US5175082A
Expired / Lapsed
US
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Method of characterizing genomic dna
Assignee: Imperial Chemical Industries Ltd
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Filing: 1987-03-19
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Granted: 1992-12-29
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Expires: 2007-03-19
Abstract
Abstract Test samples of genomic DNA may be characterized by the use of polynucleotide probes each of which is specific for an informative genetic locus. Such probes may be prepared by the use of probes which are capable of differentiating DNA by reference to more than one polymorphic minisatellite region or hypervariable locus. The polynucleotides and probes of the invention are of use for genetic identification purposes, paternity and maternity testing and particularly in forensic medicine.
Inventors
- 👤 Alec J. Jeffreys
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 10:54
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6827; C12Q1/6876; C12Q2600/00; C12Q2600/156; C12Q2600/172
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
137 words
A method of characterizing a test sample of genomic DNA from a donor by reference to one or more controls which method comprises: i) fragmenting said sample DNA with at least one restriction enzyme which does not cleave a sequence corresponding to a tandem repeat, ii) probing the DNA fragments with a polynucleotide probe which specifically hybridizes to a single minisatellite region (to the exclusion of other regions), wherein said probe consists essentially of up to 10,000 tandem repeats of a repeat sequence unit being one of ##STR7## wherein Y is C, T or U, X is G or C, R is A or G and T is T or U, or a sequence complementary thereto of identical length, iii) detecting hybridized fragments of DNA, and iv) comparing the hybridized fragments with the said control or controls.
Claim 2
Independent Claim
137 words
A method of characterizing a test sample of genomic DNA from a donor by reference to one or more controls which method comprises: i) fragmenting said sample DNA with at least one restriction enzyme which does not cleave a sequence corresponding to a tandem repeat, ii) probing the DNA fragments with a polynucleotide probe which specifically hybridizes to a single minisatellite region (to the exclusion of other regions), wherein said probe consists essentially of up to 10,000 tandem repeats of a repeat sequence unit being one of ##STR7## wherein Y is C, T or U, X is G or C, R is A or G and T is T or U, or a sequence complementary thereto of identical length, iii) detecting hybridized fragments of DNA, and iv) comparing the hybridized fragments with the said control or controls.
Claim 3
Dependent Claim
49 words
The method according to claim 1 wherein said method establishes the identity of the test sample donor with one or more control sample donors, wherein the control sample(s) are fragmented in an identical manner and a comparison is made of the hybridized fragments from the control and test samples.
Claim 4
Dependent Claim
50 words
The method according to claim 1 wherein said method establishes a family connection between the test sample donor and one or more control sample donors, wherein the control sample(s) are fragmented in an identical manner and a comparison is made of the hybridized fragments from the control and test samples.
Claim 5
Dependent Claim
38 words
The method as claimed in claim 1 wherein said probing is effected using at least two of said polynucleotide probes either in a sequence of tests or in a single test using a mixture of said polynucleotide probes.
Claim 6
Dependent Claim
22 words
The method as claimed in claim 1 wherein the repeat unit sequence of the polynucleotide probe consists of one of sequences 3-9.
Claim 7
Dependent Claim
18 words
The method as claimed in claim 1 wherein the polynucleotide probe is from 9 bp to 45 bp.
Claim 8
Dependent Claim
17 words
The method as claimed in claim 1 wherein the polynucleotide probe is wholly in single stranded form.
Claim 9
Dependent Claim
26 words
A method as claimed in claim 1 wherein the polynucleotide probe is selected from the group consisting of pλg3, λMS1, λMS8(1), λMS8(2), λMS31, λMS32 and λMS43.
References Cited (Backward Citations)
EP0084796A2
Cited
WO1983003260A1
Cited
GB2135774A
Cited
EP0164054A1
Cited
GB2166445A
Cited
WO1986002948A1
Cited
WO1986006102A1
Cited
WO1986007464A1
Cited
WO1989007658A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-29 | 1996-06-29 | 1996-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-29 | 2000-06-29 | 2000-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-29 | 2004-06-29 | 2004-12-29 | $7700.00 | Window Closed / Lapsed |