Patent US780856A successfully refreshed from Google Patents.
Patent US1374783A successfully refreshed from Google Patents.
US5118623A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Bleach stable enzymes
Assignee: Danisco US Inc
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Filing: 1988-05-27
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Granted: 1992-06-02
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Expires: 2008-05-27
Abstract
Abstract The methods of the invention can be used to identify sites of cleavage of an enzyme in the presence of hypochlorite. It has been found that such cleavage occurs at tryptophan. Chemical modifications or genetic manipulation to change or delete tryptophan can be done to produce a more stable enzyme which retains activity in the presence of hypochlorite. The invention is particularly applicable to alkaline proteases which are useful in detergent compositions.
Inventors
- 👤 George Boguslawski
- 👤 John W. Shultz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 21:01
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/395; C11D3/3953; C12N15/00; C12N15/09; C12N15/10
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
32 words
An improved Bacillus licheniformis alkaline protease, wherein at least one tryptophan has been genetically substituted by tyrosine or phenylalanine, whereby said protease is made stable against cleavage by hypochlorite at said residue.
Claim 2
Dependent Claim
32 words
An improved Bacillus licheniformis alkaline protease, wherein at least one tryptophan has been genetically substituted by tyrosine or phenylalanine, whereby said protease is made stable against cleavage by hypochlorite at said residue.
Claim 3
Dependent Claim
15 words
The alkaline protease of claim 1, wherein the alkaline protease is isolated from Bacillus licheniformis.
Claim 4
Dependent Claim
21 words
The alkaline protease of claim 1, wherein the alkaline protease, prior to substitutions, is APL, as set forth in FIG. 3.
Claim 5
Dependent Claim
37 words
A bleach stable detergent composition comprising improved Bacillus licheniformis alkaline protease, wherein at least one tryptophan has been genetically substituted by tyrosine or phenylalanine, whereby said protease is made stable against cleavage by hypochlorite at said residue.
Claim 6
Dependent Claim
37 words
A bleach stable detergent composition comprising improved Bacillus licheniformis alkaline protease, wherein at least one tryptophan has been genetically substituted by tyrosine or phenylalanine, whereby said protease is made stable against cleavage by hypochlorite at said residue.
Claim 7
Dependent Claim
16 words
The bleach stable detergent of claim 4, wherein the alkaline protease is isolated from Bacillus licheniformis.
Claim 8
Dependent Claim
22 words
The bleach stable detergent of claim 4, wherein the alkaline protease, prior to substitution, is APL, as set forth in FIG. 3.
References Cited (Backward Citations)
US3623957A
Cited
EP0130756B1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-06-02 | 1995-12-02 | 1996-06-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-06-02 | 1999-12-02 | 2000-06-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-06-02 | 2003-12-02 | 2004-06-02 | $7700.00 | Window Closed / Lapsed |