Patent US5164057A successfully refreshed from Google Patents.
US5164057A
Expired / Lapsed
US
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Electrophoretic matrices and electrophoretic method using same
Assignee: WR Grace and Co Conn
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Filing: 1991-09-19
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Granted: 1992-11-17
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Expires: 2011-09-19
Abstract
Abstract An electrophoretic matrix comprised of at least one water-insolubilized, temperature-responsive polymeric compound having an LCST is disclosed. The electrophoretic matrix can change its hydrophilic/hydrophobic property by changing temperature. A method of using the electrophoretic matrix involves charging a sample onto the matrix and carrying out electrophoresis at a temperature above or below the LCST of the polymeric compound.
Inventors
- 👤 Yuichi Mori
- 👤 Masato Mikami
- 👤 Hiroshi Yoshioka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 14:29
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
14 words
An electrophoretic matrix comprising at least one water-insolubilized temperature-responsive polymeric compound having an LCST.
Claim 2
Dependent Claim
14 words
An electrophoretic matrix comprising at least one water-insolubilized temperature-responsive polymeric compound having an LCST.
Claim 3
Dependent Claim
33 words
The matric of claim 1, wherein said polymeric compound is selected from the group consisting of a poly-N-substituted acrylamide derivative, a poly-N-substituted methacrylamide derivative, their copolymers, polyvinylmethyl ether and partially acetylated polyvinyl alcohol.
Claim 4
Dependent Claim
15 words
The matrix of claim 1, wherein said polymeric compound is provided on a supporting material.
Claim 5
Dependent Claim
18 words
The matrix of claim 3, wherein said polymeric compound is grafted on the surface of said supporting material.
Claim 6
Dependent Claim
13 words
The matrix of claim 3, wherein said supporting material is a porous membrane.
Claim 7
Dependent Claim
52 words
The matrix of claim 5, wherein said porous membrane is selected from the group consisting of a porous acetyl cellulose membrane, a porous polyethylene membrane, a porous polypropylene membrane and a porous polytetrafluoroethylene membrane, a porous polydimethylsiloxane membrane, a porous polyester membrane, a porous polycarbonate membrane and a porous polymethyl methacrylate membrane.
Claim 8
Dependent Claim
21 words
The matrix of claim 6, wherein the number average molecular weight of said temperature-responsive polymeric compound is not greater than 5,000.
Claim 9
Dependent Claim
27 words
The matrix of claim 3, wherein said temperature-responsive polymeric compound is a combination of a plurality of water-insolubilized temperature-responsive polymeric compounds having different LCSTs from one another.
Claim 10
Dependent Claim
27 words
The matrix of claim 1, wherein said temperature-responsive polymeric compound is a combination of a plurality of water-insolubilized temperature-responsive polymeric compounds having different LCSTs from one another.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-17 | 1996-05-18 | 1996-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-17 | 2000-05-18 | 2000-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-17 | 2004-05-18 | 2004-11-17 | $7700.00 | Window Closed / Lapsed |