US5216131A
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US
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Lymphocyte homing receptors
Assignee: Genentech Inc
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Filing: 1991-10-31
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Granted: 1993-06-01
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Expires: 2011-10-31
Abstract
Abstract DNA isolates coding for the lymphocyte homing receptor and methods of obtaining such DNA are provided, together with expression systems for recombinant production of the lymphocyte homing receptor useful in therapeutic or diagnostic compositions.
Inventors
- 👤 Laurence A. Lasky
- 👤 Steven D. Rosen
- 👤 Scott E. Stachel
- 👤 Mark S. Singer
- 👤 Ted A. Yednock
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 10:12
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/32; C07K2319/33; C07K2319/70; C07K2319/705
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
46 words
A human LHR (HuLHR) comprising the carbohydrate binding domain, the epidermal growth factor domain and a complement binding domain of the amino acid sequence shown in FIG. 1 purified to show a single band on SDS-PAGE as visualized by silver stain, and having LHR biological activity.
Claim 2
Dependent Claim
46 words
A human LHR (HuLHR) comprising the carbohydrate binding domain, the epidermal growth factor domain and a complement binding domain of the amino acid sequence shown in FIG. 1 purified to show a single band on SDS-PAGE as visualized by silver stain, and having LHR biological activity.
Claim 3
Dependent Claim
14 words
The LHR of claim 1, wherein the LHR is not associated with native glycosylation.
Claim 4
Dependent Claim
11 words
The LHR of claim 1, wherein the LHR has variant glycosylation.
Claim 5
Dependent Claim
10 words
The LHR of claim 1 in a physiologically acceptable carrier.
Claim 6
Dependent Claim
13 words
The huLHR of claim 1 in which the transmembrane domain has been inactivated.
Claim 7
Dependent Claim
13 words
The huLHR of claim 1 from which the transmembrane domain has been deleted.
Claim 8
Dependent Claim
13 words
The huLHR of claim 6 from which the cytoplasmic domain has been deleted.
Claim 9
Dependent Claim
46 words
A murine LHR (MLHR) comprising the carbohydrate binding domain, the epidermal growth factor domain and a complement binding domain of the amino acid sequence shown in FIG. 2 purified to show a single band on SDS-PAGE as visualized by silver stain, and having LHR biological activity.
Claim 10
Dependent Claim
46 words
A murine LHR (MLHR) comprising the carbohydrate binding domain, the epidermal growth factor domain and a complement binding domain of the amino acid sequence shown in FIG. 2 purified to show a single band on SDS-PAGE as visualized by silver stain, and having LHR biological activity.
Claim 11
Dependent Claim
65 words
A polypeptide encoded by a DNA able to hybridize under stringent conditions to the complement of a DNA sequence encoding the carbohydrate binding domain, the epidermal growth factor domain or a complement binding domain of the LHR amino acid sequence shown in FIG. 1 or FIG. 2 purified to show a single band on SDS-PAGE as visualized by silver stain, and having LHR biological activity.
Claim 12
Dependent Claim
65 words
A polypeptide encoded by a DNA able to hybridize under stringent conditions to the complement of a DNA sequence encoding the carbohydrate binding domain, the epidermal growth factor domain or a complement binding domain of the LHR amino acid sequence shown in FIG. 1 or FIG. 2 purified to show a single band on SDS-PAGE as visualized by silver stain, and having LHR biological activity.
Claim 13
Dependent Claim
15 words
The LHR of claim 9 which binds to the high endothelial venules of lymphoid tissue.
Claim 14
Dependent Claim
48 words
The polypeptide of claim 9 wherein the stringent conditions are overnight incubation at 42° C. in a solution comprising: 20% formamide, 5XSSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5XDenhard's solution, 10% dextrane sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA.
Claim 15
Dependent Claim
39 words
The polypeptide of claim 9 encoded by a DNA able to hybridize under stringent conditions to the complement of a DNA encoding the carbohydrate binding domain of the LHR amino acid sequence shown in FIG. 1 or FIG. 2.
Claim 16
Dependent Claim
13 words
The polypeptide of claim 9 which is devoid of a functional transmembrane domain.
Claim 17
Dependent Claim
13 words
The polypeptide of claim 9 which is devoid of a functional cytoplasmic domain.
Claim 18
Dependent Claim
24 words
Mature HuLHR having the amino acid sequence shown in FIG. 1 purified to show a single band on SDS-PAGE as visualized by silver stain.
Claim 19
Dependent Claim
24 words
Mature HuLHR having the amino acid sequence shown in FIG. 1 purified to show a single band on SDS-PAGE as visualized by silver stain.
Claim 20
Dependent Claim
24 words
Mature MLHR having the amino acid sequence shown in FIG. 2 purified to show a single band on SDS-PAGE as visualized by silver stain.
Claim 21
Dependent Claim
24 words
Mature MLHR having the amino acid sequence shown in FIG. 2 purified to show a single band on SDS-PAGE as visualized by silver stain.
References Cited (Backward Citations)
US5002873A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-05-31 | 1996-11-30 | 1997-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-05-31 | 2000-11-30 | 2001-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-05-31 | 2004-11-30 | 2005-06-01 | $7700.00 | Window Closed / Lapsed |