US5116741A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosynthetic uses of thermostable proteases
Assignee: Genex Corp
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Filing: 1989-01-26
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Granted: 1992-05-26
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Expires: 2009-01-26
Abstract
Abstract The invention relates to the novel use of mutants of subtilisin in organic syntheses reactions in non-native environments. Especially the invention relates to methods for the use of mutant subtilisins in organic solvents for the catalysis of reactions involving ester formation and cleavage, including acylations and deacylations, and amidations and deamidations. The methods provide novel strategies which are useful in the synthesis of deoxynucleosides, dideoxynucleosides, peptides, sugars and the like.
Inventors
- 👤 Philip N. Bryan
- 👤 Michael W. Pantoliano
- 👤 Michele L. Rollence
- 👤 Chi H. Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 20:23
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/02; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
48 words
A method for the formation of a 5'-acylnucleoside, comprising condensing an enol ester with the sugar residue of a nucleoside in a reaction medium comprising an organic solvent, wherein the reaction is catalyzed by subtilisin mutein 8350 which is stable relative to native subtilisin in said reaction medium.
Claim 2
Independent Claim
48 words
A method for the formation of a 5'-acylnucleoside, comprising condensing an enol ester with the sugar residue of a nucleoside in a reaction medium comprising an organic solvent, wherein the reaction is catalyzed by subtilisin mutein 8350 which is stable relative to native subtilisin in said reaction medium.
Claim 3
Dependent Claim
24 words
The method of claim 1, wherein said nucleoside is deoxyadenosine, adenosine, deoxyinosine, inosine, deoxycytidine, cytidine, 2'-azido-2'-deoxycytidine, thymidine, 3'azido-3'-deoxythymidine, deoxyguanosine, guanosine, uridine, 2'-azido-2'-deoxyuridine or pseudouridine.
Claim 4
Dependent Claim
12 words
The method of claim 1, wherein said reaction medium is anhydrous DMF.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-05-26 | 1995-11-25 | 1996-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-05-26 | 1999-11-25 | 2000-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-05-26 | 2003-11-25 | 2004-05-26 | $7700.00 | Window Closed / Lapsed |