US5204455A
Expired / Lapsed
US
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Monomethoxytrityl protected oligonucleotides bound to a solid support
Assignee: Ionis Pharmaceuticals Inc
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Filing: 1992-02-10
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Granted: 1993-04-20
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Expires: 2012-02-10
Abstract
Abstract The present invention describes methods for the production of oligonucleotides under conditions which exploit the desirable characteristics, such as the property of sustaining high degrees of substitution, of functionalized organic polymeric supports while avoiding the sluggish kinetics and low rates of conversion which normally plague syntheses involving such solid supports. By employing the methods and materials disclosed, functionalized support, substituted to a degree of about 250 μmol/g, can be utilized at greater than 98% conversion levels for each sequential nucleotide coupling cycle, to provide unprecedented amounts of isolated oligonucleotide per gram of solid support.
Inventors
- 👤 Brian C. Froehler
- 👤 Kenneth M. Kent
- 👤 Sylvia Wu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 04:07
CPC Classifications
C; C07; C07H; C07H21/00; B; B01; B01J; B01J19/00; B01J19/0046; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H19/16; C07H19/20; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00281; B01J2219/00286; B01J2219/00497; B01J2219/00583; B01J2219/0059; B01J2219/00596; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
43 words
An oligonucleotide H-phosphonate, each internucleotide linkage of which contains an H-phosphonate moiety, which olionucleotide has a 5'-hydroxyl end and a 3'-hydroxyl end, one of which ends is bound to a solid support while the remaining end is blocked by a monomethoxytrityl protecting group.
Claim 2
Dependent Claim
43 words
An oligonucleotide H-phosphonate, each internucleotide linkage of which contains an H-phosphonate moiety, which olionucleotide has a 5'-hydroxyl end and a 3'-hydroxyl end, one of which ends is bound to a solid support while the remaining end is blocked by a monomethoxytrityl protecting group.
Claim 3
Dependent Claim
28 words
The oligonucleotide of claim 1 in which the 3'-hydroxyl end is bound to said solid support while the 5'-hydroxyl end is blocked by said monomethoxytrityl protecting the group.
Claim 4
Dependent Claim
28 words
The oligonucleotide of claim 1 in which the 5'-hydroxyl end is bound to said solid support while the 3'-hydroxyl end is blocked by said monomethoxytrityl protecting the group.
Claim 5
Dependent Claim
16 words
The oligonucleotide of claim 1 in which said solid support comprises a swellable polymeric organic support.
Claim 6
Dependent Claim
19 words
The oligonucleotide of claim 4 in which said swellable polymeric organic support is a polymer or copolymer of styrene.
Claim 7
Dependent Claim
25 words
The oligonucleotide of claim 4 in which said swellable polymeric organic solid support is selected form the group consisting of poly(styrene-co-divinylbenzene), poly(styrene-co-butadiene), poly(dimethylacrylamide-co-ethylenebisacrylamide), and poly(acryloylmorpholide).
Claim 8
Dependent Claim
27 words
The oligonucleotide of claim 5 in which said polymer or copolymer of styrene includes reactive functional groups selected from the group consisting of aminomethyl, hydroxymethyl, or carboxymethyl.
Claim 9
Dependent Claim
17 words
The oligonucleotide of claim 4 in which said swellable polymeric organic solid support is an aminomethylated poly(styrene-co-divinylbenzene).
Claim 10
Dependent Claim
14 words
The oligonucleotide of claim 1 which comprises from 2 to about 30 base monomers.
Claim 11
Dependent Claim
12 words
The oligonucleotide of claim 1 which comprises 30 or more base monomers.
References Cited (Backward Citations)
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| 3.5 Years (Year 4) | 1996-04-19 | 1996-10-19 | 1997-04-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-04-19 | 2000-10-19 | 2001-04-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-04-19 | 2004-10-19 | 2005-04-20 | $7700.00 | Window Closed / Lapsed |