US4965203A
Expired / Lapsed
US
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Purified thrombin preparations
Assignee: Warner Lambert Co LLC
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Filing: 1989-06-13
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Granted: 1990-10-23
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Expires: 2009-06-13
Abstract
Abstract Improved thrombin formulations, stable at room temperature using stabilizing quantities of a polyol and a buffer at specific pHs are described wherein crude thrombin is first purified by two-stage ion exchange chromatography.
Inventors
- 👤 Steven B. Silbering
- 👤 Rowland P. Blythe, Jr.
- 👤 Russell U. Nesbitt, Jr.
- 👤 Mahdi B. Fawzi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 23:47
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6429; A; A61; A61K; A61K47/00; A61K47/02; A61K47/06; A61K47/08; A61K47/10; A61K47/12; C12Y; C12Y304/00; C12Y304/21; C12Y304/21005; A61K38/00; Y; Y10; Y10S; Y10S128/00; Y10S128/22
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
93 words
In a method of preparing a stable thrombin composition consisting essentially of thrombin, 25-50% of a polyol and a buffer of acetate or phosphate ions, the improvement consisting of further purification of the thrombin comprising: (a) adsorbing the thrombin on a DEAE agarose column, (b) eluting the thrombin from the said DEAE agarose column with 0.45 M buffer in 0.45 M NaCl, (c) adsorbing the eluate of step (b) on a CM agarose column and, (d) eluting the thrombin from the said CM agarose column with 0.45 M buffer in 0.45 M NaCl.
Claim 2
Dependent Claim
93 words
In a method of preparing a stable thrombin composition consisting essentially of thrombin, 25-50% of a polyol and a buffer of acetate or phosphate ions, the improvement consisting of further purification of the thrombin comprising: (a) adsorbing the thrombin on a DEAE agarose column, (b) eluting the thrombin from the said DEAE agarose column with 0.45 M buffer in 0.45 M NaCl, (c) adsorbing the eluate of step (b) on a CM agarose column and, (d) eluting the thrombin from the said CM agarose column with 0.45 M buffer in 0.45 M NaCl.
Claim 3
Dependent Claim
10 words
The method of claim 1 wherein the polyol is glycerol.
Claim 4
Dependent Claim
28 words
The method of claim 1 wherein the buffer of the said stable thrombin composition is a phosphate buffer and the final pH is about 6.0 to about 6.5.
Claim 5
Dependent Claim
28 words
The method of claim 1 wherein the buffer of the said stable thrombin composition is an acetate buffer and the final pH is about 5.0 to about 5.5.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-22 | 1994-04-23 | 1994-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-22 | 1998-04-23 | 1998-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-22 | 2002-04-23 | 2002-10-23 | $7700.00 | Window Closed / Lapsed |