US5170286A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Rapid exchange imaging chamber for stop-flow microscopy
Assignee: United States Department of Health and Human Services
•
Filing: 1991-02-19
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Granted: 1992-12-08
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Expires: 2011-02-19
Abstract
Abstract A specimen chamber comprising a central chamber element sandwiched between a base plate and a cover plate, which base plate and cover plate secure cover slips within recessed areas on opposite surfaces of the central chamber portion which face the base and cover plate. The central chamber element includes a central flow passage, which terminates at opposite ends into tee channels. The sandwiched arrangement of the elements is secured by locking pins, which are insertable into alignment post elements that extend from the base plate though the central chamber element and the cover plate. The locking pins include a plurality of locking cams.
Inventors
- 👤 Joshua J. Zimmerberg
- 👤 James V. Sullivan
- 👤 Peter M. Bungay
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:22
CPC Classifications
G; G02; G02B; G02B21/00; G02B21/34
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
64 words
A specimen chamber comprising a central chamber element sandwiched between a base plate and a cover plate, which base plate and cover plate secure cover slips within recessed areas on opposite surfaces of said central chamber portion which face said base and cover plate, said central chamber element including a central flow passage consisting of a substantially linear channel having opposite terminal ends which
Claim 2
Dependent Claim
64 words
A specimen chamber comprising a central chamber element sandwiched between a base plate and a cover plate, which base plate and cover plate secure cover slips within recessed areas on opposite surfaces of said central chamber portion which face said base and cover plate, said central chamber element including a central flow passage consisting of a substantially linear channel having opposite terminal ends which
Claim 3
Dependent Claim
22 words
A specimen chamber according to claim 1, wherein said central flow passage is in fluid communication with each of said cover slips.
Claim 4
Dependent Claim
36 words
A specimen chamber according to claim 1, wherein each tee channel has opposed ends which terminate into a fluid passage bore, which extends through the surface of the central chamber element which faces the cover plate.
Claim 5
Dependent Claim
24 words
A specimen chamber according to claim 3, further including fluid passage coupling means, which comprise fluid passage lines connectable to said fluid passage bores.
Claim 6
Dependent Claim
40 words
A specimen chamber according to claim 1, wherein said base plate includes a plurality of alignment post elements and each of said central chamber element and cover plate includes a plurality of alignment through-bores for receiving said alignment post elements.
Claim 7
Dependent Claim
46 words
A specimen chamber according to claim 5, further comprising through-bores in each of said alignment post elements and locking pins, which are receivable in said through-bores of said alignment post elements to secure said sandwiched arrangement of said base plate, central chamber element, and cover plate.
Claim 8
Dependent Claim
30 words
A specimen chamber according to claim 6, wherein each of said locking pins is sufficiently long to pass through at least two of said through-bores of said alignment post elements.
Claim 9
Dependent Claim
27 words
A specimen chamber according to claim 7, wherein said locking pins include locking cam means which are adapted to press said cover plate toward said base plate.
Claim 10
Dependent Claim
16 words
A specimen chamber according to claim 1, further including a thermocouple in said central flow passage.
Claim 11
Dependent Claim
22 words
A specimen chamber according to claim 1, further including gasket elements between each of said cover slips and said central chamber element.
Claim 12
Dependent Claim
18 words
A specimen chamber according to claim 1, wherein said central chamber member is made from a plastic material.
Claim 13
Dependent Claim
97 words
A specimen chamber comprising a central chamber element including a central flow passage sandwiched between a base plate and a cover plate, which base plate and cover plate secure cover slips within recessed areas on opposite surfaces of said central chamber portion which face said base and cover plate, said sandwiched arrangement being secured by locking pins which are insertable into alignment post elements which extend though said central chamber element and said cover plate, each of said locking pins being sufficiently long so as to be insertable into at least two of said alignment post elements.
Claim 14
Dependent Claim
97 words
A specimen chamber comprising a central chamber element including a central flow passage sandwiched between a base plate and a cover plate, which base plate and cover plate secure cover slips within recessed areas on opposite surfaces of said central chamber portion which face said base and cover plate, said sandwiched arrangement being secured by locking pins which are insertable into alignment post elements which extend though said central chamber element and said cover plate, each of said locking pins being sufficiently long so as to be insertable into at least two of said alignment post elements.
Claim 15
Dependent Claim
17 words
A specimen chamber according to claim 12, wherein said alignment post elements extend from said base plate.
Claim 16
Dependent Claim
27 words
A specimen chamber according to claim 12, wherein said locking pins include locking cam means which are adapted to press said cover plate toward said base plate.
Claim 17
Dependent Claim
17 words
A specimen chamber according to claim 14, wherein said locking pins include at least two cam elements.
Claim 18
Dependent Claim
15 words
A specimen chamber according to claim 15, wherein said locking pins include three cam elements.
Claim 19
Dependent Claim
19 words
A specimen chamber according to claim 12, wherein said locking pins include pivoting levers to rotate said locking pins.
Claim 20
Dependent Claim
34 words
A specimen chamber according to claim 12, further including fluid passage coupling means connectable to said base plate through said central chamber element and include fluid passage lines connectable with said central flow passage.
Claim 21
Dependent Claim
18 words
A specimen chamber according to claim 12, wherein said central chamber member is made from a plastic material.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-08 | 1996-06-08 | 1996-12-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-08 | 2000-06-08 | 2000-12-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-08 | 2004-06-08 | 2004-12-08 | $7700.00 | Window Closed / Lapsed |