a basophil percentage population of said whole blood sample.
Claim 33
Dependent Claim
52 words
The method as defined in claim 27 including subtracting at least one white blood cell subset population of interest from said first portion of said whole blood sample prior to analyzing said first portion to obtain a percentage population of said white blood cell subset of interest of said whole blood sample.
Claim 34
Dependent Claim
51 words
The method as defined in claim 27 wherein said whole blood sample includes a red blood cell population and removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations or subset populations of interest.
Claim 35
Dependent Claim
17 words
The method as defined in claim 27 including electronically analyzing said portions utilizing a single electronic parameter.
Claim 36
Dependent Claim
17 words
The method as defined in claim 27 including optically analyzing said portions utilizing a single light parameter.
Claim 37
Dependent Claim
17 words
The method as defined in claim 27 including analyzing said portions utilizing at least two sensing parameters.
Claim 38
Independent Claim
239 words
An apparatus for enhancing and obtaining at least one white blood cell population or white blood cell population subset analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population or white blood cell population subset characteristic of said whole blood sample by Coulter sensing or light scattering techniques; means of subtracting at least one white blood cell population or white blood cell population subset from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population which white blood cell population or white blood cell population subset would obscure the analysis of the desired white blood cell population or white blood cell population subset; means for analyzing said second portion of said whole blood sample to determine at least one desired white blood cell population or white blood cell population subset characteristic of interest of said second portion by Coulter sensing or light scattering techniques; and means for comparing said two analyzed characteristics to determine the contribution of said at least one desired white blood cell population or white blood cell population subset of interest of said whole blood sample.
Claim 39
Independent Claim
239 words
An apparatus for enhancing and obtaining at least one white blood cell population or white blood cell population subset analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population or white blood cell population subset characteristic of said whole blood sample by Coulter sensing or light scattering techniques; means of subtracting at least one white blood cell population or white blood cell population subset from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or subset population to said white blood cell population or subset population which white blood cell population or white blood cell population subset would obscure the analysis of the desired white blood cell population or white blood cell population subset; means for analyzing said second portion of said whole blood sample to determine at least one desired white blood cell population or white blood cell population subset characteristic of interest of said second portion by Coulter sensing or light scattering techniques; and means for comparing said two analyzed characteristics to determine the contribution of said at least one desired white blood cell population or white blood cell population subset of interest of said whole blood sample.
Claim 40
Dependent Claim
107 words
The apparatus as defined in claim 35 including means for subtracting said first portion white blood cell population or white blood cell population subset including providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population or white blood cell population subset and means for mixing said magnetic microspheres with said sample to bind to said white blood cell population anchor white blood cell population subset and means for removing said white blood cell population or white blood cell population subset by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 41
Dependent Claim
41 words
The apparatus as defined in claim 35 including means for subtracting at least the lymphocyte population from said first portion white blood cell population prior to analyzing said first portion to obtain a basophil percentage population of said whole blood sample.
Claim 42
Dependent Claim
54 words
The apparatus as defined in claim 35 including means for subtracting at least one white blood cell subset population of interest from said first portion of said whole blood sample prior to analyzing said first portion to obtain a percentage population of said white blood cell subset of interest of said whole blood sample.
Claim 43
Dependent Claim
54 words
The apparatus as defined in claim 35 wherein said whole blood sample includes a red blood cell population and including means for removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations or subset populations of interest.
Claim 44
Dependent Claim
19 words
The apparatus as defined in claim 35 including means for electronically analyzing said portions utilizing a single electronic parameter.
Claim 45
Dependent Claim
19 words
The apparatus as defined in claim 35 including means for optically analyzing said portions utilizing a single light parameter.
Claim 46
Dependent Claim
19 words
The apparatus as defined in claim 35 including means for analyzing said portions utilizing at least two sensing parameters.
Claim 47
Independent Claim
267 words
A method of obtaining the contribution of overlapping populations of at least two white blood cell a subset populations from at least a portion of a whole blood sample having at least the two subset populations; comprising: analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population characteristic of said whole blood sample by Coulter sensing or light scattering techniques; substantially depleting at least a first one of said white blood cell subset populations thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said first white blood cell subset population to said first white blood cell subset population; analyzing said second depleted portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said first subset population by Coulter sensing or light scattering techniques; substantially depleting at least a second one of said white blood cell subset populations thereof from at least a third portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said second white blood cell subset population to said second white blood cell subset population; analyzing said third depleted portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said second subset population by Coulter sensing or light scattering techniques; and comparing said three analyzed characteristics to determine the overlapping of antigens of said two third blood cell subset populations of said whole blood sample.
Claim 48
Independent Claim
267 words
A method of obtaining the contribution of overlapping populations of at least two white blood cell a subset populations from at least a portion of a whole blood sample having at least the two subset populations; comprising: analyzing at least a first portion of said whole blood sample to determine at least one white blood cell population characteristic of said whole blood sample by Coulter sensing or light scattering techniques; substantially depleting at least a first one of said white blood cell subset populations thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said first white blood cell subset population to said first white blood cell subset population; analyzing said second depleted portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said first subset population by Coulter sensing or light scattering techniques; substantially depleting at least a second one of said white blood cell subset populations thereof from at least a third portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said second white blood cell subset population to said second white blood cell subset population; analyzing said third depleted portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said second subset population by Coulter sensing or light scattering techniques; and comparing said three analyzed characteristics to determine the overlapping of antigens of said two third blood cell subset populations of said whole blood sample.
Claim 49
Dependent Claim
81 words
The method as defined in claim 43 including substantially depleting at least both said first and second white blood cell subset populations from at least a fourth portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said first and second white blood cell subset populations; and comparing said four analyzed characteristics to determine the percentage of overlapping antigens of said two white blood cell subset populations of said whole blood sample.
Claim 50
Dependent Claim
17 words
The method as defined in claim 43 including electronically analyzing said portions utilizing a single electronic parameter.
Claim 51
Dependent Claim
17 words
The method as defined in claim 43 including optically analyzing said portions utilizing a single light parameter.
Claim 52
Dependent Claim
17 words
The method as defined in claim 43 including analyzing said portions utilizing at least two sensing parameters.
Claim 53
Independent Claim
278 words
An apparatus for obtaining the contribution of overlapping populations of at least two white blood cell subset populations from at least a portion of a whole blood sample having at least the two subset populations; comprising: means for analyzing at least a first portion of said whole blood sample to determine at least one white blood cell populations characteristic of said whole blood sample by Coulter sensing or light scattering techniques; means for substantially depleting at least a first one of said white blood cell subset populations thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said first white blood cell subset population to said first white blood cell subset population; means for analyzing said second depleted portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said first subset population by Coulter sensing or light scattering techniques; means for substantially depleting at least a second one of said white blood cell subset populations thereof from at least a third portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said second white blood cell subset population to said second white blood cell subset population; means for analyzing said third depleted portion of said whole blood sample to determine at least one characteristics of said white blood cell population or said second subset population by Coulter sensing or light scattering techniques; and means for comparing said three analyzed characteristics to determine the overlapping of antigens of said two white blood cell subset populations of said whole blood sample.
Claim 54
Independent Claim
278 words
An apparatus for obtaining the contribution of overlapping populations of at least two white blood cell subset populations from at least a portion of a whole blood sample having at least the two subset populations; comprising: means for analyzing at least a first portion of said whole blood sample to determine at least one white blood cell populations characteristic of said whole blood sample by Coulter sensing or light scattering techniques; means for substantially depleting at least a first one of said white blood cell subset populations thereof from at least a second portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said first white blood cell subset population to said first white blood cell subset population; means for analyzing said second depleted portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said first subset population by Coulter sensing or light scattering techniques; means for substantially depleting at least a second one of said white blood cell subset populations thereof from at least a third portion of said whole blood sample by binding microspheres having a monoclonal antibody bonded thereto specific to said second white blood cell subset population to said second white blood cell subset population; means for analyzing said third depleted portion of said whole blood sample to determine at least one characteristics of said white blood cell population or said second subset population by Coulter sensing or light scattering techniques; and means for comparing said three analyzed characteristics to determine the overlapping of antigens of said two white blood cell subset populations of said whole blood sample.
Claim 55
Dependent Claim
85 words
The apparatus as defined in claim 48 including means for substantially depleting at least both said first and second white blood cell subset populations from at least a fourth portion of said whole blood sample to determine at least one characteristic of said white blood cell population or said first and second white blood cell subset populations; and means for comparing said four analyzed characteristics to determine the percentage of overlapping antigens of said two white blood cell subset populations of said whole blood sample.
Claim 56
Dependent Claim
19 words
The apparatus as defined in claim 48 including means for electronically analyzing said portions utilizing a single electronic parameter.
Claim 57
Dependent Claim
19 words
The apparatus as defined in claim 48 including means for optically analyzing said portions utilizing a single light parameter.
Claim 58
Dependent Claim
19 words
The apparatus as defined in claim 48 including means for analyzing said portions utilizing at least two sensing parameters.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-08 | 1997-05-10 | 1997-11-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-08 | 2001-05-10 | 2001-11-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-08 | 2005-05-10 | 2005-11-09 | $7700.00 | Window Closed / Lapsed |