Patent US5231005A successfully refreshed from Google Patents.
US5231005A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for screening cells or formed bodies with populations expressing selected characteristics
Assignee: Beckman Coulter Inc
•
Filing: 1992-03-10
•
Granted: 1993-07-27
•
Expires: 2012-03-10
Abstract
Abstract A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
Inventors
- 👤 Thomas Russell
- 👤 Kenneth H. Kortright
- 👤 Wallace H. Coulter
- 👤 Carlos M. Rodriguez
- 👤 Ronald Paul
- 👤 Constance M. Hajek
- 👤 James C. Hudson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 21:14
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/1031; G01N15/12; G01N33/00; G01N33/48; G01N33/50; G01N33/5002; G01N33/5005; G01N33/5094; G01N33/53; G01N33/5302; G01N33/543; G01N33/54313; G01N33/54326; G01N33/569; G01N33/56966; G01N33/56972; G01N15/14; G01N15/01; G01N2015/016; G01N2015/1019; G01N2015/1024; G01N2015/1028; G01N35/00; G01N35/0098; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/11; Y10T436/117497; Y10T436/24
Claims Analysis
Total Claims: 174
Claim 1
Independent Claim
144 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: modifying the volume and/or opacity parameters of at least one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 2
Independent Claim
144 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: modifying the volume and/or opacity parameters of at least one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 3
Dependent Claim
32 words
The method as defined in claim 1 including subtracting at least one white blood cell population from said white blood cell populations prior to analyzing said modified white blood cell population subset.
Claim 4
Dependent Claim
75 words
The method as defined in claim 2 including subtracting said white blood cell population by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population and mixing said magnetic microspheres with said sample to bind to said white blood cell population and removing said white blood cell population by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 5
Dependent Claim
32 words
The method as defined in claim 1 including subtracting at least the neutrophil and eosinophil populations from said white blood cell population prior to analyzing said modified white blood cell population subset.
Claim 6
Dependent Claim
75 words
The method as defined in claim 4 including subtracting said neutrophil and eosinophil populations by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said neutrophil and eosinophil populations and mixing said magnetic microspheres with said sample to bind to said neutrophil and eosinophil populations and removing said neutrophil and eosinophil populations by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 7
Dependent Claim
63 words
The method as defined in claim 1 including modifying the CD4 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD4 white blood cell population subset and mixing said microspheres with said sample to bind to said CD4 subset population to shift at least one electronic characteristic of said CD4 subset population.
Claim 8
Dependent Claim
63 words
The method as defined in claim 1 including modifying the CD8 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD8 white blood cell population subset and mixing said microspheres with said sample to bind to said CD8 subset population to shift at least one electronic characteristic of said CD8 subset population.
Claim 9
Dependent Claim
63 words
The method as defined in claim 1 including modifying the CD2 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD2 white blood cell population subset and mixing said microspheres with said sample to bind to said CD2 subset population to shift at least one electronic characteristic of said CD2 subset population.
Claim 10
Dependent Claim
63 words
The method as defined in claim 1 including modifying the CD20 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD20 white blood cell population subset and mixing said microspheres with said sample to bind to said CD20 subset population to shift at least one electronic characteristic of said CD20 subset population.
Claim 11
Dependent Claim
48 words
The method as defined in claim 1 wherein said whole blood sample includes a red blood cell population and removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations of interest.
Claim 12
Dependent Claim
60 words
The method as defined in claim 10 wherein removal of said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population; and removing said microspheres with said bound red blood cells from said whole blood sample.
Claim 13
Dependent Claim
34 words
The method as defined in claim 11 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 14
Dependent Claim
29 words
The method as defined in claim 10 wherein removing said red blood cell population includes providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 15
Dependent Claim
98 words
The method as defined in claim 1 including modifying the volume and/or opacity parameters of at least a second white blood cell subset of said white blood cell populations of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and electronically analyzing at least one of said modified white blood cell subset sand said selected white blood cell population of interest to determine at least one characteristic of said selected white blood cell population.
Claim 16
Dependent Claim
18 words
The method as defined in claim 14 including electronically analyzing both of said modified white blood cell subsets.
Claim 17
Dependent Claim
77 words
The method as defined in claim 14 including modifying said two white blood cell subsets by providing microspheres of a first size having a monoclonal antibody bonded thereto specific to said first white blood cell subset and providing microspheres of a second size, different from said first size, having a monoclonal antibody bonded thereto specific to said second white blood cell subset, and mixing said microspheres with said sample to bind to said white blood cell populations.
Claim 18
Dependent Claim
95 words
The method as defined in claim 1 including modifying said white blood cell population subset by providing a first set of microspheres having a monoclonal antibody bonded thereto specific to said white blood cell subset and mixing said microspheres with said sample to bind to said white blood cell populations and then providing a second set of microspheres having a monoclonal antibody bonded thereto specific to said monoclonal antibody bonded onto said first set of microspheres and mixing said second set of microspheres with said sample and said first set of microspheres to bind thereto.
Claim 19
Dependent Claim
24 words
The method as defined in claim 10 including modifying said white blood cell population subset and removing said red blood cell population substantially simultaneously.
Claim 20
Dependent Claim
23 words
The method as defined in claim 10 including modifying said white blood cell population subset and removing said red blood cell population sequentially.
Claim 21
Independent Claim
148 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: means for modifying the volume and/or opacity parameters of at least one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 22
Independent Claim
148 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: means for modifying the volume and/or opacity parameters of at least one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 23
Dependent Claim
34 words
The apparatus as defined in claim 20 including means for subtracting at least one white blood cell population from said white blood cell populations prior to analyzing said modified white blood cell population subset.
Claim 24
Dependent Claim
81 words
The apparatus as defined in claim 21 including means for subtracting said white blood cell population by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population and means for mixing said magnetic microspheres with said sample to bind to said white blood cell population and means for removing said white blood cell population by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 25
Dependent Claim
34 words
The apparatus as defined in claim 20 including means for subtracting at least the neutrophil and eosinophil populations from said white blood cell population prior to analyzing said modified white blood cell population subset.
Claim 26
Dependent Claim
81 words
The apparatus as defined in claim 23 including means for subtracting said neutrophil and eosinophil populations by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said neutrophil and eosinophil populations and means for mixing said magnetic microspheres with said sample to bind to said neutrophil and eosinophil populations and means for removing said neutrophil and eosinophil populations by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 27
Dependent Claim
67 words
The apparatus as defined in claim 20 including means for modifying the CD4 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD4 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD4 subset population to shift at least one electronic characteristic of said CD4 subset population.
Claim 28
Dependent Claim
67 words
The apparatus as defined in claim 20 including means for modifying the CD8 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD8 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD8 subset population to shift at least one electronic characteristic of said CD8 subset population.
Claim 29
Dependent Claim
67 words
The apparatus as defined in claim 20 including means for modifying the CD2 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD2 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD2 subset population to shift at least one electronic characteristic of said CD2 subset population.
Claim 30
Dependent Claim
67 words
The apparatus as defined in claim 20 including means for modifying the CD20 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD20 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD20 subset population to shift at least one electronic characteristic of said CD20 subset population.
Claim 31
Dependent Claim
51 words
The apparatus as defined in claim 20 wherein said whole blood sample includes a read blood cell population and including means for removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations of interest.
Claim 32
Dependent Claim
64 words
The apparatus as defined in claim 29 wherein removal of said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population; and means for removing said microspheres with said bound red blood cells from said whole blood sample
Claim 33
Dependent Claim
36 words
The apparatus as defined in claim 29 including providing magnetic microspheres and a magnetic field and means for removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 34
Dependent Claim
32 words
The apparatus as defined in claim 29 wherein said means for removing said red blood cell population include providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 35
Dependent Claim
102 words
The apparatus as defined in claim 20 including means for modifying the volume and/or opacity parameters of at least a second white blood cell subset of said white blood cell populations of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing at last one of said modified white blood cell subsets and said selected white blood cell population of interest to determine at least one characteristic of said selected white blood cell population.
Claim 36
Dependent Claim
20 words
The apparatus as defined in claim 33 including means for electronically analyzing both of said modified white blood cell subsets.
Claim 37
Dependent Claim
81 words
The apparatus as defined in claim 33 including means for modifying said two white blood cell subsets by providing microspheres of a first size having a monoclonal antibody bonded thereto specific to said first white blood cell subset and providing microspheres of a second size, different from said first size, having a monoclonal antibody bonded thereto specific to said second white blood cell subset, and means for mixing said microspheres with said sample to bind to said white blood cell populations.
Claim 38
Dependent Claim
101 words
The apparatus as defined in claim 20 including means for modifying said white blood cell population subset by providing a first set of microspheres having a monoclonal antibody bonded thereto specific to said white blood cell subset and means for mixing said microspheres with said sample to bind to said white blood cell populations and then providing a second set of microspheres having a monoclonal antibody bonded thereto specific to said monoclonal antibody bonded onto said first set of microspheres and means for mixing said second set of microspheres with said sample and said first set of microspheres to bind thereto.
Claim 39
Dependent Claim
28 words
The apparatus as defined in claim 29 including means for modifying said white blood cell population subset and means for removing said red blood cell population substantially simultaneously.
Claim 40
Dependent Claim
27 words
The apparatus as defined in claim 29 including means for modifying said white blood cell population subset and means for removing said red blood cell population sequentially.
Claim 41
Independent Claim
144 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: modifying the volume and opacity parameters of at least one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 42
Independent Claim
144 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: modifying the volume and opacity parameters of at least one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 43
Dependent Claim
32 words
The method as defined in claim 39 including subtracting at least one white blood cell population from said white blood cell populations prior to analyzing said modified white blood cell population subset.
Claim 44
Dependent Claim
75 words
The method as defined in claim 40 including subtracting said white blood cell population by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population and mixing said magnetic microspheres with said sample to bind to said white blood cell population and removing said white blood cell population by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 45
Dependent Claim
32 words
The method as defined in claim 39 including subtracting at least the neutrophil and eosinophil populations from said white blood cell population prior to analyzing said modified white blood cell population subset.
Claim 46
Dependent Claim
75 words
The method as defined in claim 42 including subtracting said neutrophil and eosinophil populations by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said neutrophil and eosinophil populations and mixing said magnetic microspheres with said sample to bind to said neutrophil and eosinophil populations and removing said neutrophil and eosinophil populations by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 47
Dependent Claim
63 words
The method as defined in claim 39 including modifying the CD4 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD4 white blood cell population subset and mixing said microspheres with said sample to bind to said CD4 subset population to shift at least one electronic characteristic of said CD4 subset population.
Claim 48
Dependent Claim
63 words
The method as defined in claim 39 including modifying the CD8 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD8 white blood cell population subset and mixing said microspheres with said sample to bind to said CD8 subset population to shift at least one electronic characteristic of said CD8 subset population.
Claim 49
Dependent Claim
63 words
The method as defined in claim 39 including modifying the CD2 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD2 white blood cell population subset and mixing said microspheres with said sample to bind to said CD2 subset population to shift at least one electronic characteristic of said CD2 subset population.
Claim 50
Dependent Claim
63 words
The method as defined in claim 39 including modifying the CD20 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD20 white blood cell population subset and mixing said microspheres with said sample to bind to said CD20 subset population to shift at least one electronic characteristic of said CD20 subset population.
Claim 51
Dependent Claim
48 words
The method as defined in claim 39 wherein said whole blood sample includes a red blood cell population and removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations of interest.
Claim 52
Dependent Claim
60 words
The method as defined in claim 48 wherein removal of said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population; and removing said microspheres with said bound red blood cells from said whole blood sample.
Claim 53
Dependent Claim
34 words
The method as defined in claim 49 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 54
Dependent Claim
29 words
The method as defined in claim 48 wherein removing said red blood cell population includes providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 55
Dependent Claim
98 words
The method as defined in claim 39 including modifying the volume and/or opacity parameters of at least a second white blood cell subset of said white blood cell populations of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell populations subset, said microspheres being substantially smaller than said cells; and electronically analyzing at least one of said modified white blood cell subsets and said selected white blood cell population of interest to determine at least one characteristic of said selected white blood cell population.
Claim 56
Dependent Claim
18 words
The method as defined in claim 52 including electronically analyzing both of said modified white blood cell subsets.
Claim 57
Dependent Claim
77 words
The method as defined in claim 52 including modifying said two white blood cell subsets by providing microspheres of a first size having a monoclonal antibody bonded thereto specific to said first white blood cell subset and providing microspheres of a second size, different from said first size, having a monoclonal antibody bonded thereto specific to said second white blood cell subset, and mixing said microspheres with said sample to bind to said white blood cell populations.
Claim 58
Dependent Claim
95 words
The method as defined in claim 39 including modifying said white blood cell population subset by providing a first set of microspheres having a monoclonal antibody bonded thereto specific to said white blood cell subset and mixing said microspheres with said sample to bind to said white blood cell populations and then providing a second set of microspheres having a monoclonal antibody bonded thereto specific to said monoclonal antibody bonded onto said first set of microspheres and mixing said second set of microspheres with said sample and said first set of microspheres to bind thereto.
Claim 59
Dependent Claim
24 words
The method as defined in claim 48 including modifying said white blood cell population subset and removing said red blood cell population substantially simultaneously.
Claim 60
Dependent Claim
23 words
The method as defined in claim 48 including modifying said white blood cell population subset and removing said red blood cell population sequentially.
Claim 61
Independent Claim
148 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: means for modifying the volume and opacity parameters of at last one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 62
Independent Claim
148 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of the white blood cell populations further having at least one subset, comprising: means for modifying the volume and opacity parameters of at last one white blood cell population subset of said white blood cell population of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing said modified white blood cell population subset and said selected white blood cell population of interest with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said selected white blood cell population.
Claim 63
Dependent Claim
34 words
The apparatus as defined in claim 58 including means for subtracting at least one white blood cell population from said white blood cell poulations prior to analyzing said modified white blood cell population subset.
Claim 64
Dependent Claim
81 words
The apparatus as defined in claim 59 including means for subtracting said white blood cell population by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population and means for mixing said magnetic microspheres with said sample to bind to said white blood cell population and means for removing said white blood cell population by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 65
Dependent Claim
34 words
The apparatus as defined in claim 58 including means for subtracting at least the neutrophil and eosinophil populations from said white blood cell population prior to analyzing said modified white blood cell population subset.
Claim 66
Dependent Claim
79 words
The apparatus as defined in claim 61 including means for subtracting said neutrophil and eosinophil populations by providing magnetic microspheres having a monoclonal antibody bonded thereto specific to said neutrophil and eosinophil populations and means for mixing said magnetic microspheres with said sample to bind to said neutrophil and eosinophil populations and removing said neutrophil and eosinophil populations by removing at least a portion of the remainder of said sample while attracting said magnetic microspheres within a magnetic field.
Claim 67
Dependent Claim
67 words
The apparatus as defined in claim 58 including means for modifying the CD4 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD4 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD4 subset population to shift at least one electronic characteristic of said CD4 subset population.
Claim 68
Dependent Claim
67 words
The apparatus as defined in claim 58 including means for modifying the CD8 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD8 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD8 subset population to shift at least one electronic characteristic of said CD8 subset population.
Claim 69
Dependent Claim
67 words
The apparatus as defined in claim 58 including means for modifying the CD2 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD2 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD2 subset population to shift at least one electronic characteristic of said CD2 subset population.
Claim 70
Dependent Claim
67 words
The apparatus as defined in claim 58 including means for modifying the CD20 white blood cell population subset by providing microspheres having a specific monoclonal antibody bonded thereto which is specific to said CD20 white blood cell population subset and means for mixing said microspheres with said sample to bind to said CD20 subset population to shift at least one electronic characteristic of said CD20 subset population.
Claim 71
Dependent Claim
51 words
The apparatus as defined in claim 58 wherein said whole blood sample includes a red blood cell population and including means for removing the red blood cell population from said sample without significantly adversely affecting relevant qualities and/or quantities of at least one of said white blood cell populations of interest.
Claim 72
Dependent Claim
64 words
The apparatus as defined in claim 67 wherein removal of said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population; and means for removing said microspheres with said bound red blood cells from said whole blood sample.
Claim 73
Dependent Claim
36 words
The apparatus as defined in claim 68 including providing magnetic microspheres and a magnetic field and means for removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 74
Dependent Claim
31 words
The apparatus as defined in claim 67 wherein said means for removing said red blood cell population include providing red blood cell lyse to substantially eliminate said red blood cell population.
Claim 75
Dependent Claim
102 words
The apparatus as defined in claim 58 including means for modifying the volume and/or opacity parameters of at least a second white blood cell subset of said white blood cell populations of interest by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing at least one of said modified white blood cell subsets and said selected white blood cell population of interest to determine at least one characteristic of said selected white blood cell population.
Claim 76
Dependent Claim
20 words
The apparatus as defined in claim 71 including means for electronically analyzing both of said modified white blood cell subsets.
Claim 77
Dependent Claim
81 words
The apparatus as defined in claim 71 including means for modifying said two white blood cell subsets by providing microspheres of a first size having a monoclonal antibody bonded thereto specific to said first white blood cell subset and providing microspheres of a second size, different from said first size, having a monoclonal antibody bonded thereto specific to said second white blood cell subset, and means for mixing said microspheres with said sample to bind to said white blood cell populations.
Claim 78
Dependent Claim
101 words
The apparatus as defined in claim 58 including means for modifying said white blood cell population subset by providing a first set of microspheres having a monoclonal antibody bonded thereto specific to said white blood cell subset and means for means for mixing said microspheres with said sample to bind to said white blood cell populations and then providing a second set of microspheres having a monoclonal antibody bonded thereto specific to said monoclonal antibody bonded onto said first set of microspheres and mixing said second set of microspheres with said sample and said first set of microspheres to bind thereto.
Claim 79
Dependent Claim
28 words
The apparatus as defined in claim 67 including means for modifying said white blood cell population subset and means for removing said red blood cell population substantially simultaneously.
Claim 80
Dependent Claim
27 words
The apparatus as defined in claim 67 including means for modifying said white blood cell population subset and means for removing said red blood cell population sequentially.
Claim 81
Independent Claim
139 words
A method of obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphocytes with at least two electronic sensing parameters utilizing Coulter sensing techniques; subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population; electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques; and comparing said two counts to obtain a count of said white blood cell population of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 82
Independent Claim
139 words
A method of obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphocytes with at least two electronic sensing parameters utilizing Coulter sensing techniques; subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population; electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques; and comparing said two counts to obtain a count of said white blood cell population of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 83
Dependent Claim
41 words
The method as defined in claim 77 wherein said whole blood sample includes a red blood cell population and removing said red blood cell population from said sample without adversely affecting relevant qualities and/or quantities of said white blood cell populations.
Claim 84
Dependent Claim
60 words
The method as defined in claim 78 wherein removal of said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population; and removing said microspheres with said bound red blood cells from said whole blood sample.
Claim 85
Dependent Claim
34 words
The method as defined in claim 79 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 86
Dependent Claim
32 words
The method as defined in claim 79 including rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds.
Claim 87
Dependent Claim
94 words
The method as defined in claim 78 wherein removing said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population and providing a red blood cell lyse with said microspheres to eliminate a portion of said red blood cell populations to decrease the number of microspheres necessary to remove said red blood cell populations; and removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 88
Dependent Claim
34 words
The method as defined in claim 82 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 89
Dependent Claim
38 words
The method as defined in claim 82 including rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds and to provide and lysing action.
Claim 90
Dependent Claim
29 words
The method as defined in claim 78 wherein removing said red blood cell population includes providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 91
Dependent Claim
47 words
The method as defined in claim 77 wherein subtracting the neutrophil population includes providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and mixing said microspheres with said sample to bind to said neutrophil population to shift at least one electronic characteristic of said neutrophil population.
Claim 92
Dependent Claim
49 words
The method as defined in claim 77 wherein subtracting the neutrophil populations includes providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and mixing said microspheres with said sample to bind to said neutrophil population; and removing said microspheres with said neutrophil population bound thereto from said sample.
Claim 93
Dependent Claim
32 words
The method as defined in claim 87 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said neutrophil population while attracting magnetic microspheres within said magnetic field.
Claim 94
Dependent Claim
28 words
The method as defined in claim 87 including rapidly mixing said microspheres with said sample to bind said neutrophil population to said microspheres in less than sixty seconds.
Claim 95
Independent Claim
147 words
An apparatus for obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphoytes with at least two electronic sensing parameters utilizing Coulter sensing techniques; means for subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population; means for electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques; and means for comparing said two counts to obtain a count of said white blood cell population of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 96
Independent Claim
147 words
An apparatus for obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphoytes with at least two electronic sensing parameters utilizing Coulter sensing techniques; means for subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population; means for electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques; and means for comparing said two counts to obtain a count of said white blood cell population of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 97
Dependent Claim
43 words
The apparatus as defined in claim 90 wherein said whole blood sample includes a red blood cell population and means for removing said red blood cell population from said sample without adversely affecting relevant qualities and/or quantities of said white blood cell populations.
Claim 98
Dependent Claim
64 words
The apparatus as defined in claim 91 wherein removal of said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population; and means for removing said microspheres with said bound red blood cells from said whole blood sample.
Claim 99
Dependent Claim
34 words
The apparatus as defined in claim 92 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 100
Dependent Claim
34 words
The apparatus as defined in claim 92 including means for rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds.
Claim 101
Dependent Claim
101 words
The apparatus as defined in claim 91 wherein said means for removing said red blood cell population include providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population and providing a red blood cell lyse with said microspheres to eliminate a portion of said red blood cell populations to decrease the number of microspheres necessary to remove said red blood cell populations; and means for removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 102
Dependent Claim
34 words
The apparatus as defined in claim 95 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 103
Dependent Claim
40 words
The apparatus as defined in claim 95 including means for rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds and to provide said lysing action.
Claim 104
Dependent Claim
32 words
The apparatus as defined in claim 91 wherein said means for removing said red blood cell population includes providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 105
Dependent Claim
52 words
The apparatus as defined in claim 90 wherein said means for subtracting the neutrophil population include providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and means for mixing said microspheres with said sample to bind to said neutrophil population to shift at least one electronic characteristic of said neutrophil population.
Claim 106
Dependent Claim
56 words
The apparatus as defined in claim 90 wherein said means for subtracting the neutrophil populations include providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and means for mixing said microspheres with said sample to bind to said neutrophil population; and means for removing said microspheres with said neutrophil population bound thereto from said sample.
Claim 107
Dependent Claim
33 words
The apparatus as defined in claim 100 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said neutrophil population while attracting said magnetic microspheres within said magnetic field.
Claim 108
Dependent Claim
30 words
The apparatus as defined in claim 100 including means for rapidly mixing said microspheres with said sample to bind said neutrophil population to said microspheres in less than sixty seconds.
Claim 109
Independent Claim
178 words
A method of obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphocytes in a first portion of said sample with at least two electronic sensing parameters utilizing Coulter sensing techniques; subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population from a second portion of said sample without adversely affecting the relevant qualities and/or quantities of said remaining white blood cell populations; electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils in said second portion with at least two electronic sensing parameters utilizing Coulter sensing techniques; and comparing said two counts from said first and second portions to obtain a count of said white blood cell populations of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 110
Independent Claim
178 words
A method of obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphocytes in a first portion of said sample with at least two electronic sensing parameters utilizing Coulter sensing techniques; subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population from a second portion of said sample without adversely affecting the relevant qualities and/or quantities of said remaining white blood cell populations; electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils in said second portion with at least two electronic sensing parameters utilizing Coulter sensing techniques; and comparing said two counts from said first and second portions to obtain a count of said white blood cell populations of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 111
Dependent Claim
66 words
The method as defined in claim 103 wherein said whole blood sample includes a red blood cell population and removing said red blood cell population from said sample without adversely affecting relevant qualities and/or quantities of said white blood cell populations prior to the counting of said first portion and also removing said red blood cell population from said second portion prior to the counting thereof.
Claim 112
Dependent Claim
67 words
The method as defined in claim 104 wherein removing said red blood cell population from at least one of said portions includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population; and removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 113
Dependent Claim
34 words
The method as defined in claim 105 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 114
Dependent Claim
32 words
The method as defined in claim 105 including rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds.
Claim 115
Dependent Claim
101 words
The method as defined in claim 104 wherein removing said red blood cell population from at least one of said portions includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population and providing a red blood cell lyse with said microspheres to eliminate a portion of said red blood cell populations to decrease the number of microspheres necessary to remove said red blood cell populations; and removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 116
Dependent Claim
34 words
The method as defined in claim 108 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 117
Dependent Claim
39 words
The method as defined in claim 108 including rapidly mixing said microspheres with said whole blood sample portion to bind said red blood cell population to said microspheres in less than sixty seconds and to provide said lysing action.
Claim 118
Dependent Claim
36 words
The method as defined in claim 104 wherein removing said red blood cell population from at least one of said portions includes providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 119
Dependent Claim
53 words
The method as defined in claim 103 wherein subtracting the neutrophil population in said second portion includes providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said neutrophil population to shift at least one electronic characteristic of said neutrophil population.
Claim 120
Dependent Claim
55 words
The method as defined in claim 103 wherein subtracting the neutrophil population in said second portion includes providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said neutrophil population; and removing said microspheres with said neutrophil population bound thereto from said sample.
Claim 121
Dependent Claim
33 words
The method as defined in claim 113 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said neutrophil population while attracting said magnetic microspheres within said magnetic field.
Claim 122
Dependent Claim
30 words
The method as defined in claim 113 including rapidly mixing said microspheres with said whole blood sample to bind said neutrophil population to said microspheres in less than sixty seconds.
Claim 123
Independent Claim
186 words
An apparatus for obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphocytes in a first portion of said sample with at least two electronic sensing parameters utilizing Coulter sensing techniques; means for subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population from a second portion of said sample without adversely affecting the relevant qualities and/or quantities of said remaining white blood cell populations; means for electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils in said second portion with at least two electronic sensing parameters utilizing Coulter sensing techniques; and means for comparing said two counts from said first and second portions to obtain a count of said white blood cell populations of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 124
Independent Claim
186 words
An apparatus for obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for electronically counting at least said white blood cell populations of granulocytes, monocytes and lymphocytes in a first portion of said sample with at least two electronic sensing parameters utilizing Coulter sensing techniques; means for subtracting the neutrophil population contribution from said white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population from a second portion of said sample without adversely affecting the relevant qualities and/or quantities of said remaining white blood cell populations; means for electronically counting at least said remaining white blood cell populations of monocytes, lymphocytes, eosinophils and basophils in said second portion with at least two electronic sensing parameters utilizing Coulter sensing techniques; and means for comparing said two counts from said first and second portions to obtain a count of said white blood cell populations of neutrophils and thereby obtaining at least a five-part white blood cell differential.
Claim 125
Dependent Claim
69 words
The apparatus as defined in claim 116 wherein said whole blood sample includes a red blood cell population and including means for removing said red blood cell population from said sample without adversely affecting relevant qualities and/or quantities of said white blood cell populations prior to the counting of said first portion and also removing said red blood cell population from said second portion prior to the coating thereof.
Claim 126
Dependent Claim
74 words
The apparatus as defined in claim 112 wherein said means for removing said red blood cell population from at least one of said portions include providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population; and means for removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 127
Dependent Claim
34 words
The apparatus as defined in claim 118 including providing magnetic microspheres an a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 128
Dependent Claim
34 words
The apparatus as defined in claim 118 including means for rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds.
Claim 129
Dependent Claim
108 words
The apparatus as defined in claim 117 wherein said means for removing said red blood cell population from at least one of said portions include providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population and providing a red blood cell lyse with said microspheres to eliminate a portion of said red blood cell populations to decrease the number of microspheres necessary to remove said red blood cell populations; and means for removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 130
Dependent Claim
34 words
The apparatus as defined in claim 121 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 131
Dependent Claim
39 words
The apparatus as defined in claim 121 including means for rapidly mixing said microspheres with said whole blood sample portion to red blood cell population to said microspheres in less than sixty seconds and to provide said lysing action.
Claim 132
Dependent Claim
39 words
The apparatus as defined in claim 117 wherein said means for removing said red blood cell population from at least one of said portions include providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 133
Dependent Claim
58 words
The apparatus as defined in claim 116 wherein said means for subtracting the neutrophil population in said second portion include providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said neutrophil population to shift at least one electronic characteristic of said neutrophil population.
Claim 134
Dependent Claim
62 words
The apparatus as defined in claim 118 wherein said means for subtracting the neutrophil population in said second portion include providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said neutrophil population; and means for removing said microspheres with said neutrophil population bound thereto from said sample.
Claim 135
Dependent Claim
33 words
The apparatus as defined in claim 126 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said neutrophil population while attracting said magnetic microspheres within said magnetic field.
Claim 136
Dependent Claim
32 words
The apparatus as defined in claim 126 including means for rapidly mixing said microspheres with said whole blood sample to bind said neutrophil population to said microspheres in less than sixty seconds.
Claim 137
Independent Claim
111 words
A method of obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: shifting the neutrophil population characteristic contribution with respect to the other white blood cell populations by binding microspheres having monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population, said microspheres being substantially smaller than said neutrophil population cells; and electronically counting at least said white blood cell populations of monocytes, lymphocytes, neutrophils, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques and thereby obtaining at least a five-part white blood cell differential.
Claim 138
Independent Claim
111 words
A method of obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: shifting the neutrophil population characteristic contribution with respect to the other white blood cell populations by binding microspheres having monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population, said microspheres being substantially smaller than said neutrophil population cells; and electronically counting at least said white blood cell populations of monocytes, lymphocytes, neutrophils, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques and thereby obtaining at least a five-part white blood cell differential.
Claim 139
Dependent Claim
41 words
The method as defined in claim 129 wherein said whole blood sample includes a red blood cell population and removing said red blood cell population from said sample without adversely affecting relevant qualities and/or quantities of said white blood cell populations.
Claim 140
Dependent Claim
60 words
The method as defined in claim 130 wherein removing said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population; and removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 141
Dependent Claim
34 words
The method as defined in claim 131 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres with said magnetic field.
Claim 142
Dependent Claim
32 words
The method as defined in claim 131 including rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds.
Claim 143
Dependent Claim
94 words
The method as defined in claim 130 wherein removing said red blood cell population includes providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and mixing said microspheres with said whole blood sample to bind to said red blood cell population and providing a red blood cell lyse with said microspheres to eliminate a portion of said red blood cell populations to decrease the number of microspheres necessary to remove said red blood cell population; and removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 144
Dependent Claim
34 words
The method as defined in claim 134 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 145
Dependent Claim
38 words
The method as defined in claim 134 including rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds and to provide said lysing action.
Claim 146
Dependent Claim
29 words
The method as defined in claim 130 wherein removing said red blood cell population includes providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 147
Dependent Claim
47 words
The method as defined in claim 129 wherein shifting the neutrophil population includes providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and mixing said microspheres with said sample to bind to said neutrophil population to shift at least one electronic characteristic of said neutrophil population.
Claim 148
Dependent Claim
28 words
The method as defined in claim 138 including rapidly mixing said microspheres with said sample to bind said neutrophil population to said microspheres in less than sixty seconds.
Claim 149
Independent Claim
116 words
An apparatus for obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for shifting the neutrophil population characteristic contribution with respect to the other white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population, said microspheres being substantially smaller than said neutrophil population cells; and means for electronically counting at least said white blood cell populations of monocytes, lymphocytes, neutrophils, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques and thereby obtaining at least a five-part white blood cell differential.
Claim 150
Independent Claim
116 words
An apparatus for obtaining a multi-part white blood cell population differential from at least a portion of a whole blood sample having at least white blood cell populations therein, comprising: means for shifting the neutrophil population characteristic contribution with respect to the other white blood cell populations by binding microspheres having a monoclonal antibody bonded thereto specific to said neutrophil population to said neutrophil population, said microspheres being substantially smaller than said neutrophil population cells; and means for electronically counting at least said white blood cell populations of monocytes, lymphocytes, neutrophils, eosinophils and basophils with at least two electronic sensing parameters utilizing Coulter sensing techniques and thereby obtaining at least a five-part white blood cell differential.
Claim 151
Dependent Claim
43 words
The apparatus as defined in claim 140 wherein said whole blood sample includes a red blood cell population and means for removing said red blood cell population from said sample without adversely affecting relevant quantities and/or quantities of said white blood cell populations.
Claim 152
Dependent Claim
67 words
The apparatus as defined in claim 141 wherein said means for removing said red blood cell population include providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population; and means for removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 153
Dependent Claim
34 words
The apparatus as defined in claim 142 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 154
Dependent Claim
34 words
The apparatus as defined in claim 142 including means for rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds.
Claim 155
Dependent Claim
101 words
The apparatus as defined in claim 141 wherein said means for removing said red blood cell population include providing microspheres having a red blood cell specific monoclonal antibody bonded thereto and means for mixing said microspheres with said whole blood sample to bind to said red blood cell population and providing a red blood cell lyse with said microspheres to eliminate a portion of said red blood cell populations to decrease the number of microspheres necessary to remove said red blood cell population; and means for removing said microspheres with said red blood cells bound thereto from said whole blood sample.
Claim 156
Dependent Claim
34 words
The apparatus as defined in claim 145 including providing magnetic microspheres and a magnetic field and removing said microspheres by removing said red blood cells while attracting said magnetic microspheres within said magnetic field.
Claim 157
Dependent Claim
40 words
The apparatus as defined in claim 145 including means for rapidly mixing said microspheres with said whole blood sample to bind said red blood cell population to said microspheres in less than sixty seconds and to provide said lysing action.
Claim 158
Dependent Claim
32 words
The apparatus as defined in claim 141 wherein said means for removing said red blood cell population include providing a red blood cell lyse to substantially eliminate said red blood cell population.
Claim 159
Dependent Claim
52 words
The apparatus as defined in claim 140 wherein said mans for shifting the neutrophil population include providing microspheres having a neutrophil specific monoclonal antibody bonded thereto and mans for mixing said microspheres with said sample to bind to said neutrophil population to shift at least one electronic characteristic of said neutrophil population.
Claim 160
Dependent Claim
30 words
The apparatus as defined in claim 149 including means for rapidly mixing said microspheres with said sample to bind said neutrophil population to said microspheres in less than sixty seconds.
Claim 161
Independent Claim
132 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of said white blood cell populations further having at least two subsets, comprising: subtracting at least one subset contribution from its specific white blood cell population by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and electronically analyzing said subtracted white blood cell population with at least two electronic sensing parameters utilizing Coulter sensing techniques subset and said selected white blood cell population to determine at least one characteristic of said selected white blood cell population.
Claim 162
Independent Claim
132 words
A method of obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of said white blood cell populations further having at least two subsets, comprising: subtracting at least one subset contribution from its specific white blood cell population by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and electronically analyzing said subtracted white blood cell population with at least two electronic sensing parameters utilizing Coulter sensing techniques subset and said selected white blood cell population to determine at least one characteristic of said selected white blood cell population.
Claim 163
Dependent Claim
41 words
The method as defined in claim 151 wherein said whole blood sample includes a red blood cell population and removing said red blood cell population from said sample without adversely affecting relevant quantities and/or quantities of said white blood cell populations.
Claim 164
Independent Claim
136 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of said white blood cell populations further having at least two subsets, comprising: means for subtracting at least one subset contribution from its specific white blood cell population by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing said subtracted white blood cell population with at least two electronic sensing parameters utilizing Coulter sensing techniques subset and said selected white blood cell population to determine at least one characteristic of said selected white blood cell population.
Claim 165
Independent Claim
136 words
An apparatus for obtaining at least one white blood cell population analysis from at least a portion of a whole blood sample having at least white blood cell populations therein, at least one of said white blood cell populations further having at least two subsets, comprising: means for subtracting at least one subset contribution from its specific white blood cell population by binding microspheres having a monoclonal antibody bonded thereto specific to said white blood cell population subset to said white blood cell population subset, said microspheres being substantially smaller than said cells; and means for electronically analyzing said subtracted white blood cell population with at least two electronic sensing parameters utilizing Coulter sensing techniques subset and said selected white blood cell population to determine at least one characteristic of said selected white blood cell population.
Claim 166
Dependent Claim
43 words
The apparatus as defined in claim 153 wherein said whole blood sample includes a red blood cell population and means for removing said red blood cell population from said sample without adversely affecting relevant quantities and/or quantities of said white blood cell populations.
Claim 167
Independent Claim
109 words
A method of obtaining a classification of a sample of cells or formed bodies, comprising: modifying the volume and/or opacity parameters of at least one population of the cells or formed bodies of interest by binding microspheres having a monoclonal antibody bonded thereto specific to cells or formed bodies to cells or formed bodies, said microspheres being substantially smaller than said cells or said formed bodies; and electronically analyzing said modified cells or formed bodies of interest and the remaining cells or formed bodies with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said cells or formed bodies of interest.
Claim 168
Independent Claim
109 words
A method of obtaining a classification of a sample of cells or formed bodies, comprising: modifying the volume and/or opacity parameters of at least one population of the cells or formed bodies of interest by binding microspheres having a monoclonal antibody bonded thereto specific to cells or formed bodies to cells or formed bodies, said microspheres being substantially smaller than said cells or said formed bodies; and electronically analyzing said modified cells or formed bodies of interest and the remaining cells or formed bodies with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said cells or formed bodies of interest.
Claim 169
Dependent Claim
24 words
The method as defined in claim 155 including modifying the volume and opacity parameters of at least one population of the cells of interest.
Claim 170
Dependent Claim
25 words
The method as defined in claim 155 including modifying the volume and opacity parameters of at least one population of the formed bodies of interest.
Claim 171
Independent Claim
112 words
An apparatus for obtaining a classification of a sample of cells or formed bodies, comprising: means for modifying the volume and/or opacity parameters of at least one population of the cells or formed bodies of interest by binding microspheres having a monoclonal antibody bonded thereto specific to cells or formed bodies to cells or formed bodies, said microspheres being substantially smaller than said cells or formed bodies; and means for electronically analyzing said modified cells or formed bodies of interest and the remaining cells or formed bodies with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said cells or formed bodies of interest.
Claim 172
Independent Claim
112 words
An apparatus for obtaining a classification of a sample of cells or formed bodies, comprising: means for modifying the volume and/or opacity parameters of at least one population of the cells or formed bodies of interest by binding microspheres having a monoclonal antibody bonded thereto specific to cells or formed bodies to cells or formed bodies, said microspheres being substantially smaller than said cells or formed bodies; and means for electronically analyzing said modified cells or formed bodies of interest and the remaining cells or formed bodies with at least two electronic sensing parameters utilizing Coulter sensing techniques to determine at least one characteristic of said cells or formed bodies of interest.
Claim 173
Dependent Claim
26 words
The apparatus as defined in claim 158 including means for modifying the volume and opacity parameters of at least one population of the cells of interest.
Claim 174
Dependent Claim
27 words
The apparatus as defined in claim 158 including means for modifying the volume and opacity parameters of at least one population of the formed bodies of interest.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-07-26 | 1997-01-25 | 1997-07-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-07-26 | 2001-01-25 | 2001-07-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-07-26 | 2005-01-25 | 2005-07-27 | $7700.00 | Window Closed / Lapsed |