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US5145783A
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Glyphosate-tolerant 5-endolpyruvyl-3-phosphoshikimate synthase
Assignee: Monsanto Technology LLC
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Filing: 1990-07-09
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Granted: 1992-09-08
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Expires: 2010-07-09
Abstract
Abstract Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphosate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a conserved sequence found between positions 80 and 120 in the mature wild-type EPSP synthase.
Inventors
- 👤 Ganesh M. Kishore
- 👤 Dilip M. Shah
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:06
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8274; C12N15/8275; C12N9/00; C12N9/10
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
31 words
A plant transformation vector comprising a gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase, said EPSP synthase having the sequence: --L--G--N--A--A--T--A-- between positions 80 and 120 of the mature EPSP synthase sequence.
Claim 2
Dependent Claim
31 words
A plant transformation vector comprising a gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase, said EPSP synthase having the sequence: --L--G--N--A--A--T--A-- between positions 80 and 120 of the mature EPSP synthase sequence.
Claim 3
Dependent Claim
14 words
A vector of claim 1 comprising a gene encoding a glyphosate-tolerant plant EPSP synthase.
Claim 4
Dependent Claim
14 words
A vector of claim 1 comprising a gene encoding a glyphosate-tolerant bacterial EPSP synthase.
Claim 5
Dependent Claim
14 words
A vector of claim 1 comprising a gene encoding a glyphosate-tolerant fungal EPSP synthase.
Claim 6
Dependent Claim
33 words
A transformed plant cell containing a gene encoding a glyphosate-tolerant plant EPSP synthase enzyme, which enzyme contains the amino acid sequence: --L--L--N--A--A--T--A-- between positions 80 and 120 in the mature EPSP synthase sequence.
Claim 7
Dependent Claim
33 words
A transformed plant cell containing a gene encoding a glyphosate-tolerant plant EPSP synthase enzyme, which enzyme contains the amino acid sequence: --L--L--N--A--A--T--A-- between positions 80 and 120 in the mature EPSP synthase sequence.
Claim 8
Dependent Claim
29 words
A transformed plant cell of claim 5 selected from the group consisting of tomato, tobacco, oil seed rape, flax, soybean, sunflower, sugar beet, alfalfa, cotton, rice and maize cells.
Claim 9
Dependent Claim
8 words
A transformed cell of claim 5 from tomato.
Claim 10
Dependent Claim
8 words
A transformed cell of claim 5 from tobacco.
Claim 11
Dependent Claim
10 words
A transformed cell of claim 5 from oil seed rape.
Claim 12
Dependent Claim
8 words
A transformed cell of claim 5 from flax.
Claim 13
Dependent Claim
8 words
A transformed cell of claim 5 from soybean.
Claim 14
Dependent Claim
8 words
A transformed cell of claim 5 from sunflower.
Claim 15
Dependent Claim
9 words
A transformed cell of claim 5 from sugar beet.
Claim 16
Dependent Claim
8 words
A transformed cell of claim 5 from alfalfa.
Claim 17
Dependent Claim
8 words
A transformed cell of claim 5 from maize.
Claim 18
Dependent Claim
9 words
A plant comprising transformed plant cells of claim 5.
Claim 19
Dependent Claim
11 words
A plant of claim 16 in which the plant is tomato.
Claim 20
Dependent Claim
11 words
A plant of claim 16 in which the plant is tobacco.
Claim 21
Dependent Claim
13 words
A plant of claim 16 in which the plant is oil seed rape.
Claim 22
Dependent Claim
11 words
A plant of claim 16 in which the plant is flax.
Claim 23
Dependent Claim
11 words
A plant of claim 16 in which the plant is sunflower.
Claim 24
Dependent Claim
12 words
A plant of claim 16 in which the plant is sugar beet.
Claim 25
Dependent Claim
11 words
A plant of claim 16 in which the plant is alfalfa.
Claim 26
Dependent Claim
9 words
A seed produced by a plant of claim 16.
Claim 27
Dependent Claim
11 words
A seed of claim 24 in which the plant is tomato.
Claim 28
Dependent Claim
11 words
A seed of claim 24 in which the plant is tobacco.
Claim 29
Dependent Claim
13 words
A seed of claim 24 in which the plant is oil seed rape.
Claim 30
Dependent Claim
11 words
A seed of claim 24 in which the plant is flax.
Claim 31
Dependent Claim
11 words
A seed of claim 24 in which the plant is sunflower.
Claim 32
Dependent Claim
12 words
A seed of claim 24 in which the plant is sugar beet.
Claim 33
Dependent Claim
11 words
A seed of claim 24 in which the plant is alfalfa.
References Cited (Backward Citations)
US4971908A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1995-09-08 | 1996-03-09 | 1996-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-08 | 2000-03-09 | 2000-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-08 | 2004-03-09 | 2004-09-08 | $7700.00 | Window Closed / Lapsed |