EP0237737A2
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EP
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A composition specific for Neisseria gonorrhoea
Assignee: Enzo Biochem Inc
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Filing: 1987-01-29
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Granted: —
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Expires: 2007-01-29
Abstract
Abstract A composition comprising discrete nucleotide sequences that are specific for Neissearia gonorrhoeae. The compositions can be utilized to detect Neisseria gonorrhoeae chromosomal DNA and thus, Neisseria gonorrhoeae.
Inventors
- 👤 Andrew Lo
- 👤 Huey-Lang Yang
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 06:21
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/22; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/10; C12Q1/68; C12Q1/6813; C12Q1/6876; C12Q1/6888; C12Q1/689; C12Q1/70; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
65 words
A composition of matter that is specific for Neisseria gonorrhoeae comprising at least one discrete nucleotide sequence wherein said composition gives a ratio of the lowest of the average amount of said composition hybridized to purified chromosomal DNA of the following six strains of Neisseria gonorrhoeae : l) ATCC 53420 2) ATCC 5342l 3) ATCC 53422 4) ATCC 53423 5) ATCC 53424 6) ATCC 53425,
Claim 2
Dependent Claim
65 words
A composition of matter that is specific for Neisseria gonorrhoeae comprising at least one discrete nucleotide sequence wherein said composition gives a ratio of the lowest of the average amount of said composition hybridized to purified chromosomal DNA of the following six strains of Neisseria gonorrhoeae : l) ATCC 53420 2) ATCC 5342l 3) ATCC 53422 4) ATCC 53423 5) ATCC 53424 6) ATCC 53425,
Claim 3
Dependent Claim
59 words
normalized to equal amounts of said purified chromosomal DNA of said six strains of Neisseria gonorrhoeae , to the highest of the average amount of said composition hybridized to purified chromosomal DNA of the following six strains of Neisseria meningitidis : l) ATCC 534l4 2) ATCC 534l5 3) ATCC 534l6 4) ATCC 534l7 5) ATCC 534l8 6) ATCC 534l9,
Claim 4
Dependent Claim
59 words
normalized to equal amounts of said purified chromosomal DNA of said six strains of Neisseria gonorrhoeae , to the highest of the average amount of said composition hybridized to purified chromosomal DNA of the following six strains of Neisseria meningitidis : l) ATCC 534l4 2) ATCC 534l5 3) ATCC 534l6 4) ATCC 534l7 5) ATCC 534l8 6) ATCC 534l9,
Claim 5
Dependent Claim
21 words
normalized to equal amounts of said purified chromosomal DNA of said six strains of Neisseria meningitidis , greater than about five.
Claim 6
Dependent Claim
21 words
normalized to equal amounts of said purified chromosomal DNA of said six strains of Neisseria meningitidis , greater than about five.
Claim 7
Dependent Claim
13 words
The composition of Claim l wherein said ratio is greater than about 25.
Claim 8
Dependent Claim
13 words
The composition of Claim 2 wherein said ratio is greater than about 50.
Claim 9
Dependent Claim
77 words
The composition of Claim l wherein said discrete nucleotide sequences are selected from the group consisting of: a. the Neisseria gonorrhoeae DNA insert of ATCC 53409, ATCC 534l0 and ATCC 534ll, and discrete nucleotide subsequences thereof; b. discrete flanking nucleotide sequences of Neisseria gonorrhoeae DNA insert of ATCC 53409, ATCC 534l0 and ATCC 534ll, and discrete nucleotide subsequences thereof; c. mutated discrete nucleotide sequences of any of the foregoing inserts, subsequences and sequences; and d. mixtures thereof.
Claim 10
Dependent Claim
86 words
The composition of Claim l wherein said discrete nucleotide sequences are selected from the group consisting of: a. discrete nucleotide sequences that are capable of hybridizing to the discrete nucleotide sequences of Claim 4; b. discrete flanking nucleotide sequences of any of the foregoing discrete nucleotide sequences, c. discrete nucleotide subsequences of any of the foregoing discrete nucleotide sequences and discrete flanking nucleotide sequences, and d. mutated discrete nucleotide sequences of any of the foregoing discrete nucleotide sequences, discrete flanking nucleotide sequences and discrete nucleotide subsequences.
Claim 11
Dependent Claim
71 words
A nucleotide sequence consisting essentially of nucleotide sequences selected from the group consisting of: a. the Neisseria gonorrhoeae DNA insert of ATCC 53409, ATCC 534l0 and ATCC 534ll, and discrete nucleotide subsequences thereof; b. discrete flanking nucleotide sequences of Neisseria gonorrhoeae DNA insert of ATCC 53409, ATCC 534l0 and ATCC 534ll, and discrete nucleotide subsequences thereof, and c. mutated discrete nucleotide sequences of any of the foregoing inserts, subsequences and sequences.
Claim 12
Dependent Claim
71 words
A nucleotide sequence consisting essentially of nucleotide sequences selected from the group consisting of: a. the Neisseria gonorrhoeae DNA insert of ATCC 53409, ATCC 534l0 and ATCC 534ll, and discrete nucleotide subsequences thereof; b. discrete flanking nucleotide sequences of Neisseria gonorrhoeae DNA insert of ATCC 53409, ATCC 534l0 and ATCC 534ll, and discrete nucleotide subsequences thereof, and c. mutated discrete nucleotide sequences of any of the foregoing inserts, subsequences and sequences.
Claim 13
Dependent Claim
47 words
In a nucleic acid hybridization assay for the detection of Neisseria gonorrhoeae utilizing a polynucleotide probe, the improvement which comprises utilizing as said polynucleotide probe a composition selected from the group consisting of the compositions of Claim l, wherein said composition is labeled with a detectable marker.
Claim 14
Dependent Claim
23 words
The method in accordance with Claim 7 wherein polynucleotide probe is a composition selected from the group consisting of compositions of Claim 4.
Claim 15
Independent Claim
196 words
A method of screening for a nucleotide sequence that is specific for a genetically distinct group which comprises: a. forming a separate test dot on a matrix for each sample that said nucleotide sequences is to be screened against wherein each test dot comprises purified DNA in single-stranded form from one of said samples; b. contacting said test dots with said nucleotide sequence wherein said nucleotide sequences is a single stranded nucleotide sequence insert in a bacteriophage DNA wherein said bacteriophage is a bacteriophage that has a single stranded DNA stage in its life cycle, under hybridizing conditions; c. separating said nucleotide sequence that did not did not hybridize to said test dots from said nucleotide sequence that did hybridize to said test dots; d. contacting said test dots with denatured double-stranded DNA of said bacteriophage wherein said denatured double-stranded DNA of said bacteriophage has a detectable marker attached thereto, under hybridizing conditions; e. separating said double stranded DNA of said bacteriophage that did hybridize to said test dot from those double-stranded DNA of said bacteriophage that did not hybridize to said test dots; and f. detecting said nucleotide sequence by means of said detectable marker.
Claim 16
Independent Claim
196 words
A method of screening for a nucleotide sequence that is specific for a genetically distinct group which comprises: a. forming a separate test dot on a matrix for each sample that said nucleotide sequences is to be screened against wherein each test dot comprises purified DNA in single-stranded form from one of said samples; b. contacting said test dots with said nucleotide sequence wherein said nucleotide sequences is a single stranded nucleotide sequence insert in a bacteriophage DNA wherein said bacteriophage is a bacteriophage that has a single stranded DNA stage in its life cycle, under hybridizing conditions; c. separating said nucleotide sequence that did not did not hybridize to said test dots from said nucleotide sequence that did hybridize to said test dots; d. contacting said test dots with denatured double-stranded DNA of said bacteriophage wherein said denatured double-stranded DNA of said bacteriophage has a detectable marker attached thereto, under hybridizing conditions; e. separating said double stranded DNA of said bacteriophage that did hybridize to said test dot from those double-stranded DNA of said bacteriophage that did not hybridize to said test dots; and f. detecting said nucleotide sequence by means of said detectable marker.
References Cited (Backward Citations)
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