Patent US5779688A successfully refreshed from Google Patents.
US5208166A
Expired / Lapsed
US
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Reactive chitosan coated articles and test kit for immunoassay
Assignee: Individual
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Filing: 1991-02-28
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Granted: 1993-05-04
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Expires: 2011-02-28
Abstract
Abstract A reagent coating material has been developed to modify the surface of articles used in assay procedures. Polymers of chitosan solubilized with an organic acid form into thin films when contacted to glass, plastic, metal or cellulose articles. The coated article may be used directly for immobilizing agents for assay, or may be further modified to increase the range of usefulness. The reagent coating solution itself may also be reacted to produce an activated reagent with surface coating properties. The invention embodies a kit of use in immunoassay procedures.
Inventors
- 👤 Mary S. Saunders
- 👤 Randall K. Pegg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/809
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
75 words
An apparatus for performing an immunoassay comprising: a) a substrate; b) a surface coating member on said substrate comprising a composition of chitosan, either natural or synthetic, and a polyvalent organic acid; c) said chitosan of said surface coating member further reacted with an oxidizer to produce a reactive moiety on said surface member; d) an immunochemical reagent member covalently bound to said surface coating member via said reactive moiety, thereby insolubilized on said substrate.
Claim 2
Independent Claim
75 words
An apparatus for performing an immunoassay comprising: a) a substrate; b) a surface coating member on said substrate comprising a composition of chitosan, either natural or synthetic, and a polyvalent organic acid; c) said chitosan of said surface coating member further reacted with an oxidizer to produce a reactive moiety on said surface member; d) an immunochemical reagent member covalently bound to said surface coating member via said reactive moiety, thereby insolubilized on said substrate.
Claim 3
Dependent Claim
12 words
The apparatus of claim 1 wherein said substrate comprises a microtiter well.
Claim 4
Dependent Claim
33 words
The apparatus according to claim 1 wherein said polyvalent organic acid is selected from the group consisting of citric acid, aspartic acid, malic acid, ascorbic acid, fumaric acid, glutaric acid, and combinations thereof.
Claim 5
Dependent Claim
52 words
The apparatus according to claim 1 wherein said immunochemical reagent member comprises antibodies which specifically bind an analyte selected from the group consisting of drugs of abuse, therapeutic drugs, hormones, antibiotics, mycotoxins, xenobiotics, microbial antigens, mammalian antigens, and tumor markers, said reagent member bound to said substrate by said surface coating member.
Claim 6
Dependent Claim
21 words
The apparatus of claim 1 wherein said oxidizer is selected from the group consisting of nitrite, hypochlorite, sulfite, and combinations thereof.
Claim 7
Dependent Claim
93 words
A kit for performing an immunoassay comprising a) a substrate, b) a composition for coating said substrate comprising chitosan and a polyvalent organic acid selected from the group consisting of citric acid, aspartic acid, malic acid, ascorbic acid, fumaric acid, glutaric acid, and combinations thereof in a single container; c) an oxidizing agent for reaction with the chitosan of said composition selected from the group consisting of nitrite, hypochlorite, sulfite, and combinations thereof in a separate container; and d) an agent for inhibiting nonspecific binding reactions on said substrate coated with said composition.
Claim 8
Dependent Claim
93 words
A kit for performing an immunoassay comprising a) a substrate, b) a composition for coating said substrate comprising chitosan and a polyvalent organic acid selected from the group consisting of citric acid, aspartic acid, malic acid, ascorbic acid, fumaric acid, glutaric acid, and combinations thereof in a single container; c) an oxidizing agent for reaction with the chitosan of said composition selected from the group consisting of nitrite, hypochlorite, sulfite, and combinations thereof in a separate container; and d) an agent for inhibiting nonspecific binding reactions on said substrate coated with said composition.
Claim 9
Independent Claim
45 words
An apparatus providing reactive surfaces useful in immunoassay procedures comprising: a) a substrate; b) a surface coating member comprised of a solution of chitosan and a polyvalent organic acid, said chitosan is covalently bound to a drug, drug hapten or xenobiotic useful in diagnostic screening.
Claim 10
Independent Claim
45 words
An apparatus providing reactive surfaces useful in immunoassay procedures comprising: a) a substrate; b) a surface coating member comprised of a solution of chitosan and a polyvalent organic acid, said chitosan is covalently bound to a drug, drug hapten or xenobiotic useful in diagnostic screening.
Claim 11
Dependent Claim
12 words
The apparatus of claim 7 wherein said substrate comprises a microtiter well.
Claim 12
Dependent Claim
32 words
The apparatus of claim 7 wherein said polyvalent organic acid is selected from the group consisting of citric acid, aspartic acid, malic acid, ascorbic acid, fumaric acid, glutaric acid, and combinations thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-05-03 | 1996-11-02 | 1997-05-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-05-03 | 2000-11-02 | 2001-05-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-05-03 | 2004-11-02 | 2005-05-04 | $7700.00 | Window Closed / Lapsed |