Patent US5232829A successfully refreshed from Google Patents.
US5232829A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
Assignee: Roche Molecular Systems Inc
•
Filing: 1989-09-29
•
Granted: 1993-08-03
•
Expires: 2009-09-29
Abstract
Abstract A format for hybridization capture of PCR amplified DNA on a solid support is provided. After labelling the amplified target DNA with biotin during amplification, the labelled DNA is specifically captured by base-pair hybridization to an amplicon-specific oligonucleotide capture probe which is bound to a solid support and the labeled DNA is detected with a biotin dependent chromogenic detection assay. In a specific format selected primers and probes are disclosed which enable the detection of Chlamydia trachomatis.
Inventors
- 👤 Mathew Longiaru
- 👤 Sheryl B. Silver
- 👤 Michael A. Sulzinski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 19:37
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6832; C12Q1/6876; C12Q1/6888; C12Q1/689
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
70 words
A diagnostic kit for the detection of a target nucleic acid sequence of Chlamydia trachomatis, said kit comprising PCR primers wherein said primers are oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCTGACTTGTTGTTACAGGAATCCC 3' and 5' GTGTTCTTATTGTTCTGGGGAAGAGG 3' and a microtiter plate having a plurality of wells and having bound thereto oligonucleotide capture probe having a nucleic acid sequence substantially complementary to said target sequence.
Claim 2
Dependent Claim
70 words
A diagnostic kit for the detection of a target nucleic acid sequence of Chlamydia trachomatis, said kit comprising PCR primers wherein said primers are oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCTGACTTGTTGTTACAGGAATCCC 3' and 5' GTGTTCTTATTGTTCTGGGGAAGAGG 3' and a microtiter plate having a plurality of wells and having bound thereto oligonucleotide capture probe having a nucleic acid sequence substantially complementary to said target sequence.
Claim 3
Dependent Claim
25 words
The diagnostic kit of claim 1 which additionally comprises reagents for conducting the PCR amplification and hybridization of said capture probe and said target sequence.
Claim 4
Dependent Claim
13 words
The diagnostic kit of claim 2 which additionally comprises Tao polymerase and dNTP's.
Claim 5
Dependent Claim
17 words
The diagnostic kit of claim 3 wherein one or more of said dNTP's comprise a labelling agent.
Claim 6
Dependent Claim
25 words
The diagnostic kit of claim 1 wherein at least one of said PCR primers is biotinylated through a linker arm modification at the 5' terminus.
Claim 7
Dependent Claim
17 words
The diagnostic kit of claim 1 wherein said microtiter plate comprises polystyrene having enhanced protein binding capacity.
Claim 8
Dependent Claim
22 words
The diagnostic kit of claim 1 wherein said probe is a oligonucleotide that will bind to the following sequence: 5' GGCTTGCAGAGTTCTATAGTGCTATG 3'.
Claim 9
Dependent Claim
38 words
The diagnostic kit of claim 1 which is suitable for detection of Chlamydia trachomatis and wherein said primers are oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCGCACGTTCTCTCAAGCAGGAC 3' and 5' GTCTGACGGTTCTTAAGCTGGGAG 3'.
Claim 10
Dependent Claim
22 words
The diagnostic kit of claim 8 wherein said probe is a oligonucleotide that will bind to the following sequence: 5' TTCCGGAGCGAGTTACGAAGACA 3'.
Claim 11
Dependent Claim
36 words
Primers for use in the PCR amplification of Chlamydia trachomatis nucleic acid target sequence, said primers being oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCTGACTTGTTGTTACAGGAATCCC 3' and 5' GTGTTCTTATTGTTCTGGGGAAGAGG 3'.
Claim 12
Dependent Claim
36 words
Primers for use in the PCR amplification of Chlamydia trachomatis nucleic acid target sequence, said primers being oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCTGACTTGTTGTTACAGGAATCCC 3' and 5' GTGTTCTTATTGTTCTGGGGAAGAGG 3'.
Claim 13
Dependent Claim
29 words
Probes useful in hybridization assays of DNA sequences amplified with the primers of claim 10, said probes being oligonucleotides that will bind to the following sequence: 5' GGCTTGCAGAGTTCTATAGTGCTATG 3'.
Claim 14
Dependent Claim
36 words
Primers for use in the PCR amplification of Chlamydia trachomatis nucleic acid target sequence, said primers being oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCGCACGTTCTCTCAAGCAGGAC 3' and 5' GTCTGACGGTTCTTAAGCTGGGAG 3'.
Claim 15
Dependent Claim
36 words
Primers for use in the PCR amplification of Chlamydia trachomatis nucleic acid target sequence, said primers being oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCGCACGTTCTCTCAAGCAGGAC 3' and 5' GTCTGACGGTTCTTAAGCTGGGAG 3'.
Claim 16
Dependent Claim
30 words
Probes useful in hybridization assays of DNA sequences amplified with the primers of claim 12, said probe being an oligonucleotide that will bind to the following sequence: 5' TTCCGGAGCGAGTTACGAAGACA 3'.
Claim 17
Independent Claim
40 words
A method of detecting Chlamydia trachomatis by PCR amplification and hybridization assay comprising utilizing in said PCR amplification primers which are oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCTGACTTGTTGTTACAGGAATCCC 3' and 5' GTGTTCTTATTGTTCTGGGGAAGAGG 3'.
Claim 18
Independent Claim
40 words
A method of detecting Chlamydia trachomatis by PCR amplification and hybridization assay comprising utilizing in said PCR amplification primers which are oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCTGACTTGTTGTTACAGGAATCCC 3' and 5' GTGTTCTTATTGTTCTGGGGAAGAGG 3'.
Claim 19
Dependent Claim
32 words
The method of claim 44 wherein said hybridization assay further utilizes a capture probe and wherein said capture probe is an oligonucleotide that will bind to the following sequence: 5' GGCTTGCAGAGTTCTATAGTGCTATG 3'.
Claim 20
Independent Claim
40 words
A method of detecting Chlamydia trachomatic by PCR amplification and hybridization assay comprising utilizing in said PCR amplification primers which are oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCGCACGTTCTCTCAAGCAGGAC 3' and 5' GTCTGACGGTTCTTAAGCTGGGAG 3'.
Claim 21
Independent Claim
40 words
A method of detecting Chlamydia trachomatic by PCR amplification and hybridization assay comprising utilizing in said PCR amplification primers which are oligonucleotides that will bind to or cause elongation through the following sequences: 5' CCGCACGTTCTCTCAAGCAGGAC 3' and 5' GTCTGACGGTTCTTAAGCTGGGAG 3'.
References Cited (Backward Citations)
US4154795A
Cited
US4228237A
Cited
US4358535A
Cited
US4358535B1
Cited
WO1983001459A1
Cited
US4486539A
Cited
US4725388A
Cited
GB2139349A
Cited
US4731325A
Cited
WO1986005815A1
Cited
US4683202A
Cited
US4683202B1
Cited
US4751177A
Cited
US4797355A
Cited
US4822731A
Cited
US4683195A
Cited
US4683195B1
Cited
GB2187283A
Cited
EP0237362A1
Cited
WO1987006621A1
Cited
GB2202328A
Cited
EP0302715A1
Cited
EP0336412A2
Cited
WO1989011548A1
Cited
EP0439222A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-08-02 | 1997-02-01 | 1997-08-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-08-02 | 2001-02-01 | 2001-08-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-08-02 | 2005-02-01 | 2005-08-03 | $7700.00 | Window Closed / Lapsed |