Patent US5202007A successfully refreshed from Google Patents.
US5202007A
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Hydrophilic synthetic gels and their use in electrophoresis
Assignee: Elchrom Scientific AG
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Filing: 1991-05-07
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Granted: 1993-04-13
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Expires: 2011-05-07
Abstract
Abstract An electrophoretic method of resolving mixtures of molecules by putting the molecules in an ionic form; contracting them with a porous gel made up of a polymer comprising units derived from a monomer of the formula: ##STR1## wherein R 1 is H, CH 2 OH or (CHOH) m CH 2 OH, m being 1 or 2; R 2 is H, a monohydroxyalkyl radical, a polyhydroxyalkyl radical or a hydrocarbon radical; and n is an integer of 1-4; imposing electrophoretic conditions on said gel; migrating the different molecules different distances in said gel; and thereby separating said different molecules.
Inventors
- 👤 Branko Kozulic
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:41
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; C; C08; C08F; C08F20/00; C08F20/02; C08F20/52; C08F20/54; C08F20/58
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
89 words
A method of separating molecules which comprises subjecting such molecules to electrophoresis while in effective contact with a separation medium comprising a polymer comprising a monomer of the formula: ##STR4## wherein R 1 is H, CH 2 OH or (CHOH) m CH 2 OH, m being 1 or 2; R 2 is hydrogen, monohydroxyalkyl, polyhydroxyalkyl, or hydrocarbon radical; R 3 is H or CH 3 ; and n is an integer of 1-4; migrating ionic species of said molecules in said medium thereby separating said species from each other.
Claim 2
Independent Claim
89 words
A method of separating molecules which comprises subjecting such molecules to electrophoresis while in effective contact with a separation medium comprising a polymer comprising a monomer of the formula: ##STR4## wherein R 1 is H, CH 2 OH or (CHOH) m CH 2 OH, m being 1 or 2; R 2 is hydrogen, monohydroxyalkyl, polyhydroxyalkyl, or hydrocarbon radical; R 3 is H or CH 3 ; and n is an integer of 1-4; migrating ionic species of said molecules in said medium thereby separating said species from each other.
Claim 3
Dependent Claim
78 words
A method as claimed in claim 1, wherein said separation medium comprises an aqueous cross-linked gel comprising a polymer comprising a monomer of the formula: ##STR5## wherein R 1 is H, CH 2 OH or (CHOH) m CH 2 OH, m being 1 or 2; R 2 is H or CH 3 ; R 3 is H or CH 3 ; and n is an integer of 1-4; and a cross-linker with at least two polymerizable double bonds.
Claim 4
Dependent Claim
22 words
A method of claim 2, wherein the said gel is polymerized in a mold and used in that mold for electrophoretic separation.
Claim 5
Dependent Claim
14 words
A method of claim 3, wherein the gel is in form of a rod.
Claim 6
Dependent Claim
14 words
A method of claim 3, wherein the gel is in form of a sheet.
Claim 7
Dependent Claim
15 words
A method of claim 3, wherein the gel is in form of a thin filament.
Claim 8
Dependent Claim
20 words
A method of claim 2, wherein the said gel is in form of a sheet grafted onto a solid support.
Claim 9
Dependent Claim
13 words
A method of claim 7, wherein the solid support is a plastic material.
Claim 10
Dependent Claim
11 words
A method of claim 7, wherein the solid support is glass.
Claim 11
Independent Claim
15 words
A method of one of claims 1 or 2, wherein the ionic species comprise polypeptides.
Claim 12
Independent Claim
15 words
A method of one of claims 1 or 2, wherein the ionic species comprise polynucleotides.
Claim 13
Independent Claim
18 words
A method of one of claims 1 or 2, wherein the ionic species have molecular weight below 1000.
Claim 14
Independent Claim
20 words
A method as claimed in one of claims 1 or 2 including the further step of detecting said separated species.
Claim 15
Independent Claim
21 words
A method of one of claims 1 or 2, including detection of said separated species by interaction with a specific stain.
Claim 16
Independent Claim
23 words
A method of one of claims 1 or 2, including transferring said separated species to another material and thence detecting said separating species.
Claim 17
Independent Claim
29 words
A method of one of claims 1 or 2, including providing a detector fixed in relation to said gel, and detecting said separated species by interaction with said detector.
Claim 18
Independent Claim
20 words
A method of one of claims 1 or 2, including detecting said separated species by interaction with a scanning detector.
Claim 19
Dependent Claim
15 words
A method as claimed in claim 1 wherein said R 2 contains 1-30 carbon atoms.
References Cited (Backward Citations)
US4925545A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1996-04-12 | 1996-10-12 | 1997-04-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-04-12 | 2000-10-12 | 2001-04-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-04-12 | 2004-10-12 | 2005-04-13 | $7700.00 | Window Closed / Lapsed |