US5149647A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for extracting pure fractions of lactoperoxidase and lactoferrin from milk serum
Assignee: Campina Melkune BV
•
Filing: 1988-11-25
•
Granted: 1992-09-22
•
Expires: 2008-11-25
Abstract
Abstract PCT No. PCT/SE88/00643 Sec. 371 Date May 24, 1990 Sec. 102(e) Date May 24, 1990 PCT Filed Nov. 25, 1988 PCT Pub. No. WO89/04608 PCT Pub. Date Jun. 1, 1989.A process for extracting pure fractions of lactoperoxidase and lactoferrin from milk serum is described. The milk serum is microfiltered and passed through a strong cation exchanger at a high rate of flow for selective adsorption of lactoperoxidase and lactoferrin, and then the lactoperoxidase and lactoferrin are eluted successively and selectively with saline solutions having different concentrations.
Inventors
- 👤 Hans Burling
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 17:58
CPC Classifications
A; A23; A23C; A23C9/00; A23C9/14; A23C9/146; A23C9/1465; A23C9/142; A23C9/1425; A23J; A23J1/00; A23J1/20; A23J1/205; A23V; A23V2002/00; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
88 words
A process for extracting pure fractions of lactoperoxidase and lactoferrin from milk serum, comprising initially microfiltering the milk serum, then passing it through a bed of a fast flow type strong cation exchanger at a high rate of flow of about 1-1.5 bed volumes/minute for selective adsorption of lactoperoxidase and lactoferrin, and then successively and selectively eluting the lactoperoxidase with a saline solution having a concentration of 0.10-0.4 M at a pH of about 6.5 and the lactoferrin with a saline solution having a concentration of 0.5-2 M.
Claim 2
Dependent Claim
88 words
A process for extracting pure fractions of lactoperoxidase and lactoferrin from milk serum, comprising initially microfiltering the milk serum, then passing it through a bed of a fast flow type strong cation exchanger at a high rate of flow of about 1-1.5 bed volumes/minute for selective adsorption of lactoperoxidase and lactoferrin, and then successively and selectively eluting the lactoperoxidase with a saline solution having a concentration of 0.10-0.4 M at a pH of about 6.5 and the lactoferrin with a saline solution having a concentration of 0.5-2 M.
Claim 3
Dependent Claim
29 words
The process as claimed in claim 1, where prior to the elution of lactoperoxidase, the cation exchanger is eluted with a saline solution having a concentration of 0.01-0.15 M.
Claim 4
Dependent Claim
31 words
The process as claimed in claim 1 or 2, wherein that the pH of the milk serum is adjusted to 5.9-9.0, preferably about 6.5, before being passed through the cation exchanger.
Claim 5
Dependent Claim
22 words
The process as claimed claim 1 wherein the microfiltration is carried out in a microfilter having a pore diameter of 0.10-2 μm.
Claim 6
Dependent Claim
21 words
The process as claimed claim 1 wherein the saline solutions with eluted lactoperoxidase and lactoferrin, respectively, are concentrated, desalted and freeze-dried.
Claim 7
Dependent Claim
23 words
The process as claimed in claim 2, wherein the microfiltration is carried out in a microfilter having a pore diameter of 0.10-2 μm.
Claim 8
Dependent Claim
23 words
The process as claimed in claim 3, wherein the microfiltration is carried out in a microfilter having a pore diameter of 0.10-2 μm.
Claim 9
Dependent Claim
23 words
The process as claimed in claim 1, wherein the microfiltration is carried out in a microfilter having a pore diameter of 0.4-1.5 μm.
Claim 10
Dependent Claim
23 words
The process as claimed in claim 2, wherein the microfiltration is carried out in a microfilter having a pore diameter of 0.4-1.5 μm.
Claim 11
Dependent Claim
23 words
The process as claimed in claim 3, wherein the microfiltration is carried out in a microfilter having a bore diameter of 0.4-1.5 μm.
Claim 12
Dependent Claim
22 words
The process as claimed in claim 2, wherein the saline solutions with eluted lactoperoxidase and lactoferrin, respectively, are concentrated, desalted and freeze-dried.
Claim 13
Dependent Claim
22 words
The process as claimed in claim 3, wherein the saline solutions with eluted lactoperoxidase and lactoferrin, respectively, are concentrated, desalted and freeze-dried.
Claim 14
Dependent Claim
22 words
The process as claimed in claim 4, wherein the saline solutions with eluted lactoperoxidase and lactoferrin, respectively, are concentrated, desalted and freeze-dried.
Claim 15
Dependent Claim
21 words
The process of claim 2 wherein said salt solution is a solution of an inorganic alkali, alkaline earth or ammonium salt.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-22 | 1996-03-23 | 1996-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-22 | 2000-03-23 | 2000-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-22 | 2004-03-23 | 2004-09-22 | $7700.00 | Window Closed / Lapsed |