EP0070686A2
Expired / Lapsed
EP
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Non-radiative energy transfer immunochemical technique
Assignee: BP Corp North America Inc
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Filing: 1982-07-14
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Granted: —
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Expires: 2002-07-14
Abstract
Abstract Antigen assays are performed by introducing an antigen- containing sample into a reagent solution comprising an absorber-emitter conjugated antibody and a chemiluminescent catalyst conjugated antibody, wherein the conjugated antibodies bind to the antigen in close proximity to enable transfer of energy to the absorber-emitter. The resulting emission of light from the absorber-emitter is related to the amount of antigen present in the sample.
Inventors
- 👤 Larry Edward Morrison
- 👤 Michael James Heller
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 10:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
58 words
A method for assaying antigens having multiple binding sites comprising: a) introducing a sample into a reagent solution comprising an absorber/emitter conjugated antibody, a chemiluminescent catalyst conjugated antibody, and chemiluminescent reagents suitable for inducing a light response in the presence of the chemiluminescent catalyst conjugated antibody; and b) detecting any light response emitted by the absorber/emitter conjugated antibody.
Claim 2
Independent Claim
58 words
A method for assaying antigens having multiple binding sites comprising: a) introducing a sample into a reagent solution comprising an absorber/emitter conjugated antibody, a chemiluminescent catalyst conjugated antibody, and chemiluminescent reagents suitable for inducing a light response in the presence of the chemiluminescent catalyst conjugated antibody; and b) detecting any light response emitted by the absorber/emitter conjugated antibody.
Claim 3
Dependent Claim
17 words
The method of Claim 1 wherein the chemiluminescent reagents comprise a chemiluminescent cofactor generator catalyst conjugated antibody.
Claim 4
Dependent Claim
15 words
The method of Claim 2 wherein the chemiluminescent reagents comprise a chemiluminescent cofactor scavenger catalyst.
Claim 5
Independent Claim
13 words
The method of any preceding claim wherein the absorber/ emitter is a fluorophore.
Claim 6
Dependent Claim
28 words
The method of Claim 4 wherein the fluorophore is selected from the group consisting of uroporphyrin, tetracarboxyphenylporphyrin, perylene, anthracene, 7-methyldibenzo (a,h) pyrene, 9,10-dibromoanthracene, riboflavin, 3,9-dibromoperylene, 3,10-dibromoperylene, and 3,10-dibromo-4,9-perylenedicarboxylate.
Claim 7
Independent Claim
16 words
The method of any of Claims 1 to 3 wherein the absorber emitter is a phosphore.
Claim 8
Dependent Claim
19 words
The method of Claim 6 wherein the phosphore is selected from the group consisting of 2,3-butadione, 1-[carboxyphenyl]-1,2-pentanedione, and 1-phenyl-1,2-propanedione.
Claim 9
Independent Claim
23 words
The method of any preceding claim wherein the chemiluminescent catalyst is selected from the group consisting of peroxidase, bacterial luciferase, and firefly luciferase.
Claim 10
Dependent Claim
36 words
A reagent solution for assaying large antigens comprising an absorber/emitter conjugated antibody, a chemiluminescent catalyst conjugated antibody, and chemiluminescent reagents necessary for inducing a chemiluminescent light response in the presence of the chemiluminescent catalyst conjugated antibody.
Claim 11
Dependent Claim
36 words
A reagent solution for assaying large antigens comprising an absorber/emitter conjugated antibody, a chemiluminescent catalyst conjugated antibody, and chemiluminescent reagents necessary for inducing a chemiluminescent light response in the presence of the chemiluminescent catalyst conjugated antibody.
Claim 12
Dependent Claim
18 words
The reagent solution of Claim 9 wherein the chemiluminescent reagents comprise a chemiluminescent cofactor generator catalyst conjugated antibody.
Claim 13
Dependent Claim
16 words
The reagent solution of Claim 10 wherein the chemiluminescent reagents comprise a chemiluminescent cofactor scavenger catalyst.
Claim 14
Dependent Claim
18 words
A reagent solution for assaying large antigens comprising peroxidase conjugated antibodies, uroporphyrin conjugated antibodies, luminol, and hydrogen peroxide.
Claim 15
Dependent Claim
18 words
A reagent solution for assaying large antigens comprising peroxidase conjugated antibodies, uroporphyrin conjugated antibodies, luminol, and hydrogen peroxide.
Claim 16
Dependent Claim
22 words
A reagent solution for assaying large antigens comprising peroxidase conjugated antibodies, riboflavin conjugated antibodies, luminol, glucose oxidase conjugated antibodies, glucose, and catalase.
Claim 17
Dependent Claim
22 words
A reagent solution for assaying large antigens comprising peroxidase conjugated antibodies, riboflavin conjugated antibodies, luminol, glucose oxidase conjugated antibodies, glucose, and catalase.
Claim 18
Dependent Claim
19 words
A reagent solution for assaying large antigens comprising peroxidase conjugated antibodies, uroporphyrin conjugated antibodies, isobutyraldehyde, dissolved oxygen, and ethanol.
Claim 19
Dependent Claim
19 words
A reagent solution for assaying large antigens comprising peroxidase conjugated antibodies, uroporphyrin conjugated antibodies, isobutyraldehyde, dissolved oxygen, and ethanol.
Claim 20
Dependent Claim
23 words
A reagent solution for assaying large antigens comprising firefly luciferase conjugated antibodies, uroporphyrin conjugated antibodies, adenosine triphosphate, luciferin, magnesium ions, and dissolved oxygen.
Claim 21
Dependent Claim
23 words
A reagent solution for assaying large antigens comprising firefly luciferase conjugated antibodies, uroporphyrin conjugated antibodies, adenosine triphosphate, luciferin, magnesium ions, and dissolved oxygen.
Claim 22
Dependent Claim
39 words
A reagent solution for assaying small antigens comprising effective amounts of an absorber/emitter conjugated antigen, a chemiluminescent catalyst conjugated antibody, and chemiluminescent reagents necessary for inducing a chemiluminescent light response in the presence of the chemiluminescent catalyst conjugated antibody.
Claim 23
Dependent Claim
39 words
A reagent solution for assaying small antigens comprising effective amounts of an absorber/emitter conjugated antigen, a chemiluminescent catalyst conjugated antibody, and chemiluminescent reagents necessary for inducing a chemiluminescent light response in the presence of the chemiluminescent catalyst conjugated antibody.
Claim 24
Dependent Claim
40 words
A reagent solution for assaying small antigens comprising effective amounts of a chemiluminescent catalyst conjugated antigen, an absorber/ emitter conjugated antibody, and chemiluminescent reagents necessary for inducing a chemiluminescent light response in the presence of the chemiluminescent catalyst conjugated antigen.
Claim 25
Dependent Claim
40 words
A reagent solution for assaying small antigens comprising effective amounts of a chemiluminescent catalyst conjugated antigen, an absorber/ emitter conjugated antibody, and chemiluminescent reagents necessary for inducing a chemiluminescent light response in the presence of the chemiluminescent catalyst conjugated antigen.
References Cited (Backward Citations)
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