Patent US5225348A successfully refreshed from Google Patents.
US5225348A
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DNA fragment and expression plasmid containing the DNA fragment
Assignee: Mochida Pharmaceutical Co Ltd
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Filing: 1989-12-08
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Granted: 1993-07-06
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Expires: 2009-12-08
Abstract
Abstract A DNA fragment containing a promoter region for human polypeptide chain elongation factor-1α, its base sequence and expression plasmids containing the DNA fragment having high applicability to a wide range of host cells with high expression capacity. These expression plasmids can be maintained stably for at least one month in mammalian host cells. Such features of the expression plasmids may render possible the efficient production of various kinds of useful physiologically active substances for a long period using wide range of mammalian cells as the host.
Inventors
- 👤 Shigekazu Nagata
- 👤 Sumio Sugano
- 👤 Dong W. Kim
- 👤 Taichi Uetsuki
- 👤 Yoshito Kaziro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:02
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
12 words
An essentially pure DNA fragment represented by the following sequence (I): ##STR2##
Claim 2
Dependent Claim
12 words
An essentially pure DNA fragment represented by the following sequence (I): ##STR2##
Claim 3
Dependent Claim
12 words
The essentially pure DNA fragment represented by the following sequence (II): ##STR3##
Claim 4
Dependent Claim
12 words
The essentially pure DNA fragment represented by the following sequence (II): ##STR3##
Claim 5
Dependent Claim
12 words
The essentially pure DNA fragment represented by the following sequence (III): ##STR4##
Claim 6
Dependent Claim
12 words
The essentially pure DNA fragment represented by the following sequence (III): ##STR4##
Claim 7
Dependent Claim
12 words
The essentially pure DNA fragment represented by the following sequence (IV): ##STR5##
Claim 8
Dependent Claim
12 words
The essentially pure DNA fragment represented by the following sequence (IV): ##STR5##
Claim 9
Dependent Claim
11 words
An expression plasmid containing the DNA fragment according to claim 1.
Claim 10
Dependent Claim
12 words
An expression plasmid according to claim 5, wherein the plasmid is pEF321-CAT.
Claim 11
Dependent Claim
11 words
An expression plasmid containing a DNA fragment according to claim 2.
Claim 12
Dependent Claim
12 words
An expression plasmid according to claim 7, wherein the plasmid is pEF220-CAT.
Claim 13
Dependent Claim
11 words
An expression plasmid containing a DNA fragment according to claim 3.
Claim 14
Dependent Claim
12 words
An expression plasmid according to claim 9, wherein the plasmid is pEF223-CAT.
Claim 15
Dependent Claim
11 words
An expression plasmid containing a DNA fragment according to claim 4.
Claim 16
Dependent Claim
12 words
An expression plasmid according to claim 11, wherein the plasmid is pEF204-CAT.
Claim 17
Dependent Claim
47 words
An essentially pure DNA fragment comprising all or a portion of the DNA fragment represented by the following sequence (I): ##STR6## said portion containing the promoter for the human polypeptide chain elongation factor-1α gene which when operably linked to a heterologous structural gene regulates the transcription thereof.
Claim 18
Dependent Claim
47 words
An essentially pure DNA fragment comprising all or a portion of the DNA fragment represented by the following sequence (I): ##STR6## said portion containing the promoter for the human polypeptide chain elongation factor-1α gene which when operably linked to a heterologous structural gene regulates the transcription thereof.
Claim 19
Dependent Claim
11 words
An expression plasmid containing the DNA fragment according to claim 13.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-07-05 | 1997-01-04 | 1997-07-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-07-05 | 2001-01-04 | 2001-07-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-07-05 | 2005-01-04 | 2005-07-06 | $7700.00 | Window Closed / Lapsed |