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US5153004A
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Freezing and thawing of erythrocytes
Assignee: Terumo BCT Inc
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Filing: 1990-04-05
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Granted: 1992-10-06
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Expires: 2010-04-05
Abstract
Abstract A process and medium are disclosed for the freezing of red blood cells which comprises the use of solutions including monosaccharide hexoses and pentoses, and/or biocompatible amphipathic polymers to permit the thawing without washing to produce viable red blood cells.
Inventors
- 👤 Raymond P. Goodrich, Jr.
- 👤 Christine M. Williams
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 14:45
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/18
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
54 words
A process for storing a composition consisting essentially of washed human erythrocytes having a cell membrane, comprising immersing washed erythrocytes in a solution which includes a monosaccharide, which is capable of permeating the membrane of the erythrocytes, in a concentration of from about 0.2 to about 4 molar; and freezing said solution and erythrocytes.
Claim 2
Dependent Claim
54 words
A process for storing a composition consisting essentially of washed human erythrocytes having a cell membrane, comprising immersing washed erythrocytes in a solution which includes a monosaccharide, which is capable of permeating the membrane of the erythrocytes, in a concentration of from about 0.2 to about 4 molar; and freezing said solution and erythrocytes.
Claim 3
Dependent Claim
16 words
A process according to claim 1 further comprising the step of thawing said solution and erythrocytes.
Claim 4
Dependent Claim
18 words
The process of claim 1 wherein the monosaccharide is selected from the group consisting of pentose and hexose.
Claim 5
Dependent Claim
23 words
The process of claim 1 or 2 wherein the monosaccharide is selected from the group consisting of xylose, glucose, ribose, mannose and fructose.
Claim 6
Dependent Claim
20 words
The process of claim 4 wherein the monosaccharide is present in the solution in a concentration of about 2 molar.
Claim 7
Dependent Claim
80 words
A process for storing a composition consisting essentially of washed erythrocytes, comprising: immersing washed erythrocytes in a water solution which includes: a monosaccharide which is present in the solution in a concentration of from about 0.5 molar up to about 4 molar, and an amphipathic polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.1 millimolar up to saturation in the solution; and freezing said solution and erythrocytes.
Claim 8
Dependent Claim
80 words
A process for storing a composition consisting essentially of washed erythrocytes, comprising: immersing washed erythrocytes in a water solution which includes: a monosaccharide which is present in the solution in a concentration of from about 0.5 molar up to about 4 molar, and an amphipathic polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.1 millimolar up to saturation in the solution; and freezing said solution and erythrocytes.
Claim 9
Dependent Claim
16 words
A process according to claim 6 further comprising the step of thawing said solution and erythrocytes.
Claim 10
Dependent Claim
20 words
The process of claim 6 or 7 wherein the monosaccharide is selected from the group consisting of pentoses and hexoses.
Claim 11
Dependent Claim
23 words
The process of claim 6 or 7 wherein the monosaccharide is selected from the group consisting of xylose, glucose, ribose, mannose and fructose.
Claim 12
Dependent Claim
20 words
The process of claim 6 or 7 wherein the polymer is selected from the group consisting of polyvinylpyrrolidone and dextran.
Claim 13
Dependent Claim
12 words
The process of claim 6 or 7 wherein the polymer is polyvinylpyrrolidone.
Claim 14
Dependent Claim
15 words
A composition comprising reconstituted erythrocytes produced according to the process of claim 2 or 7.
Claim 15
Dependent Claim
15 words
A composition of frozen erythrocytes produced according to the process of claim 1 or 6.
Claim 16
Dependent Claim
19 words
A process according to claim 6 wherein said amphipathic polymer has a molecular weight of at least about 2.5K.
References Cited (Backward Citations)
US4980277A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-10-06 | 1996-04-06 | 1996-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-10-06 | 2000-04-06 | 2000-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-10-06 | 2004-04-06 | 2004-10-06 | $7700.00 | Window Closed / Lapsed |