US5198353A
Expired / Lapsed
US
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Method for preparing stabilized enzyme dispersion
Assignee: Novozymes AS
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Filing: 1989-07-11
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Granted: 1993-03-30
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Expires: 2009-07-11
Abstract
Abstract A convenient method for preparing a stabilized aqueous enzyme dispersion comprises: (1) precipitating a water-soluble polymer from aqueous solution to form an aqueous dispersion, and (2) before, during or after (1), contacting the dissolved or dispersed polymer with an aqueous solution or fine aqueous dispersion of enzyme. Using this method, substantial improvement of the enzyme stability during storage can be obtained with surprisingly little polymer (relative to enzyme). And enzyme stabilization can, surprisingly, even be obtained by contacting precipitated polymer with dissolved enzyme. The stabilizing effect therefore appears not to be due (or at least not primarily due) to encapsulation.
Inventors
- 👤 John Hawkins
- 👤 Philip Chadwick
- 👤 Edward T. Messenger
- 👤 Mads Lykke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 09:06
CPC Classifications
C; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38663
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
62 words
A method for preparing a stabilized enzyme dispersion, comprising: (1) precipitating a water-soluble polymer from a single phase, aqueous solution to form an aqueous dispersion, and (2) before, simultaneously with or after precipitating the polymer, contacting the dissolved or dispersed polymer with an aqueous solution or fine aqueous dispersion of an enzyme without any covalent bonding between the polymer and the enzyme.
Claim 2
Independent Claim
62 words
A method for preparing a stabilized enzyme dispersion, comprising: (1) precipitating a water-soluble polymer from a single phase, aqueous solution to form an aqueous dispersion, and (2) before, simultaneously with or after precipitating the polymer, contacting the dissolved or dispersed polymer with an aqueous solution or fine aqueous dispersion of an enzyme without any covalent bonding between the polymer and the enzyme.
Claim 3
Dependent Claim
22 words
The method according to claim 1, wherein said enzyme is selected from the group consisting of a protease, amylase, cellulase and lipase.
Claim 4
Dependent Claim
32 words
The method according to claim 1, wherein said polymer is selected from the group consisting of polyvinyl alcohol, polyvinyl pyrrolidone, poly-C 1-4 carboxylic acid salt, carboxymethyl cellulose salt, gelatin and guar gum.
Claim 5
Dependent Claim
31 words
The method according to claim 1, wherein said polymer is a partially hydrolyzed polyvinyl ester of a C 1-4 carboxylic acid having a degree of hydrolysis of from 25 to 90%.
Claim 6
Dependent Claim
23 words
The method according to claim 3, wherein said polyvinyl pyrrolidone has an average molecular weight in the range of about 1,000 to 1,500,000.
Claim 7
Dependent Claim
24 words
The method according to claim 1, wherein the weight ratio of said polymer to said enzyme is in the range of 0.03 to 5.
Claim 8
Dependent Claim
20 words
The method according to claim 1, wherein the polymer is precipitated by contacting with an effective amount of a precipitant.
Claim 9
Dependent Claim
16 words
The method according to claim 7, wherein the precipitant is an electrolyte or an organic solvent.
Claim 10
Dependent Claim
27 words
The method according to claim 8, wherein said electrolyte is selected from the group consisting of sodium sulphate, sodium citrate, sodium tripolyphosphate, sodium carbonate and ammonium sulphate.
Claim 11
Dependent Claim
14 words
The method according to claim 8, wherein said organic solvent is acetone or ethanol.
Claim 12
Dependent Claim
13 words
The method according to claim 1, wherein the polymer is precipitated by evaporation.
Claim 13
Dependent Claim
18 words
The method according to claim 1, wherein precipitation of said enzyme occurs simultaneously with precipitation of said polymer.
Claim 14
Dependent Claim
26 words
The method according to claim 12, wherein a solution containing said polymer and said enzyme is contacted with a precipitant to directly form an enzyme dispersion.
Claim 15
Dependent Claim
20 words
The method according to claim 12, wherein a finely divided coprecipitate of the enzyme and polymer is dispersed in water.
Claim 16
Dependent Claim
17 words
The method according to claim 1, wherein the precipitated, dispersed polymer is contacted with the dissolved enzyme.
Claim 17
Dependent Claim
18 words
The method according to claim 1, wherein the dissolved polymer is contacted with the finely divided solid enzyme.
Claim 18
Dependent Claim
38 words
The method according to claim 12, in which a clear solution comprising polyvinyl pyrrolidone and an enzyme selected from the group consisting of a protease, an amylase, a cellulase and a lipase if employed as the single-phase solution.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-03-29 | 1996-09-28 | 1997-03-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-03-29 | 2000-09-28 | 2001-03-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-03-29 | 2004-09-28 | 2005-03-30 | $7700.00 | Window Closed / Lapsed |