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US5156019A
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Frozen tissue sectioning apparatus and method
Assignee: Individual
•
Filing: 1990-11-19
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Granted: 1992-10-20
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Expires: 2010-11-19
Abstract
Abstract Disclosed is an apparatus for slicing tissue sections from a frozen specimen for microscopic analysis or the like without allowing tissue debris or dust to become airborne and thereby transport bacteria or viruses from one specimen to other specimens or to an operator. This is achieved by slicing the specimen while submersed in a subzero dielectric liquid and passing a conveyor, such as a statically charged web or film, adjacent the point of sectioning so that the tissue sections and associated tissue debris generated therewith are attracted to the web and adhere thereto. The conveyor transports the tissue sections, and associated debris adhering thereto, through a sterilizer and staining tanks whereafter the tissue is transferred to a slide for microscopic examination.
Inventors
- 👤 James B. McCormick
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:25
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/02; G01N1/04; G01N1/06; G01N1/28; G01N1/42; G01N1/36; G01N2001/362
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
74 words
A cryogenic microtome apparatus for sectioning frozen tissue specimens, comprising: container means adapted to contain a dielectric fluid; microtome means submersed in the dielectric fluid for sectioning a tissue section from a frozen tissue specimen; a conveyor means for conveying the tissue section from said container means; means for mounting said conveyor means immediately adjacent said microtome means; and means for causing adhesion between a tissue section and associated debris and said conveyor means.
Claim 2
Dependent Claim
74 words
A cryogenic microtome apparatus for sectioning frozen tissue specimens, comprising: container means adapted to contain a dielectric fluid; microtome means submersed in the dielectric fluid for sectioning a tissue section from a frozen tissue specimen; a conveyor means for conveying the tissue section from said container means; means for mounting said conveyor means immediately adjacent said microtome means; and means for causing adhesion between a tissue section and associated debris and said conveyor means.
Claim 3
Dependent Claim
33 words
A microtome apparatus in accordance with claim 1 wherein said apparatus includes means for sterilizing the tissue section and associated debris upon transportation from the dielectric fluid while maintaining their chemistry and antigenicity.
Claim 4
Dependent Claim
27 words
A microtome apparatus in accordance with claim 2 wherein, after undergoing sterilization, tissue sections and their associated tissue particles are transported to a mounting station for analysis.
Claim 5
Dependent Claim
27 words
A microtome apparatus in accordance with claim 1 wherein the apparatus includes means for synchronizing the movement of said conveyor with the rate of tissue section generation.
Claim 6
Dependent Claim
42 words
A microtome apparatus in accordance with claim 4 wherein said means for synchronizing the movement of said conveyor with the rate of tissue section generation includes a logic-controlled stepping motor which advances said film a given distance for each tissue section generated.
Claim 7
Dependent Claim
39 words
A microtome apparatus in accordance with claim 1 wherein said means for sectioning organic tissue includes a stationary tissue slicing means and further includes a moving tissue block adjacent each other, said organic tissue being mounted on said block.
Claim 8
Dependent Claim
25 words
A microtome apparatus in accordance with claim 6 wherein an oscillating drive means oscillates the tissue block linearly with respect to said tissue slicing means.
Claim 9
Dependent Claim
16 words
A microtome apparatus in accordance with claim 1 wherein said conveyor means includes a dielectric film.
Claim 10
Dependent Claim
19 words
A microtome apparatus in accordance with claim 8 wherein said dielectric film includes an elongated film web of plastic.
Claim 11
Dependent Claim
20 words
A microtome apparatus in accordance with claim 9 wherein said film and section have opposite electrical surface charges imposed thereon.
Claim 12
Dependent Claim
33 words
A microtome apparatus in accordance with claim 1 wherein said conveyor means includes a continuous, traveling web of dielectric solid passing adjacent said microtome means at a point on its path of travel.
Claim 13
Dependent Claim
29 words
A microtome apparatus in accordance with claim 2 wherein the apparatus includes a series of tanks for receiving staining and fixing agent and means for passing said conveyor therethrough.
Claim 14
Dependent Claim
36 words
A microtome apparatus in accordance with claim 2 wherein said means for sterilizing said tissue sections adhering to the dielectric solid includes an oxidizing lamp for oxidizing tissue passing in close proximity to the oxidizing lamp.
Claim 15
Dependent Claim
32 words
A microtome apparatus in accordance with claim 1 including a means for transfer attaching the tissue section from the plastic web to a glass slide with a U.V. polymerized resin mounting material.
Claim 16
Dependent Claim
29 words
A microtome apparatus in accordance with claim 3 including gas- and liquid-phase processing means for processing said tissue prior to said transportation thereof to a mounting station for analysis.
Claim 17
Dependent Claim
25 words
A microtome apparatus in accordance with claim 1 wherein said dielectric fluid is maintained between approximately -40° C. and 0° C., preferably at -20° C.
Claim 18
Independent Claim
71 words
A method for the preparation of tissue sections comprising: sectioning a tissue specimen at a sectioning station to provide a tissue section and associated debris; adhering said section and debris to the surface of a conveyor; conveying said section and associated debris to a mounting station remote from said sectioning station; removing said tissue section from said conveyor at said mounting station; and mounting said tissue to provide a microscopic slide.
Claim 19
Independent Claim
71 words
A method for the preparation of tissue sections comprising: sectioning a tissue specimen at a sectioning station to provide a tissue section and associated debris; adhering said section and debris to the surface of a conveyor; conveying said section and associated debris to a mounting station remote from said sectioning station; removing said tissue section from said conveyor at said mounting station; and mounting said tissue to provide a microscopic slide.
Claim 20
Dependent Claim
17 words
A method in accordance with claim 17 wherein the sectioning station is immersed in a dielectric fluid.
Claim 21
Dependent Claim
13 words
A method in accordance with claim 17 wherein the tissue specimen is frozen.
Claim 22
Dependent Claim
24 words
A method in accordance with claim 18 wherein the dielectric fluid is at a temperature of between about -10° C. and about -40° C.
Claim 23
Independent Claim
103 words
A method for sectioning organic tissue under cryogenic conditions, comprising: submersing a frozen tissue specimen to be sectioned in a subzero dielectric liquid means; passing a dielectric film in proximity with said frozen tissue specimen; imposing an electrical surface charge to one of said dielectric film and said organic tissue such that as tissue is sectioned, the tissue section and its associated tissue particles electrostatically adhere to the dielectric film passing in proximity with the tissue; sectioning said tissue by sectioning means while the tissue is submersed in said dielectric liquid; and transporting the sectioned tissue and tissue particles from the dielectric liquid.
Claim 24
Independent Claim
103 words
A method for sectioning organic tissue under cryogenic conditions, comprising: submersing a frozen tissue specimen to be sectioned in a subzero dielectric liquid means; passing a dielectric film in proximity with said frozen tissue specimen; imposing an electrical surface charge to one of said dielectric film and said organic tissue such that as tissue is sectioned, the tissue section and its associated tissue particles electrostatically adhere to the dielectric film passing in proximity with the tissue; sectioning said tissue by sectioning means while the tissue is submersed in said dielectric liquid; and transporting the sectioned tissue and tissue particles from the dielectric liquid.
Claim 25
Dependent Claim
23 words
A method in accordance with claim 21 including the step of sterilizing the tissue sections while they are electrostatically adhered to the film.
Claim 26
Dependent Claim
22 words
A method in accordance with claim 22 including the step of transporting the sterilized tissue sections to a mounting station for viewing.
Claim 27
Dependent Claim
20 words
A method in accordance with claim 21 including the step of passing tissue sections through gas- and liquid-phase processing means.
Claim 28
Dependent Claim
25 words
A method in accordance with claim 21 including the step of synchronizing the movement of said dielectric solid with the rate of tissue section generation.
References Cited (Backward Citations)
US3204424A
Cited
US3233965A
Cited
US3272348A
Cited
US3462969A
Cited
US3491638A
Cited
US3664412A
Cited
DE2141129A1
Cited
SU426164A1
Cited
US4548051A
Cited
US4979376A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-10-20 | 1996-04-20 | 1996-10-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-10-20 | 2000-04-20 | 2000-10-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-10-20 | 2004-04-20 | 2004-10-20 | $7700.00 | Window Closed / Lapsed |