US5173401A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection of neisseria gonorrohoeae
Assignee: Roche Diagnostics GmbH
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Filing: 1990-07-12
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Granted: 1992-12-22
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Expires: 2010-07-12
Abstract
Abstract An oligonucleotide probe specific for Neisseria gonorrhoeae is selected from the oligonucleotides RS2* and RS3*, RS2** and RS3**. It can have further nucleotides at its 5' end and/or 3' end. This probe is suitable for the specific detection of nucleic acids of the pathogenic species N. gonorrhoeae by hybrid formation under hybridization conditions.
Inventors
- 👤 Karin Wolff
- 👤 Anne Stern
- 👤 Peter Buckel
- 👤 Erko Stackenbrandt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 10:51
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
54 words
A Neisseria gonorrhoeae-specific oligonucleotide probe, selected from the group consisting of a) an oligonucleotide RS2* having the sequence TACCAAGCAA TCAAGTTG, b) an olignoucleotide RS3* having the sequence CCACCCGAGA AGCAAGCTTC CCTGTGCT, c) an oligonucleotide RS2** having the sequence ACGCTACCAA GCAATCAAGT TGCCCAACAG CTAATTGACA T and/or d) an oligonucleotide RS3** having the sequence GCCACCCGAG AAGCAAGCTT CCCTGTGCTG CCGTCC.
Claim 2
Dependent Claim
54 words
A Neisseria gonorrhoeae-specific oligonucleotide probe, selected from the group consisting of a) an oligonucleotide RS2* having the sequence TACCAAGCAA TCAAGTTG, b) an olignoucleotide RS3* having the sequence CCACCCGAGA AGCAAGCTTC CCTGTGCT, c) an oligonucleotide RS2** having the sequence ACGCTACCAA GCAATCAAGT TGCCCAACAG CTAATTGACA T and/or d) an oligonucleotide RS3** having the sequence GCCACCCGAG AAGCAAGCTT CCCTGTGCTG CCGTCC.
Claim 3
Dependent Claim
40 words
The probe according to claim 1, having up to 10 further nucleotides at its 5' and/or 3' end, said nucleotides corresponding to those nucleotides which are present at these positions in the natural gene from which the probes are derived.
Claim 4
Dependent Claim
10 words
The probe according to claim 1, having a detectable label.
Claim 5
Dependent Claim
44 words
A new method for the detection of the pathogenic Neisseria species Neisseria gonorrheae comprising: a) contacting under hybridizing conditions a sample suspected of containing Neisseria gonorrheae and an oligonucleotide probe according to claim 1; and b) detecting hybrid formation if Neisseria gonorrheae is present.
References Cited (Backward Citations)
EP0237737A2
Cited
WO1988003957A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-22 | 1996-06-22 | 1996-12-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-22 | 2000-06-22 | 2000-12-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-22 | 2004-06-22 | 2004-12-22 | $7700.00 | Window Closed / Lapsed |