Patent US5200270A successfully refreshed from Google Patents.
US5200270A
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Carrier for a biologically active component for immunoassay or enzymatic reaction
Assignee: Tosoh Corp
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Filing: 1991-09-19
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Granted: 1993-04-06
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Expires: 2011-09-19
Abstract
Abstract A carrier for a biologically active component for immunoassay or enzymatic reaction, which comprises: a) a thermoplastic resin bead having an average diameter of from 0.05 to 20 mm, b) from 1 to 25% by weight, based on the bead, of a magnetically responsive powder bonded to the bead, and c) a polymer coated thereon in a thickness of from 2 to 30 mu m, said polymer having a number average molecular weight of from 200 to 10,000 and having functional groups capable of binding, or being activated to bind, the biologically active component.
Inventors
- 👤 Hiroshi Ishida
- 👤 Yuji Higo
- 👤 Masuo Inoue
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 18:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54313; G01N33/54326; G01N33/5434; G01N2446/00; G01N2446/10; G01N2446/80; G01N2446/84; G01N2446/90; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2998
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
104 words
A carrier for a biologically active component for immunoassay or enzymatic reaction, which comprises: a) a thermoplastic resin bead having an average diameter of from 0.05 to 20 mm, b) from 1 to 25% by weight, based on the bead, of a magnetically responsive powder having an average particle size of from 0.01 to 10 μm bound on the bead, and c) a polymer coated thereon in a thickness of from 2 to 30 μm, said polymer having a number average degree of polymerization of from 20 to 5000 and having or capable of having functional groups capable of binding the biologically active component.
Claim 2
Dependent Claim
104 words
A carrier for a biologically active component for immunoassay or enzymatic reaction, which comprises: a) a thermoplastic resin bead having an average diameter of from 0.05 to 20 mm, b) from 1 to 25% by weight, based on the bead, of a magnetically responsive powder having an average particle size of from 0.01 to 10 μm bound on the bead, and c) a polymer coated thereon in a thickness of from 2 to 30 μm, said polymer having a number average degree of polymerization of from 20 to 5000 and having or capable of having functional groups capable of binding the biologically active component.
Claim 3
Dependent Claim
28 words
The carrier according to claim 1, wherein the functional groups of the polymer (c) are selected from the group consisting of ##STR8## --CHO, --OH and --NH 2 .
Claim 4
Dependent Claim
37 words
The carrier according to claim 1, wherein the bead is made of a thermoplastic resin selected from the group consisting of a polyolefin resin, a vinyl resin, a polyester resin, a polyamide resin, and a mixture thereof.
Claim 5
Dependent Claim
12 words
The carrier according to claim 1, wherein the bead is substantially spherical.
Claim 6
Dependent Claim
56 words
The carrier according to claim 1, wherein the magnetically responsive powder has an average particle size of from 0.01 to 10 μm and is made of iron, tri-iron tetroxide, nickel, iron-cobalt, silicon steel or a soft ferrite of the formula MFe 2 O 4 wherein M is Mn, Zn, Ni, Cd, Cu, Mg, Sr or Ba.
Claim 7
Dependent Claim
52 words
The carrier according to claim 1, wherein the polymer (c) is a polymer of a monomer having the formula RCH═CR'--CHO wherein each of R and R' is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group or a phenyl group, and having a total of from 3 to 10 carbon atoms.
Claim 8
Dependent Claim
57 words
The carrier according to claim 1, wherein the polymer (c) is a polymer having a repeating unit of the formula: ##STR9## wherein each of R and R' is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group or a phenyl group, and obtained from a monomer having a total of from 6 to 12 carbon atoms.
Claim 9
Dependent Claim
65 words
The carrier according to claim 1, wherein the polymer (c) is a polymer having --OH groups obtained by hydrolyzing a polymer having a repeating unit of the formula: ##STR10## wherein each of R and R' is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group or a phenyl group, and obtained from a monomer having a total of from 6 to 12 carbon atoms.
Claim 10
Dependent Claim
100 words
The carrier according to claim 1, wherein the polymer (c) is a polymer having --NH 2 groups obtained by aminating a polymer having a repeating unit of the formula: ##STR11## wherein each of R and R' is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group or a phenyl group, and obtained from a monomer having a total of from 6 to 12 carbon atoms, with a diamine having the formula NH 2 --R"--NH 2 wherein R" is an alkyl group, a halogen-substituted alkyl group or a phenyl group having a total of from 2 to 12 carbon atoms.
Claim 11
Dependent Claim
26 words
The carrier according to claim 1, wherein the polymer (c) is polyglycidyl methacrylate, a glycidyl methacrylate-tetraethyleneglycol dimethacrylate copolymer, an acrolein-tetraethyleneglycol dimethacrylate copolymer, polystyrene or polyvinyl chloride.
Claim 12
Dependent Claim
31 words
The carrier according to claim 1, wherein the functional groups of the polymer (c) are --OH groups activated by treatment with carbodiimidazole or --NH 2 groups activated by treatment with glutaraldehyde.
Claim 13
Dependent Claim
27 words
The carrier according to claim 1, wherein a biologically active component selected from the group consisting of antigens, antibodies and enzymes, is bound to the polymer (c).
Claim 14
Dependent Claim
59 words
A process for preparing the carrier defined by claim 1, which comprises depositing a magnetically responsive powder on the surface of the thermoplastic resin bead in an amount of from 1 to 25% by weight, based on the bead, and forming a layer of the polymer (c) on the bead in a thickness of from 2 to 30 μm.
Claim 15
Dependent Claim
41 words
The process according to claim 13, wherein the layer of the polymer (c) is formed by polymerizing a monomer having functional groups selected from the group consisting of ##STR12## --CHO or --OH to form a polymer having the corresponding functional groups.
Claim 16
Dependent Claim
30 words
The process according to claim 14, wherein the polymer having ##STR13## is hydrolyzed to convert the groups to --OH groups or treated with a diamine to introduce --NH 2 groups.
Claim 17
Dependent Claim
18 words
The process according to claim 14, wherein the polymer having --OH groups is treated with N,N'-carbonyldiimidazole for activation.
Claim 18
Dependent Claim
25 words
The process according to claim 14, wherein the polymer having ##STR14## is partially hydrolyzed and reacted with a silane coupling agent having --NH 2 groups.
Claim 19
Dependent Claim
19 words
The process according to claim 15, wherein the polymer having --NH 2 groups is treated with glutaraldehyde for activation.
Claim 20
Dependent Claim
76 words
The process according to claim 13, wherein the thermoplastic bead is substantially spherical and is prepared by dispersing particles of a thermoplastic resin having a substantially uniform shape in a medium in which the resin is insoluble, at a temperature lower than the melting point of the resin and then heating the dispersion at a temperature of from the melting point of the resin to a temperature not higher than 30° C. above the melting point.
Claim 21
Dependent Claim
20 words
The process according to claim 13, which includes an additional step of treating the carrier with the biologically active component.
Claim 22
Dependent Claim
23 words
The carrier according to claim 1, wherein the bead is made of a thermoplastic resin wherein said thermoplastic resin is a copolymer resin.
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