Patent US5198345A successfully refreshed from Google Patents.
US5198345A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Vectors in use in filamentous fungi
Assignee: DSM IP Assets BV
•
Filing: 1985-12-20
•
Granted: 1993-03-30
•
Expires: 2005-12-20
Abstract
Abstract Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as Aspergillus may be provided with foreign or natural coding regions associated with foreign or natural promoter sequences and optionally signal peptide sequences which can be used to control the expression and/or secretion of the proteins encoded by these coding regions.
Inventors
- 👤 David I. Gwynne
- 👤 Francis P. Buxton
- 👤 Mark H. Pickett
- 👤 Roger W. Davies
- 👤 Claudio Scazzocchio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 01:17
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C12N9/2414; C12N9/2422; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/56; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N9/0004; C12N9/0006; C12N9/0008; C12N9/2428; C12N9/2434; C12Y; C12Y101/00; C12Y101/01; C12Y101/01001; C12Y102/00; C12Y102/01; C12Y102/01003; C12Y302/00; C12Y302/01; C12Y302/01003; C12Y302/01004; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
55 words
A DNA construct for use in transforming an Aspergillus host to obtain expression therein of a polypeptide foreign thereto, said DNA construct comprising promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus host.
Claim 2
Dependent Claim
55 words
A DNA construct for use in transforming an Aspergillus host to obtain expression therein of a polypeptide foreign thereto, said DNA construct comprising promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus host.
Claim 3
Dependent Claim
14 words
A DNA construct according to claim 1, wherein said Aspergillus host is Aspergillus niger.
Claim 4
Dependent Claim
14 words
A DNA construct according to claim 1, wherein said Aspergillus host is Aspergillus nidulans.
Claim 5
Dependent Claim
71 words
A DNA construct for use in transforming an Aspergillus nidulans host to obtain expression therein of a polypeptide foreign thereto, said DNA construct comprising promoter DNA corresponding in sequence to the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus nidulans host.
Claim 6
Dependent Claim
71 words
A DNA construct for use in transforming an Aspergillus nidulans host to obtain expression therein of a polypeptide foreign thereto, said DNA construct comprising promoter DNA corresponding in sequence to the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus nidulans host.
Claim 7
Dependent Claim
27 words
A DNA construct according to claim 4, wherein said promoter DNA corresponds in sequence to the promoter region of the alcohol dehydrogenase 1 gene of Aspergillus nidulans.
Claim 8
Dependent Claim
26 words
A DNA construct according to claim 4, wherein said promoter DNA corresponds in sequence to the promoter region of the aldehyde dehydrogenase gene of Aspergillus nidulans.
Claim 9
Dependent Claim
50 words
An Aspergillus strain which upon culturing expresses a polypeptide foreign thereto as a result of having been transformed by a DNA construct in which DNA coding for the foreign polypeptide is linked operably to promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger.
Claim 10
Dependent Claim
50 words
An Aspergillus strain which upon culturing expresses a polypeptide foreign thereto as a result of having been transformed by a DNA construct in which DNA coding for the foreign polypeptide is linked operably to promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger.
Claim 11
Dependent Claim
25 words
A strain according to claim 7, wherein said strain is a transformed Aspergillus species selected from the group consisting of Aspergillus nidulans and Aspergillus niger.
Claim 12
Dependent Claim
12 words
A strain according to claim 8, which is an Aspergillus niger transformant.
Claim 13
Dependent Claim
65 words
An Aspergillus nidulans strain which upon culturing expresses a polypeptide foreign thereto as a result of having been transformed by a DNA construct in which DNA coding for the foreign polypeptide is linked operably to promoter DNA corresponding in sequence to the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene.
Claim 14
Dependent Claim
65 words
An Aspergillus nidulans strain which upon culturing expresses a polypeptide foreign thereto as a result of having been transformed by a DNA construct in which DNA coding for the foreign polypeptide is linked operably to promoter DNA corresponding in sequence to the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene.
Claim 15
Dependent Claim
28 words
An Aspergillus nidulans strain according to claim 10, wherein said promoter DNA corresponds in sequence to the promoter region of the alcohol dehydrogenase 1 gene of Aspergillus nidulans.
Claim 16
Independent Claim
53 words
A method for producing a polypeptide, said method comprising the step of culturing an Aspergillus strain transformed by a DNA construct in which DNA coding for a polypeptide foreign to said Aspergillus strain is linked operably to promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger.
Claim 17
Independent Claim
53 words
A method for producing a polypeptide, said method comprising the step of culturing an Aspergillus strain transformed by a DNA construct in which DNA coding for a polypeptide foreign to said Aspergillus strain is linked operably to promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger.
Claim 18
Dependent Claim
26 words
A method according to claim 12, wherein the cultured strain is a transformed Aspergillus species selected from the group consisting of Aspergillus nidulans and Aspergillus niger.
Claim 19
Dependent Claim
15 words
A method according to claim 13, wherein said cultured strain in an Aspergillus niger transformant.
Claim 20
Independent Claim
68 words
A method for producing a polypeptide, said method comprising the step of culturing an Aspergillus nidulans strain transformed by a DNA construct in which DNA coding for a polypeptide foreign to said Aspergillus nidulans is linked operably to promoter DNA corresponding in sequence with the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene.
Claim 21
Independent Claim
68 words
A method for producing a polypeptide, said method comprising the step of culturing an Aspergillus nidulans strain transformed by a DNA construct in which DNA coding for a polypeptide foreign to said Aspergillus nidulans is linked operably to promoter DNA corresponding in sequence with the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene.
Claim 22
Dependent Claim
26 words
A method according to claim 15, wherein said promoter DNA corresponds in sequence to the promoter region of the alcohol dehydrogenase 1 gene of Aspergillus nidulans.
Claim 23
Dependent Claim
21 words
A method according to claim 16, wherein said strain is cultured in a culturing medium containing an expression-inducing amount of threonine.
References Cited (Backward Citations)
EP0099226A1
Cited
WO1984002921A2
Cited
EP0127304A1
Cited
EP0136007A2
Cited
EP0173378B1
Cited
EP0181213A2
Cited
EP0215594B1
Cited
EP0225078A2
Cited
WO1987004464A1
Cited
EP0238023B1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-03-29 | 1996-09-28 | 1997-03-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-03-29 | 2000-09-28 | 2001-03-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-03-29 | 2004-09-28 | 2005-03-30 | $7700.00 | Window Closed / Lapsed |