Patent CH359264A successfully refreshed from Google Patents.
Patent US5128243A successfully refreshed from Google Patents.
US5128243A
Expired / Lapsed
US
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Assay for the detection of beet sugar adulteration of food products
Assignee: University of South Florida St. Petersburg
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Filing: 1990-06-28
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Granted: 1992-07-07
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Expires: 2010-06-28
Abstract
Abstract An immunoassay for the detection of beet sugar adulteration of sugar-containing materials such as fruit juices and fruit juice concentrates utilizes antibodies to proteinaceous components of beet sugar. These proteinaceous components have been found in all samples of beet sugar, tested, regardless of source or degree of refinement; these proteinaceous components have also been found in both crystal and invert sugars.
Inventors
- 👤 Robert L. Potter
- 👤 Richard L. L. Mansell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 08:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5308; G01N33/68; Y; Y10; Y10S; Y10S436/00; Y10S436/815
Claims Analysis
Total Claims: 48
Claim 1
Independent Claim
92 words
An antibody raised against a proteinaceous component of beet sugar, said proteinaceous component of beet sugar comprising at least one proteinaceous material, found in beet sugar, selected from the group consisting of those proteinaceous materials, found in beet sugar, having a molecular weight as determined by carrying our electrophoresis on said proteinaceous material and comparing its mobility with that of marker proteins of known molecular weight, of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons, and about 16,000 daltons.
Claim 2
Dependent Claim
92 words
An antibody raised against a proteinaceous component of beet sugar, said proteinaceous component of beet sugar comprising at least one proteinaceous material, found in beet sugar, selected from the group consisting of those proteinaceous materials, found in beet sugar, having a molecular weight as determined by carrying our electrophoresis on said proteinaceous material and comparing its mobility with that of marker proteins of known molecular weight, of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons, and about 16,000 daltons.
Claim 3
Dependent Claim
28 words
The antibody according to claim 1, wherein said proteinaceous component of beet sugar comprises more than about 50% by weight of amino acids linked by acid labile bonds.
Claim 4
Dependent Claim
18 words
The antibody according to claim 2, wherein said proteinaceous component is rich in alanine, serine, glycine and proline.
Claim 5
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 61,000 daltons.
Claim 6
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 45,000 daltons.
Claim 7
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 39,000 daltons.
Claim 8
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 36,000 daltons.
Claim 9
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 30,000 daltons.
Claim 10
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 27,000 daltons.
Claim 11
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 21,000 daltons.
Claim 12
Dependent Claim
29 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a proteinaceous material, found in beet sugar, having a molecular weight of about 16,000 daltons.
Claim 13
Dependent Claim
52 words
The antibody according to claim 2, wherein said proteinaceous component of beet sugar comprises a mixture of proteinaceous materials, found in beet sugar, having molecular weights of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons and about 16,000 daltons.
Claim 14
Dependent Claim
14 words
The antibody according to claim 1, wherein said beet sugar is a crystalline sugar.
Claim 15
Dependent Claim
15 words
The antibody according to claim 1, wherein said beet sugar is at least partially inverted.
Claim 16
Dependent Claim
24 words
The antibody according to claim 14, wherein said at least partially inverted beet sugar has a degree of inversion of about 50 to 90%.
Claim 17
Independent Claim
63 words
A method of detecting the presence of beet sugar in a sugar-containing material, said method comprising: treating a test sample of said sugar-containing material to selectively recover proteinaceous materials therefrom to form a protein test sample; contacting said protein test sample with antibody raised against a proteinaceous component of beet sugar; and detecting the formation of complexes of said proteinaceous component and antibody.
Claim 18
Independent Claim
63 words
A method of detecting the presence of beet sugar in a sugar-containing material, said method comprising: treating a test sample of said sugar-containing material to selectively recover proteinaceous materials therefrom to form a protein test sample; contacting said protein test sample with antibody raised against a proteinaceous component of beet sugar; and detecting the formation of complexes of said proteinaceous component and antibody.
Claim 19
Dependent Claim
45 words
The method of claim 16, wherein said treatment of said test sample to selectively recover proteinaceous materials therefrom comprises contacting said test sample with a weak anion exchange resin and then eluting the material absorbed on said weak anion exchange resin to form an eluant.
Claim 20
Dependent Claim
15 words
The method of claim 17, further comprising ultrafiltration of said eluant to produce a retentate.
Claim 21
Dependent Claim
23 words
The method of claim 18, wherein said ultrafiltration produces a retentate containing materials having a molecular weight of greater than about 10,000 daltons.
Claim 22
Dependent Claim
13 words
The method of claim 18, further comprising concentration of said retentate by centrifugation.
Claim 23
Dependent Claim
88 words
The method of claim 16, wherein said proteinaceous component of beet sugar comprises at least one proteinaceous material, found in beet sugar, selected from the group consisting of those proteinaceous materials, found in beet sugar, having a molecular weight, as determined by carrying out electrophoresis on said proteinaceous material and comparing its movement with that of marker proteins of known molecular weight, of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons and about 16,000 daltons.
Claim 24
Dependent Claim
27 words
The method of claim 21, wherein said proteinaceous component of beet sugar comprises more than about 50% by weight of amino acids linked by acid labile bonds.
Claim 25
Dependent Claim
17 words
The method of claim 22, wherein said proteinaceous component is rich in alanine, serine, glycine and proline.
Claim 26
Dependent Claim
51 words
The method of claim 21, wherein said proteinaceous component of beet sugar comprises a mixture of proteinaceous materials, found in beet sugar, having molecular weights of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons and about 16,000 daltons.
Claim 27
Dependent Claim
17 words
The method of claim 16, wherein said detection of said complexes is effected by enzyme-linked immunosorbent assay.
Claim 28
Dependent Claim
29 words
The method of claim 16, further comprising removing at least a major portion of the sugar present in a sample of said sugar-containing material to form said test sample.
Claim 29
Dependent Claim
25 words
The method of claim 26, wherein at least 80% of the sugar present in said sample of sugar-containing material is removed from said test sample.
Claim 30
Dependent Claim
26 words
The method of claim 27, wherein at least 85% of the sugar present in said sample of sugar-containing material is removed to form said test sample.
Claim 31
Dependent Claim
19 words
The method of claim 16, wherein said removal of said sample of said sugar-containing material is effected by dialysis.
Claim 32
Dependent Claim
21 words
The method of claim 29, wherein said dialysis removes materials having a molecular weight of not more than about 6,000 daltons.
Claim 33
Dependent Claim
27 words
The method of claim 26, further comprising forming an aqueous sugar solution of said sugar-containing material prior to removal of said major portion of the sugar therefrom.
Claim 34
Dependent Claim
28 words
The method of claim 31, wherein said aqueous sugar solution is filtered to remove particulate material therefrom prior to removal of said major portion of the sugar therefrom.
Claim 35
Dependent Claim
20 words
The method of claim 32, wherein said filtration removes particulate material having an average diameter greater than about 0.2 micron.
Claim 36
Dependent Claim
17 words
The method of claim 16, wherein said sugar-containing material is a fruit juice or fruit juice concentrate.
Claim 37
Dependent Claim
25 words
The method of claim 34, further comprising filtering said fruit juice or fruit juice concentrate prior to said treatment to selectively recover proteinaceous materials therefrom.
Claim 38
Dependent Claim
20 words
The method of claim 35, wherein said filtration removes particulate material having an average diameter greater than about 0.2 micron.
Claim 39
Dependent Claim
16 words
The method of claim 34, wherein said sugar-containing material is apple juice or apply juice concentrate.
Claim 40
Dependent Claim
16 words
The method of claim 34, wherein said sugar-containing material is orange juice or orange juice concentrate.
Claim 41
Dependent Claim
93 words
Rabbit IgG specifically binds to a proteinaceous component of beet sugar, said proteinaceous component of beet sugar comprising at least one proteinaceous material, found in beet sugar, selected from the group consisting of those proteinaceous materials, found in beet sugar, having a molecular weight, as determined by carrying out electrophoresis on said proteinaceous material and comparing its mobility with that of marker proteins of known molecular weight, of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons and about 16,000 daltons.
Claim 42
Dependent Claim
93 words
Rabbit IgG specifically binds to a proteinaceous component of beet sugar, said proteinaceous component of beet sugar comprising at least one proteinaceous material, found in beet sugar, selected from the group consisting of those proteinaceous materials, found in beet sugar, having a molecular weight, as determined by carrying out electrophoresis on said proteinaceous material and comparing its mobility with that of marker proteins of known molecular weight, of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons and about 16,000 daltons.
Claim 43
Dependent Claim
29 words
The rabbit IgG according to claim 39, wherein said proteinaceous component of beet sugar comprises more than about 50% by weight of amino acids linked by acid labile bonds.
Claim 44
Dependent Claim
19 words
The rabbit IgG according to claim 40, wherein said proteinaceous component is rich in alanine, serine, glycine and proline.
Claim 45
Dependent Claim
125 words
A test kit for the detection of beet sugar in a sugar-containing material comprising: a predetermined quantity of a first mammalian IgG specifically binds to a proteinaceous component of beet sugar, said proteinaceous component of beet sugar comprising at least one proteinaceous material, found in beet sugar, selected from the group consisting of those proteinaceous materials, found in beet sugar, having a molecular weight, as determined by carrying out electrophoresis on said proteinaceous material and comparing its mobility with that of marker proteins of known molecular weight, of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons and about 16,000 daltons; and a predetermined quantity of a labelled second mammalian anti-first mammal IgG.
Claim 46
Dependent Claim
125 words
A test kit for the detection of beet sugar in a sugar-containing material comprising: a predetermined quantity of a first mammalian IgG specifically binds to a proteinaceous component of beet sugar, said proteinaceous component of beet sugar comprising at least one proteinaceous material, found in beet sugar, selected from the group consisting of those proteinaceous materials, found in beet sugar, having a molecular weight, as determined by carrying out electrophoresis on said proteinaceous material and comparing its mobility with that of marker proteins of known molecular weight, of about 61,000 daltons, about 45,000 daltons, about 39,000 daltons, about 36,000 daltons, about 30,000 daltons, about 27,000 daltons, about 21,000 daltons and about 16,000 daltons; and a predetermined quantity of a labelled second mammalian anti-first mammal IgG.
Claim 47
Dependent Claim
27 words
The test kit according to claim 42, wherein said mammalian IgG is rabbit IgG and said labelled second mammalian anti-first mammal IgG is labelled goat anti-rabbit IgG.
Claim 48
Dependent Claim
19 words
The test kit according to claim 43, wherein said labelled goat anti-rabbit IgG is goat anti-rabbit IgG-alkaline phosphatase conjugate.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-07-07 | 1996-01-06 | 1996-07-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-07-07 | 2000-01-06 | 2000-07-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-07-07 | 2004-01-06 | 2004-07-07 | $7700.00 | Window Closed / Lapsed |