US5109124A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Nucleic acid probe linked to a label having a terminal cysteine
Assignee: Biogen Inc
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Filing: 1989-02-02
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Granted: 1992-04-28
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Expires: 2009-02-02
Abstract
Abstract A polynucleotide probe with a label bearing a plurality of signalling moieties. The label is attached to the probe by the reaction of an amino and sulfhydryl reactive hetero bifunctional reagent with the probe and label, the reaction resulting in the oxidation of a sulfhydryl group of the label. The label may be attached to the 5' terminus of the probe, or to modified bases of the probe. Probes constructed according to the invention are useful in detecting target sequences in genomic DNA.
Inventors
- 👤 Kuzhalmannam L. Ramachandran
- 👤 Richard L. Cate
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 04:14
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/088; A61K51/10; A61K51/1093; C; C07; C07H; C07H21/00; A61K2121/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
92 words
A labeled probe comprising a polynucleotide sequence complementary to a target polynucleotide sequence, a label, including aterminal cysteine residue, and a linkage group, the label and linkage group not interfering substantially with the characteristic ability of the probe polynucleotide sequence to hybridize to the target polynucleotide sequence, the label being covalently attached to the linkage group through a sulfur-carbon single bond, the sulfur atom of which is part of the terminal cysteine residue of the label, and the linkage group being covalently attached to the polynucleotide sequence through a carbon-oxygen single bond.
Claim 2
Dependent Claim
92 words
A labeled probe comprising a polynucleotide sequence complementary to a target polynucleotide sequence, a label, including aterminal cysteine residue, and a linkage group, the label and linkage group not interfering substantially with the characteristic ability of the probe polynucleotide sequence to hybridize to the target polynucleotide sequence, the label being covalently attached to the linkage group through a sulfur-carbon single bond, the sulfur atom of which is part of the terminal cysteine residue of the label, and the linkage group being covalently attached to the polynucleotide sequence through a carbon-oxygen single bond.
Claim 3
Dependent Claim
17 words
The labeled probe of claim 1, wherein the label comprises a polypeptide, including a terminal cysteine residue.
Claim 4
Dependent Claim
17 words
The labeled probe of claim 1, wherein at least one signalling moiety is attached to the label.
Claim 5
Dependent Claim
25 words
The labeled probe of claim 3, wherein at least one signalling moiety is attached to the label by way of at least one bridging moiety.
Claim 6
Dependent Claim
27 words
The labeled probe of claim 4, wherein the bridging moiety is selected from the group consisting of biotin, iminobiotin, avidin, streptavidin and an antibody to the label.
Claim 7
Dependent Claim
27 words
The labeled probe of claim 3, 4 or 5, wherein the signalling moiety is selected from the group consisting of radioactive isotopes, enzymes, coumarin, and fluorescent compounds.
Claim 8
Dependent Claim
23 words
The labeled probe of claim 1, 2, 3 or 4 wherein the linkage group is attached at the 5' terminus of the polynucleotide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-04-28 | 1995-10-28 | 1996-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-04-28 | 1999-10-28 | 2000-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-04-28 | 2003-10-28 | 2004-04-28 | $7700.00 | Window Closed / Lapsed |