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US5164597A
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Method and apparatus for detecting microorganisms within a liquid product in a sealed vial
Assignee: University of Kentucky Research Foundation
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Filing: 1991-05-06
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Granted: 1992-11-17
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Expires: 2011-05-06
Abstract
Abstract An apparatus and method are provided for the noninvasive and nondestructive detection of microorganisms within a liquid product contained within a sealed vial. The apparatus includes a near-IR light source that produces both incident and reference beams having a wavelength between 800 and 2500 nm and, more preferably, 1100 and 1360 nm. The apparatus also includes an integrating sphere having incident and reference beam ports and a sample window opposite the incident beam port. A detector is mounted in the integrating sphere substantially adjacent the sample window. A substantially U-shaped mirror is provided to hold the vial. The U-shaped mirror is of a size substantially corresponding to the diameter of the vial. In operation, the incident beam is directed through a sample window so as to enter the vial adjacent a sidewall of the mirror. In this way the U-shaped mirror reflects the incident beam so that it passes through the vial three times before returning to the detector. A computer analyzes the resulting signals from the detector.
Inventors
- 👤 Robert A. Lodder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 22:32
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/84; G01N21/88; G01N21/90; G01N21/9018; G01N21/9027; C; C12; C12M; C12M41/00; C12M41/30; C12M41/36; G01N21/17; G01N21/47; G01N21/49; G01N21/51; G01N2021/4704; G01N2021/4709; G01N21/4738; G01N21/474; G01N2021/4742; G01N2201/00; G01N2201/06; G01N2201/065
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
87 words
An apparatus for the noninvasive and non destructive detection of a microorganism with a liquid product contained within a vial, comprising: a light source for producing an incident beam; means for directing said incident beam through said vial and liquid product; means for reflecting said incident beam through said vial and liquid product; means for detecting the reflected incident beam and light scattered by any microorganisms present in said liquid product; and means for analyzing said incident beam and light scattered by any microorganism that is detected.
Claim 2
Independent Claim
87 words
An apparatus for the noninvasive and non destructive detection of a microorganism with a liquid product contained within a vial, comprising: a light source for producing an incident beam; means for directing said incident beam through said vial and liquid product; means for reflecting said incident beam through said vial and liquid product; means for detecting the reflected incident beam and light scattered by any microorganisms present in said liquid product; and means for analyzing said incident beam and light scattered by any microorganism that is detected.
Claim 3
Dependent Claim
23 words
The apparatus set forth in claim 1 wherein said light source produces an incident beam having a wavelength between 800 and 2500 nm.
Claim 4
Dependent Claim
23 words
The apparatus set forth in claim 1, wherein said light source produces an incident beam having a wavelength between 1100 and 1360 nm.
Claim 5
Dependent Claim
33 words
The apparatus set forth in claim 1, wherein said light source also produces a reference beam that is directed to said detecting means so as to compensate for noise and source intensity variations.
Claim 6
Dependent Claim
25 words
The apparatus set forth in claim 1, further including an integrating sphere for collecting scattered light and directing said scattered light toward said detecting means.
Claim 7
Dependent Claim
39 words
The apparatus set forth in claim 5, wherein said integrating sphere includes an incident beam port and a sample window opposite said incident beam port through which said incident beam is directed at said vial and said liquid product.
Claim 8
Dependent Claim
21 words
The apparatus set forth in claim 6, wherein said detecting means is mounted within said integrating sphere adjacent said sample window.
Claim 9
Dependent Claim
17 words
The apparatus set forth in claim 7, wherein said integrating sphere further includes a reference beam port.
Claim 10
Dependent Claim
16 words
The apparatus set forth in claim 8, wherein said reflecting means comprises a substantially U-shaped mirror.
Claim 11
Dependent Claim
26 words
The apparatus set forth in claim 9, wherein said sample window of said integrating sphere is positioned along the open end of said substantially U-shaped mirror.
Claim 12
Dependent Claim
31 words
The apparatus set forth in claim 10, wherein said sample window positioned along the open end of said substantially U-shaped mirror is also adjacent a sidewall of said substantially U-shaped mirror.
Claim 13
Independent Claim
79 words
A method for the noninvasive and nondestructive detection of a microorganism within a liquid product contained with a vial, comprising the steps of: placing the vial adjacent a mirror; directing an incident beam of light into said vial and liquid product; detecting said incident beam of light as well as light scattered by any microorganism present in the liquid product following reflection by said mirror; and analyzing said incident beam and light scattered by any microorganism that is detected.
Claim 14
Independent Claim
79 words
A method for the noninvasive and nondestructive detection of a microorganism within a liquid product contained with a vial, comprising the steps of: placing the vial adjacent a mirror; directing an incident beam of light into said vial and liquid product; detecting said incident beam of light as well as light scattered by any microorganism present in the liquid product following reflection by said mirror; and analyzing said incident beam and light scattered by any microorganism that is detected.
Claim 15
Dependent Claim
22 words
The method set forth in claim 12, wherein said mirror is substantially U-shaped and has a cavity sized to receive said vial.
Claim 16
Dependent Claim
31 words
The method set forth in claim 13, wherein said incident beam is directed into said vial through an open end of said substantially U-shaped mirror adjacent a sidewall of said mirror.
Claim 17
Dependent Claim
23 words
The method set forth in claim 12, including reflecting said incident beam through said vial and liquid product at least twice before detecting.
Claim 18
Dependent Claim
24 words
The method set forth in claim 12, including reflecting said incident beam through said vial and liquid product at least three times before detecting.
Claim 19
Dependent Claim
42 words
The method set forth in claim 12, including steps of identifying multiple discrete scanning planes through said vial and liquid product and directing said incident beam through said multiple discrete scanning planes in order to detect a microorganism in said liquid product.
Claim 20
Dependent Claim
19 words
The method set forth in claim 12, including providing a reference beam to compensate for noise and source-intensity variations.
Claim 21
Dependent Claim
19 words
The method set forth in claim 12, wherein said incident beam has a wavelength between 1100 and 1360 nm.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-17 | 1996-05-18 | 1996-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-17 | 2000-05-18 | 2000-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-17 | 2004-05-18 | 2004-11-17 | $7700.00 | Window Closed / Lapsed |