US5151355A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of high purity thrombin
Assignee: Warner Lambert Pottery Road Ltd
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Filing: 1991-01-23
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Granted: 1992-09-29
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Expires: 2011-01-23
Abstract
Abstract A liquid thrombin preparation is prepared by reacting a unit of prothrombin with less than 5 units of thromboplastin in the presence of calcium, contacting the resultant thrombin with a phosphate buffer, and diluting and filtering the suspension. The filtrate is then applied sequentially to an anion-exchange agarose column and a cation-exchange agarose column and the thrombin fraction is step-wise eluted from the latter column with phosphate buffered saline. Liquid thrombin preparations thereby obtained have specific activities greater than 1,600 U/mg and can be used in haemostasis.
Inventors
- 👤 Carmel Crowley
- 👤 Timothy G. Sheehan
- 👤 Frederick G. Ward
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:45
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6429; A; A61; A61P; A61P7/00; A61P7/04; C12Y; C12Y304/00; C12Y304/21; C12Y304/21005
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
96 words
A process for the production of a liquid thrombin preparation, which process comprises reacting each unit of prothrombin measured as N.I.H. units of thrombin produced, with less than 5 units of thromboplastin, measured as N.I.H. units of thrombin produced, in the presence of calcium to produce thrombin contacting the resultant thrombin with a phosphate buffer to produce a suspension diluting and filtering the suspension to produce a filtrate applying the filtrate sequentially to an anion-exchange agarose column and a cation-exchange agarose column and step-wise eluting a thrombin fraction from the cation-exchange column with phosphate buffered saline.
Claim 2
Dependent Claim
96 words
A process for the production of a liquid thrombin preparation, which process comprises reacting each unit of prothrombin measured as N.I.H. units of thrombin produced, with less than 5 units of thromboplastin, measured as N.I.H. units of thrombin produced, in the presence of calcium to produce thrombin contacting the resultant thrombin with a phosphate buffer to produce a suspension diluting and filtering the suspension to produce a filtrate applying the filtrate sequentially to an anion-exchange agarose column and a cation-exchange agarose column and step-wise eluting a thrombin fraction from the cation-exchange column with phosphate buffered saline.
Claim 3
Dependent Claim
18 words
A process according to claim 1, wherein the amount of thromboplastin used is between 2 and 4 units.
Claim 4
Dependent Claim
19 words
A process according to claim 1, wherein the amount of thromboplastin used is of the order of 3 units.
Claim 5
Dependent Claim
19 words
A process according to claim 1, wherein the phosphate buffer used is a 0.5-1.5M phosphate buffer at pH 6.0-7.0.
Claim 6
Dependent Claim
19 words
A process according to claim 1, wherein the phosphate buffer used is a 1.0M phosphate buffer at pH 6.5.
Claim 7
Dependent Claim
20 words
A process according to claim 1, wherein the step-wise elution of the cation exchange agarose column is a two-step elution.
Claim 8
Dependent Claim
27 words
A process according to claim 1, wherein the cation exchange agarose column is sequentially eluted with i) 0.16-0.18M phosphate buffered saline and ii) 0.26-0.30M phosphate buffered saline.
Claim 9
Dependent Claim
27 words
A process according to claim 1, wherein the cation exchange agarose column is sequentially eluted with i) 0.17M phosphate buffered saline and ii) 0.28M phosphate buffered saline.
References Cited (Backward Citations)
DE265329C
Cited
US4286056A
Cited
US4363319A
Cited
US4380511A
Cited
US4470969A
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US4473553A
Cited
US4736018A
Cited
US4637932A
Cited
US4696812A
Cited
US4965203A
Cited
SU1527261A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-29 | 1996-03-30 | 1996-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-29 | 2000-03-30 | 2000-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-29 | 2004-03-30 | 2004-09-29 | $7700.00 | Window Closed / Lapsed |