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US5227301A
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Immortalized bovine mannary epithelial cell line
Assignee: Royal Institution for the Advancement of Learning
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Filing: 1989-11-03
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Granted: 1993-07-13
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Expires: 2009-11-03
Abstract
Abstract The present invention relates to a bovine immortalized mammary epithelial cell line which has normal physiological responses in that it produces milk constituents which comprises α- and β-casein and lactose. There is provided, using the cell line of the present invention a method of `in vitro` studying lactation. There is provided a method of `in vitro` screening for gene expression of DNA constructs for transgenic cows, since the cell line of the present invention is a bovine one. The cell line can be further used in a method for indefinitely expressing foreign genes. The cell line of the present invention has been deposited at the ATCC under the accession number CRL 10274.
Inventors
- 👤 Jeffrey D. Turner
- 👤 Hung Huynh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 23:17
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0631; C12N15/00; C12N2510/00; C12N2510/02; C12N2510/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
43 words
An immortalized bovine mammary epithelial cell line prepared by the transfection of primary bovine mammary epithelial cells with the SV40 large T antigen, said cell line having normal physiological responses such that, under hormonal stimulation, produces milk constituents comprising α-and β-casein and lactose.
Claim 2
Dependent Claim
43 words
An immortalized bovine mammary epithelial cell line prepared by the transfection of primary bovine mammary epithelial cells with the SV40 large T antigen, said cell line having normal physiological responses such that, under hormonal stimulation, produces milk constituents comprising α-and β-casein and lactose.
Claim 3
Dependent Claim
82 words
A method of stimulating the production of certain milk proteins by bovine mammary epithelial cells in vitro which comprises the steps of: (a) incubating the immortalized bovine mammary epithelial cell line of claim 1 in a culture medium, (b) adding to the culture medium one or more lactation hormones to stimulate production of certain milk proteins by said cell line, and (c) measuring the amount of α- and β-casein and lactose produced and secreted into the culture medium by said cell line.
Claim 4
Dependent Claim
89 words
A method of in vitro screening for foreign gene expression in bovines which comprises the steps of: a) providing the immortalized cell line of claim 1; b) transfecting the cells of step a) with a foreign DNA construct comprising a bovine casein promoter and a foreign gene wherein the mammary gland is the target organ for said foreign gene expression; and c) assaying the transfected cells of step b) for said foreign gene expression; thereby determining the suitability of the foreign DNA construct prior to making a transgenic bovine.
Claim 5
Dependent Claim
13 words
The cell line according to claim 1 having the ATCC number, CRL 10274.
References Cited (Backward Citations)
US4808532A
Cited
US5026637A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-07-12 | 1997-01-11 | 1997-07-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-07-12 | 2001-01-11 | 2001-07-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-07-12 | 2005-01-11 | 2005-07-13 | $7700.00 | Window Closed / Lapsed |