EP0084796A2
Expired / Lapsed
EP
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HLA typing method and cDNA probes used therein
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1983-01-11
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Granted: —
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Expires: 2003-01-11
Abstract
Abstract HLA typing based on restriction length polymorphism is carried out by: digesting an individual's HLA DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; subjecting the digest to genomic blotting using a labeled cDNA hybridization probe that is complementary to an HLA DNA sequence involved in the polymorphism; and comparing the resulting genomic blotting pattern with a standard This technique may be adapted to make paternity or transplant or transfusion compatibility determinations or to make disease association correlations to diagnose diseases or predict susceptibility to diseases. Locus specific cDNA hybridization probes, particularly probes for genes of Class II loci (D and OR loci), for use in the typing procedure are described.
Inventors
- 👤 Henry A. Erlich
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 20:19
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6881; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/70539; C12Q1/6813; C12Q1/6827; C12Q1/683; C12Q2600/00; C12Q2600/156; C12Q2600/172
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
105 words
An HLA typing method based on HLA DNA restriction fragment length polymorphism characterized by the following steps: (a) digesting HLA DNA from an individual with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; (b) subjecting the digest of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to an HLA DNA locus sequence involved in the polymorphism; and (c) comparing the genomic blotting or resolution pattern obtained in (b) with a standard genomic blotting or resolution pattern for said HLA DNA sequence obtained using said restriction endonuclease and an equivalent labeled cDNA probe.
Claim 2
Dependent Claim
105 words
An HLA typing method based on HLA DNA restriction fragment length polymorphism characterized by the following steps: (a) digesting HLA DNA from an individual with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; (b) subjecting the digest of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to an HLA DNA locus sequence involved in the polymorphism; and (c) comparing the genomic blotting or resolution pattern obtained in (b) with a standard genomic blotting or resolution pattern for said HLA DNA sequence obtained using said restriction endonuclease and an equivalent labeled cDNA probe.
Claim 3
Dependent Claim
37 words
The method of Claim 1 further characterized in that the probe is specific to a single HLA locus and the restriction endonuclease is one that produces a polymorphic digestion pattern associated with the DNA of said locus.
Claim 4
Dependent Claim
16 words
The method of Claim 2 further characterized in that said locus is a Class II locus.
Claim 5
Dependent Claim
23 words
The method of Claim 3 further characterized in that the locus is the DRa locus, a DRa related locus, or the DR8 locus.
Claim 6
Dependent Claim
60 words
The method of Claim 3 further characterized in that: (i) the locus is the DRa locus and the restriction endonuclease is BglII or EcoRV, or (ii) the locus is a DRa related locus and the restriction endonuclease is BglII, or (iii) the locus is the DR8 locus or a DRβ related locus and the restriction endonuclease is EcoRI or BglII.
Claim 7
Independent Claim
128 words
A method for determining the paternity of an individual based on HLA DNA restriction fragment length polymorphism characterized by the following steps: (a) digesting HLA DNA of the mother of the individual, the suspected father of the individual, and the individual with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; (b) subjecting each of the digests of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to an HLA DNA sequence involved in the polymorphism; and (c) comparing the genomic blotting or resolution patterns obtained in (b) to determine correspondence between the individual's pattern and the mother's pattern and suspected father's pattern and thereby determining whether the suspected father is the actual father of the individual.
Claim 8
Independent Claim
128 words
A method for determining the paternity of an individual based on HLA DNA restriction fragment length polymorphism characterized by the following steps: (a) digesting HLA DNA of the mother of the individual, the suspected father of the individual, and the individual with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; (b) subjecting each of the digests of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to an HLA DNA sequence involved in the polymorphism; and (c) comparing the genomic blotting or resolution patterns obtained in (b) to determine correspondence between the individual's pattern and the mother's pattern and suspected father's pattern and thereby determining whether the suspected father is the actual father of the individual.
Claim 9
Independent Claim
118 words
A method for determining transplant or transfusion compatibility based on HLA DNA restriction fragment length polymorphism characterized by the following steps: (a) digesting HLA DNA of the transplant or transfusion donor and the transplant or transfusion host with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; (b) subjecting each of the digests of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to an HLA DNA sequence involved in the polymorphism; and (c) comparing the genomic blotting or resolution patterns obtained in (b) to determine correspondence therebetween and thereby determining whether the transplant or transfusion donor's HLA is compatible with the transplant or transfusion host's HLA.
Claim 10
Independent Claim
118 words
A method for determining transplant or transfusion compatibility based on HLA DNA restriction fragment length polymorphism characterized by the following steps: (a) digesting HLA DNA of the transplant or transfusion donor and the transplant or transfusion host with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; (b) subjecting each of the digests of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to an HLA DNA sequence involved in the polymorphism; and (c) comparing the genomic blotting or resolution patterns obtained in (b) to determine correspondence therebetween and thereby determining whether the transplant or transfusion donor's HLA is compatible with the transplant or transfusion host's HLA.
Claim 11
Independent Claim
108 words
A method of determining whether an individual is susceptible to or has a disease characterized by the following steps: (a) digesting HLA DNA from the individual with a restriction endonuclease that produces a polymorphic digestion pattern of an HLA locus that is associated with the disease; (b) subjecting the digest of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to the DNA sequence of said HLA locus; (c) comparing the genomic blotting or resolution pattern obtained in (b) with a standard pattern of an individual having said disease obtained using said restriction endonuclease and an equivalent labeled cDNA probe.
Claim 12
Independent Claim
108 words
A method of determining whether an individual is susceptible to or has a disease characterized by the following steps: (a) digesting HLA DNA from the individual with a restriction endonuclease that produces a polymorphic digestion pattern of an HLA locus that is associated with the disease; (b) subjecting the digest of (a) to genomic blotting or solution hybridization - resolution using a labeled cDNA probe that is complementary to the DNA sequence of said HLA locus; (c) comparing the genomic blotting or resolution pattern obtained in (b) with a standard pattern of an individual having said disease obtained using said restriction endonuclease and an equivalent labeled cDNA probe.
Claim 13
Dependent Claim
13 words
The method of claim 8 wherein the disease is insulin dependent diabetes mellitus.
Claim 14
Dependent Claim
14 words
The method of claim 8 wherein said HLA locus is a Class II locus.
References Cited (Backward Citations)
No citation records indexed.
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