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US5223420A
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Elastin-based product, a procedure for its preparation and its biological applications; in particular as biomaterials and artificial supports
Assignee: Institut National de la Santé et de la Recherche Médicale
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Filing: 1986-03-03
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Granted: 1993-06-29
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Expires: 2006-03-03
Abstract
Abstract PCT No. PCT/FR86/00068 Sec. 371 Date Oct. 29, 1986 Sec. 102(e) Date Oct. 29, 1986 PCT Filed Mar. 3, 1986 PCT Pub. No. WO86/05097 PCT Pub. Date Sep. 12, 1986.The subject of the invention is an elastin-based product consisting of an adduct containing elastin and at least one protein which can be coagulated and/or activated by thrombin.
Inventors
- 👤 Michel Rabaud
- 👤 Francoise Lefebure Clement
- 👤 Henri Bricaud
- 👤 Roland Schmidthaeusler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:49
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3604; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/39; A61L27/14; A61L27/22; A61L27/227; A61L27/3641; A61L27/3645
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
15 words
An elastin containing composition comprising fibronectin and wherein elastin is covalently bound to fibrin monomers.
Claim 2
Dependent Claim
15 words
An elastin containing composition comprising fibronectin and wherein elastin is covalently bound to fibrin monomers.
Claim 3
Dependent Claim
19 words
An elastin containing composition consisting essentially of a synthetic compound resulting from the reaction of fibrin monomers with elastin.
Claim 4
Dependent Claim
19 words
An elastin containing composition consisting essentially of a synthetic compound resulting from the reaction of fibrin monomers with elastin.
Claim 5
Dependent Claim
19 words
An elastin containing composition obtained by reacting elastin with cryoglobulins, in the presence of thrombin and Ca ++ .
Claim 6
Dependent Claim
19 words
An elastin containing composition obtained by reacting elastin with cryoglobulins, in the presence of thrombin and Ca ++ .
Claim 7
Dependent Claim
100 words
An elastin containing composition according to claim 3 wherein said cryoglobulins are selected from the group consisting of cryoglobulins corresponding to the lyophilized fraction called antihemophilic A, the latter containing about 10 to 15 g/l of fibrinogen and about 2 to 4 g/l of fibronectin, cryoglobulins obtained during the isolation of concentrated Factor VIII, the latter which contains about 9 to 20 g/l of fibrinogen and about 10 to 14 g/l of fibronectin, and cryoglobulins composed of a lyophilized protein complex called transglutin, which contains about 50 to 90 g/l of fibrinogen and about 8 to 15 g/l of fibronectin.
Claim 8
Dependent Claim
19 words
An elastin containing composition obtained by reacting plasma with elastin, in the presence of thrombin and Ca ++ .
Claim 9
Dependent Claim
19 words
An elastin containing composition obtained by reacting plasma with elastin, in the presence of thrombin and Ca ++ .
Claim 10
Dependent Claim
56 words
An elastin containing composition according to claim 1, further comprising at least one additive selected from the group consisting of collagen, thiourea, cellulose derivatives, calcium alginate, sodium ethylmercrylthiosalicylate, FVIII complex (antihemophilic A), the latter containing about 10 to 15 g/l of fibrinogen and about 2 to 4 g/l of fibronectin, von Willebrand factor, thrombospondin and laminin.
Claim 11
Dependent Claim
18 words
An elastin containing composition according to claim 2, wherein the elastin and fibrin monomers are of human origin.
Claim 12
Dependent Claim
18 words
An elastin containing composition according to claim 2, wherein the elastin and fibrin monomers are of animal origin.
Claim 13
Dependent Claim
27 words
An elastin containing composition consisting essentially of a synthetic compound resulting from the reaction of fibrinogen with elastin, in the presence of thrombin and Ca ++ .
Claim 14
Dependent Claim
27 words
An elastin containing composition consisting essentially of a synthetic compound resulting from the reaction of fibrinogen with elastin, in the presence of thrombin and Ca ++ .
Claim 15
Dependent Claim
23 words
An elastin containing composition according to claim 6, wherein said elastin containing composition is an adduct in the form of a cross-linked network.
Claim 16
Dependent Claim
49 words
A process of preparing an elastin containing composition comprising the steps of: contacting with fibrinogen a suspension of elastin in a buffer containing Ca ++ ; adding thrombin to the resultant mixture to form a reaction mixture; incubating the reaction mixture; and then separating the resulting elastin-fibrin monomers adduct.
Claim 17
Dependent Claim
49 words
A process of preparing an elastin containing composition comprising the steps of: contacting with fibrinogen a suspension of elastin in a buffer containing Ca ++ ; adding thrombin to the resultant mixture to form a reaction mixture; incubating the reaction mixture; and then separating the resulting elastin-fibrin monomers adduct.
Claim 18
Dependent Claim
13 words
A process according to claim 11, wherein said fibrinogen is provided by plasma.
Claim 19
Dependent Claim
13 words
A process according to claim 11, wherein said fibrinogen is provided by cryoglobulins.
Claim 20
Dependent Claim
40 words
A process according to claim 11, comprising the step of pouring said reaction mixture into a mold prior to incubation, and comprising the step of recovering, washing and drying the resulting adduct to enable the use thereof as a biomaterial.
Claim 21
Dependent Claim
61 words
A process according to claim 11 further comprising the step of adding, prior to incubation, at least one additive selected from the group consisting of collagen, thiourea, cellulose derivatives, calcium alginate, sodium ethylmercrylthiosalicylate, FVIII complex (antihemophilic A), the latter containing about 10 to 15 g/l of fibrinogen and about 2 to 4 g/l of fibronectin, von Willebrand factor, thrombospondin and laminin.
Claim 22
Dependent Claim
39 words
The process according to claim 11, wherein said incubation step is carried out at about 37° C. in a buffer of a pH of about 7.4, the Ca ++ content of which is on the order of 2 mM.
Claim 23
Dependent Claim
13 words
An artificial connective tissue comprising the elastin containing composition according to claim 3.
Claim 24
Dependent Claim
13 words
An artificial connective tissue comprising the elastin containing composition according to claim 5.
Claim 25
Dependent Claim
13 words
An artificial connective tissue comprising the elastin containing composition according to claim 2.
Claim 26
Dependent Claim
13 words
An artificial connective tissue comprising the elastin containing composition according to claim 9.
Claim 27
Independent Claim
32 words
A method of preparing an artificial connective tissue which comprises the use of an elastin containing composition consisting essentially of a synthetic compound resulting from the reaction of fibrin monomers with elastin.
Claim 28
Independent Claim
32 words
A method of preparing an artificial connective tissue which comprises the use of an elastin containing composition consisting essentially of a synthetic compound resulting from the reaction of fibrin monomers with elastin.
Claim 29
Independent Claim
25 words
A method of preparing an artificial connective tissue which comprises the use of an elastin containing composition, wherein elastin is covalently bound to fibrin monomers.
Claim 30
Independent Claim
25 words
A method of preparing an artificial connective tissue which comprises the use of an elastin containing composition, wherein elastin is covalently bound to fibrin monomers.
Claim 31
Dependent Claim
15 words
A physical support for cell cultures comprising the elastin containing composition according to claim 2.
Claim 32
Dependent Claim
15 words
A physical support for cell cultures comprising the elastin containing composition according to claim 9.
Claim 33
Dependent Claim
15 words
A physical support for cell cultures comprising the elastin containing composition according to claim 3.
Claim 34
Dependent Claim
15 words
A physical support for cell cultures comprising the elastin containing composition according to claim 5.
Claim 35
Dependent Claim
16 words
Means for incorporating enzymes or enzyme inhibitors comprising the elastin containing composition according to claim 2.
Claim 36
Dependent Claim
16 words
Means for incorporating enzymes or enzyme inhibiting comprising the elastin containing composition according to claim 9.
Claim 37
Dependent Claim
16 words
Means for incorporating enzymes or enzyme inhibitors comprising the elastin containing composition according to claim 3.
Claim 38
Dependent Claim
16 words
Means for incorporating enzymes or enzyme inhibitors comprising the elastin containing composition according to claim 5.
Claim 39
Dependent Claim
17 words
A kit for cell culture comprising a receptacle containing the elastin containing composition according to claim 2.
Claim 40
Dependent Claim
25 words
A kit for tissue repair comprising a storage envelope containing the elastin containing composition according to claim 2 and a container containing a biological adhesive.
References Cited (Backward Citations)
US4614794A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1996-06-28 | 1996-12-28 | 1997-06-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-06-28 | 2000-12-28 | 2001-06-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-06-28 | 2004-12-28 | 2005-06-29 | $7700.00 | Window Closed / Lapsed |